响应面法优化酶-水连续提取西洋参须根人参皂苷

doi:10.3976/j.issn.1002-4026.20240113

Abstract

Abstract:

To optimize the extraction process for ginsenosides from the fibrous roots of American ginseng，the enzyme-water method was used for continuous extraction of ginsenosides from American ginseng. The total ginsenosides extraction yield was used as the evaluation index，and the extraction process was optimized using the response surface methodology. The results showed that the optimal enzymatic extraction conditions were as follows： α-amylase with an enzyme concentration of 7.3%，a liquid/solid ratio of 30 mL/g，and an enzymatic hydrolysis time of 1.2 h. After enzymatic hydrolysis，a secondary water extraction was performed，and the optimal conditions were as follows： a liquid/solid ratio of 27 mL/g，an extraction temperature of 89 ℃，and an extraction time of 1.3 h. Under these conditions，the total ginsenosides extraction yield reached 16.61%. Since it is stable，simple，and efficient，this method has significantly improved the extraction yield and avoided the waste of resources. It partially fills a gap in the extraction process of total ginsenosides from the fibrous roots of American ginseng and provides a reference for further purification，refinement，and activity studies of total ginsenosides.

Key words: American ginseng, fibrous roots, ginsenosides, extraction process, response surface methodology, single factor experiment, ultraviolet spectrophotometry

CLC Number:

R284.2

WANG Haiyan, YU Ping, WANG Xin, YU Hongxia, ZHOU Jie, FANG Lei. Optimization of enzyme-water continuous extraction of ginsenosides from fibrous roots of American ginseng using response surface methodology[J].Shandong Science, 2025, 38(5): 10-21.

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References 26

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条件	α-淀粉酶	木瓜蛋白酶
液料比/（mL·g^-1）	7	5
酶解温度/℃	85	55
酶解时间/h	2	4
加酶量/%	2.0	0.4

水平	因素
水平	A加酶量/%	B液料比/（mL·g^-1）	C酶解时间/h
-1	5	20	0.5
0	7	25	1.0
1	9	30	1.5

水平	因素
水平	A液料比	B提取温度/℃	C提取时间/h
-1	20	70	0.5
0	25	80	1.0
1	30	90	1.5

实验号	A液料比	B提取温度	C提取时间	Y总皂苷提取率/%
1	-1	-1	0	12.01
2	1	-1	0	12.45
3	-1	1	0	14.16
4	1	1	0	14.17
5	-1	0	-1	13.16
6	1	0	-1	12.51
7	-1	0	1	12.67
8	1	0	1	13.89
9	0	-1	-1	12.51
10	0	1	-1	13.61
11	0	-1	1	12.81
12	0	1	1	14.43
13	0	0	0	14.56
14	0	0	0	14.41
15	0	0	0	14.21
16	0	0	0	14.49
17	0	0	0	14.32

来源	离均差平方和	自由度	均方	F	P	显著性
模型	12.290	9	1.370	38.24	<0.000 1	**
A	0.130	1	0.130	3.64	0.098 0
B	5.430	1	5.430	152.00	<0.000 1	**
C	0.510	1	0.510	14.14	0.007 1	**
AB	0.046	1	0.046	1.29	0.292 7
AC	0.870	1	0.870	24.48	0.001 7	**
BC	0.068	1	0.068	1.89	0.211 3
A²	2.320	1	2.320	64.82	<0.000 1	**
B²	0.890	1	0.890	24.84	0.001 6	**
C²	1.510	1	1.510	42.30	0.000 3	**
残差	0.250	7	0.036
失拟项	0.170	3	0.058	3.04	0.155 6
纯误差	0.076	4	0.019
总和	12.540	16

Optimization of enzyme-water continuous extraction of ginsenosides from fibrous roots of American ginseng using response surface methodology

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实验号	A液料比	B提取温度	C提取时间	Y总皂苷提取率/%
1	-1	-1	0	14.91
2	1	-1	0	15.43
3	-1	1	0	15.48
4	1	1	0	15.75
5	-1	0	-1	14.82
6	1	0	-1	15.53
7	-1	0	1	15.47
8	1	0	1	15.79
9	0	-1	-1	14.94
10	0	1	-1	15.58
11	0	-1	1	15.48
12	0	1	1	15.81
13	0	0	0	15.68
14	0	0	0	15.57
15	0	0	0	15.63
16	0	0	0	15.59
17	0	0	0	15.63

来源	离均差平方和	自由度	均方	F	P	显著性
模型	1.430	9	0.160	63.12	<0.0001	**
A	0.410	1	0.410	164.21	<0.000 1	**
B	0.430	1	0.430	175.51	<0.000 1	**
C	0.350	1	0.350	139.92	<0.000 1	**
AB	0.016	1	0.016	6.20	0.041 6	*
AC	0.038	1	0.038	15.08	0.006 0	**
BC	0.024	1	0.024	9.53	0.017 6	*
A²	0.081	1	0.081	32.15	0.000 8	**
B²	0.033	1	0.033	13.15	0.008 4	**
C²	0.026	1	0.026	10.36	0.014 7	*
残差	0.018	7	2.521×10^-3
失拟项	0.010	3	3.483×10^-3	1.94	0.265 7
纯误差	7.20×10^-3	4	1.800×10^-3
总和	1.450	16

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