基于DPPHHPLC技术的马粪海胆抗氧化活性指纹图谱初步研究

doi:10.3976/j.issn.1002-4026.2014.03.002

J4 ›› 2014, Vol. 27 ›› Issue (3): 7-11.doi: 10.3976/j.issn.1002-4026.2014.03.002

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

DPPH-HPLC based preliminary research on antioxidantactivity fingerprint for Hemicentrotus pulcherrimus(A. Agassiz)

ZHAO Heng-Qiang¹, CHEN Jun-Hui², GENG Yan-Ling¹, LIU Jian-Hua¹, WANG Xiao^1*

1. Shandong Provincial Key Laboratory of Applied Technology of Sophisticated Analytical Instruments,Process Control Research Center for Traditional Chinese Medicine, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014,China; 2. Qingdao Key Labortory of Analytical Technology Development and Standardization of Chinese Medicines,
The First Institute of Oceanography, SOA, Qingdao 266061, China

Received:2014-01-15 Online:2014-06-20 Published:2014-06-20

Abstract

Abstract:

We identify the origin of Hemicentrotus pulcherrimus(A. Agassiz) with a DPPH-HPLC based antioxidant fingerprint. DPPH-HPLC was employed to screen the antioxidant of water extract of Hemicentrotus pulcherrimus.Antioxidant based fingerprint combined with similarity analysis was employed to identify the authenticity of different Hemicentrotus pulcherrimus. Results show that the approach can distinguish Hemicentrotus pulcherrimus from different origins. Thus, it has the potential to become a new method for the identification of marine Chinese medicines.

Key words: Hemicentrotus pulcherrimus(A. Agassiz), DPPH-HPLC, antioxidant, fingerprint

CLC Number:

R284.1

ZHAO Heng-Qiang, CHEN Jun-Hui, GENG Yan-Ling, LIU Jian-Hua, WANG Xiao. DPPH-HPLC based preliminary research on antioxidantactivity fingerprint for Hemicentrotus pulcherrimus(A. Agassiz)[J].J4, 2014, 27(3): 7-11.

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DPPH-HPLC based preliminary research on antioxidantactivity fingerprint for Hemicentrotus pulcherrimus(A. Agassiz)

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