Box-Behnken Design响应面法优化银杏叶总黄酮柱层析纯化工艺

doi:10.3976/j.issn.1002-4026.2022.06.002

Abstract

Abstract:

The study’s aim was to obtain Ginkgo biloba extract with a high mass fraction of total flavonoids. On the basis of single-factor test, the sample concentration, ethanol volume fraction, eluate volume, and elution flow rate were used as the investigation factors and the mass fraction and yield of total flavonoids extracted from G. biloba were used as the investigation indicators. Box-Behnken design (BBD) and response surface method were used to optimize the column chromatography process for purifying the total flavonoids extracted from G. biloba. Optimal purification was achieved by using a sample concentration of 3.1 mg/mL, ethanol volume fraction of 71%, eluate volume of 2 BV (BV is the column void volume), and elution flow rate of 2 BV/h. Under the optimal conditions, three batches of verification experiments were conducted. The average mass fraction of total flavonoids in the G. biloba extract reached 35.77%, and the yield was 93.30%. The mass fraction of total lactones in the G. biloba extract reached the standards of The Pharmacopoeia of the People's Republic of China. Using BBD and response surface method for optimizing the column chromatography process for the purification of total flavonoids is scientific, reasonable, and feasible.This study can be used as a reference for the industrial production of total flavonoids.

Key words: Box-Behnken Design and response surface method, macroporous resin, column chromatography, total flavonoids from Ginkgo biloba, purification

CLC Number:

R284.2

BI Yan-yan, MA Qing-wen, XU Hui, LIU Jian, LONG Rui, TANG Wei-ming, GUO Tian-tian, HAO Da-wei. Optimization of the column chromatographic purification of total flavonoids extracted from Ginkgo biloba using Box-Behnken design and response surface method[J].Shandong Science, 2022, 35(6): 8-15.

Figures/Tables 5

Fig.1

Fig.2

Fig.3

Table 1

Fig.4

差异来源	自由度	R_综
差异来源	自由度	平方和	均方	F值	P值
模型	14	1 683.99	120.29	134.47	<0.000 1
A	1	65.10	65.10	72.78	<0.000 1
B	1	13.89	13.89	15.53	0.001 5
C	1	1.72	1.72	1.92	0.187 5
D	1	2.92	2.92	3.26	0.092 3
AB	1	4.80	4.80	5.36	0.036 3
AC	1	0.10	0.10	0.11	0.740 1
AD	1	1.27	1.27	1.41	0.254 0
BC	1	7.21	7.21	8.06	0.013 1
BD	1	0.49	0.49	0.55	0.471 5
CD	1	7.70	7.70	8.61	0.010 9
A²	1	465.73	465.73	520.66	<0.000 1
B²	1	1 074.99	1 074.99	1201.78	<0.000 1
C²	1	531.23	531.23	593.89	<0.000 1
D²	1	273.61	273.61	305.88	<0.000 1
残差	14	12.52	0.89
失拟项	10	10.44	1.04	2.01	0.261 9
纯误差	4	2.08	0.52
总差	28	1 696.52

