HPLC-DAD法测定西藏产区金银花花蕾及叶中化学成分含量

doi:10.3976/j.issn.1002-4026.2018.04.004

Abstract

Abstract:

The active components of flower buds and leaves of Lonicera japonica “AT1”in Tibet area were analyzed to provide data support for their quality evaluation and exploitation. High performance liquid chromatography diode array detector (HPLC-DAD) was used to analyze 8 batches of Lonicera japonica Thunb. Flower buds and leaves in Tibet. The 8 main compounds of chlorogenic acid, luteoloside, loganin acid, cryptochlorogenic acid, loganin determination, Secoxyloganin,

rutin, and isochlorogenic acid C content were measured, respectively. The buds were 3.85%, 0.15%,0.18%, 0.15%, 0.11%, 0.11%, 0.17% and 0.11%, and leaves: 3.58%, 0.79%,0.40%, 0.08%, 0.07%, 0.24%, 0.18% and 0.05%. This study provides a method and data support for the rapid detection of

chemical constituents and further exploitation of the fine species of Lonicera japonica in Tibet.

Key words: HPLC-DAD, Lonicera japonica “AT1”, determination of content, fingerprint

CLC Number:

R284.1

LIU Qian，ZHANG Min-min，LI Sheng-bo，LIANG Yan，LIU Wei，CHENG Su-pan，WANG Xiao，GENG Yan-ling，ZHAO Heng-qiang. Determination of chemical components in flower buds and leaves of Lonicera japonica in Tibet area by HPLC-DAD[J].SHANDONG SCIENCE, 2018, 31(4): 20-25.

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Determination of chemical components in flower buds and leaves of Lonicera japonica in Tibet area by HPLC-DAD

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