当归挥发油的提取工艺优化及抗氧化性研究

doi:10.3976/j.issn.1002-4026.2022.06.003

Abstract

Abstract:

To determine the most optimal extraction process for angelica volatile oil and its antioxidant activity. In this experiment, authentic Angelica sinensis was used as the test material and the single factor and response surface analysis methods were adopted. The particle size of angelica granules, water addition ratio, and extraction time were investigated. In addition, volatile oil yield was evaluated, and the volatile oil extraction process was optimized. Under the conditions of this process, the volatile oil of A. sinensis was extracted, and the antioxidant activity of the volatile oil was determined based on the DPPH free radical scavenging rate. The results showed that the optimum process parameters were particle size of 24 mesh, water addition ratio of 6, and extraction time of 7 h. Under these conditions, the extraction rate of volatile oil from A. sinensis was high.The antioxidant assay showed that when the concentration of A. sinensis volatile oil was 1.2 mg/mL, the DPPH radical scavenging rate was 51.9%. The process optimized by the single factor and response surface model was stable, and the extraction rate of the volatile oil was high. The volatile oil produced under these conditions had good antioxidant activity, which can provide scientific and rational guidance for clarifying and optimizing the process parameters for extracting A. sinensis volatile oil.

Key words: Angelica sinesis, volatile oil, response surface methodology, antioxidant

CLC Number:

R284.2

GUO Tian-tian, FANG Zhao-ying, LIU Zhen-yuan, ZHANG Wei, BI Yan-yan, SUN Yan, GUAN Yong-xia. Optimization of extraction process and antioxidant activity of Angelica sinensis volatile oil[J].Shandong Science, 2022, 35(6): 16-23.

Figures/Tables 7

Table 1

Fig.1

Table 2

Table 3

Significance test and variance analysis of regression model"

方差来源	平方和	自由度	均方	F值	p值	显著性
模型	9.14×10^-3	9	1.02×10^-3	71.08	< 0.000 1	**
A当归粒径	3.51×10^-4	1	3.51×10^-4	24.57	0.001 6	**
B加水倍数	4.96×10^-4	1	4.96×10^-4	34.71	0.000 6	**
C提取时间	1.74×10^-3	1	1.74×10^-3	121.77	< 0.000 1	**
AB	9.00×10^-6	1	9.00×10^-6	0.63	0.453 5
AC	1.33×10^-4	1	1.33×10^-4	1.72	0.196 3
BC	2.03×10^-5	1	2.03×10^-5	1.42	0.272 7
A²	3.44×10^-3	1	3.44×10^-3	240.96	< 0.000 1	**
B²	1.70×10^-3	1	1.70×10^-3	119.02	< 0.000 1	**
C²	7.50×10^-4	1	7.50×10^-4	52.50	0.000 2	**
残差	1.00×10^-4	7	1.43×10^-5
失拟项	8.13×10^-5	3	2.71×10^-5	5.76	0.061 9
误差项	1.88×10^-5	4	4.70×10^-6
总和	9.24×10^-3	16
R²=0.989 2, $R A d j 2$ =0.975 3

Table 3

Fig.2

Table 4

Fig.3

References 21

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试验号	A:当归粒径/目	B:加水倍数	C:提取时间/h	挥发油提取率/%
1	0	0	0	0.546
2	0	-1	1	0.517
3	1	0	-1	0.482
4	1	0	1	0.511
5	-1	0	-1	0.494
6	0	0	0	0.545
7	0	0	0	0.544
8	1	-1	0	0.481
9	0	1	-1	0.504
10	0	1	1	0.533
11	0	0	0	0.546
12	1	1	0	0.497
13	-1	1	0	0.509
14	-1	0	1	0.523
15	0	0	0	0.545
16	0	-1	-1	0.489
17	-1	-1	0	0.494

当归批号	挥发油提取率/%
210703	0.538
210717	0.537
210728	0.538

Optimization of extraction process and antioxidant activity of Angelica sinensis volatile oil

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因素	水平
因素	-1	0	1
A:当归粒径/目	10	24	50
B:加水倍数/倍	5	6	7
C:提取时间/h	6	7	8

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