[1]	国家药典委员会. 中华人民共和国药典2020年版一部[M]. 北京: 中国医药科技出版社, 2020.
[2]	钦富华, 黄孝闻, 夏晓静, 等. 大孔吸附树脂法同时纯化银杏叶中黄酮苷和萜类内酯[J]. 广东药学院学报, 2013, 29(2): 142-146. DOI:10.3969/j.issn.1006-8783.2013.02.008. doi: 10.3969/j.issn.1006-8783.2013.02.008
[3]	张鹏飞, 廖丽君, 邓祯, 等. 银杏叶提取物的药理作用及其临床应用研究进展[J]. 辽宁中医杂志, 2017, 44(2):426-429. DOI:10.13192/j.issn.1000-1719.2017.02.069. doi: 10.13192/j.issn.1000-1719.2017.02.069
[4]	胡春苗, 刘晋仙, 郭瑞齐, 等. 银杏叶总黄酮提取纯化技术的研究进展[J]. 时珍国医国药, 2015, 26(9): 2225-2227.
[5]	倪力军, 颜玮韬, 张立国. 银杏叶黄酮含量对银杏叶提取物大孔树脂纯化工艺条件的影响研究[J]. 天然产物研究与开发, 2017, 29(12): 2097-2103. DOI:10.16333/j.1001-6880.2017.12.017. doi: 10.16333/j.1001-6880.2017.12.017
[6]	盖晓红, 刘素香, 任涛, 等. 银杏化学成分、制剂种类和不良反应的研究进展[J]. 药物评价研究, 2017, 40(6): 742-751. DOI:10.7501/j.issn.1674-6376.2017.06.003. doi: 10.7501/j.issn.1674-6376.2017.06.003
[7]	牟玲丽, 寇俊萍, 朱丹妮, 等. 银杏叶的化学成分及其抗氧化活性[J]. 中国天然药物, 2008, 6(1): 26-29.
[8]	蔡锦源, 王萌璇, 韦坤华, 等. 银杏黄酮的提取及纯化方法研究进展[J]. 应用化工, 2017, 46(5): 982-985. DOI:10.3969/j.issn.1671-3206.2017.05.041. doi: 10.3969/j.issn.1671-3206.2017.05.041
[9]	陈三达, 乔娟娟, 陆耕宇, 等. 大孔树脂富集纯化黄酮类化合物的研究进展[J]. 广州化工, 2021, 49(17): 9-13. DOI:10.3969/j.issn.1001-9677.2021.17.005. doi: 10.3969/j.issn.1001-9677.2021.17.005
[10]	王晓娅, 苏建青, 褚秀玲. 大孔树脂分离纯化黄酮类化合物的研究进展[J]. 食品科技, 2021, 46(8): 208-212. DOI:10.13684/j.cnki.spkj.2021.08.031. doi: 10.13684/j.cnki.spkj.2021.08.031
[11]	冯靖, 彭效明, 李翠清, 等. 大孔树脂纯化银杏叶中总黄酮的工艺研究[J]. 应用化工, 2019, 48(10): 2339-2343. DOI:10.16581/j.cnki.issn1671-3206.2019.10.006. doi: 10.16581/j.cnki.issn1671-3206.2019.10.006
[12]	冯玉康, 王文鹏, 郝鹏彬, 等. 响应曲面法优化银杏叶提取物柱层析精制工艺[J]. 中国现代中药, 2015, 17(12): 1321-1326. DOI:10.13313/j.issn.1673-4890.2015.12.023. doi: 10.13313/j.issn.1673-4890.2015.12.023
[13]	耿嘉, 刘越敏, 孙雅馨, 等. 响应曲面分析法研究5-FU温热化疗对BGC-823细胞增殖的影响[J]. 中国现代应用药学, 2020, 37(12): 1433-1437. DOI:10.13748/j.cnki.issn1007-7693.2020.12.005. doi: 10.13748/j.cnki.issn1007-7693.2020.12.005
[14]	高媛, 邢文馨, 王慧梅. 大孔树脂纯化岩高兰多酚的工艺研究[J]. 植物研究, 2021, 41(4): 626-632. DOI:10.7525/j.issn.1673-5102.2021.04.018. doi: 10.7525/j.issn.1673-5102.2021.04.018
[15]	李慧敏, 牛东攀, 陈丹阳, 等. 响应面法优化大孔树脂纯化杜仲绿原酸工艺研究[J]. 食品研究与开发, 2018, 39(13): 40-46. DOI:10.3969/j.issn.1005-6521.2018.13.008. doi: 10.3969/j.issn.1005-6521.2018.13.008

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Optimization of the column chromatographic purification of total flavonoids extracted from Ginkgo biloba using Box-Behnken design and response surface method

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