SHANDONG SCIENCE ›› 2018, Vol. 31 ›› Issue (4): 20-25.doi: 10.3976/j.issn.1002-4026.2018.04.004

• Traditional Chinese medicine and natural active products • Previous Articles     Next Articles

Determination of chemical components in flower buds and leaves of Lonicera japonica in Tibet area by HPLC-DAD

LIU Qian1,ZHANG Min-min1,LI Sheng-bo2,LIANG Yan1,3,LIU Wei1,CHENG Su-pan1,4,WANG Xiao1,GENG Yan-ling1,ZHAO Heng-qiang1*
  

  1. 1. Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; 2. Qinghai Yate Ecotech Co. LTD., Xining 276017, China; 3. College of Food Science and Engineering, Shandong Agricultural University, Taian 2710183,China;4. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2017-12-28 Online:2018-08-20 Published:2018-08-20

Abstract:

The active components of flower buds and leaves of Lonicera japonica “AT1”in Tibet area were analyzed to provide data support for their quality evaluation and exploitation. High performance liquid chromatography diode array detector (HPLC-DAD) was used to analyze 8 batches of Lonicera japonica Thunb. Flower buds and leaves in Tibet. The 8 main compounds of chlorogenic acid, luteoloside, loganin acid, cryptochlorogenic acid, loganin determination, Secoxyloganin,
rutin, and isochlorogenic acid C content were measured, respectively. The buds were 3.85%, 0.15%,0.18%, 0.15%, 0.11%, 0.11%, 0.17% and 0.11%, and leaves: 3.58%, 0.79%,0.40%, 0.08%, 0.07%, 0.24%, 0.18% and 0.05%. This study provides a method and data support for the rapid detection of
chemical constituents and further exploitation of the fine species of Lonicera japonica in Tibet.

Key words: HPLC-DAD, Lonicera japonica “AT1”, determination of content, fingerprint

CLC Number: 

  • R284.1