桂枝HPLC指纹图谱研究

doi:10.3976/j.issn.1002-4026.2013.05.010

J4 ›› 2013, Vol. 26 ›› Issue (5): 47-51.doi: 10.3976/j.issn.1002-4026.2013.05.010

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

HPLC fingerprint of Ramulus Cinnamomi

ZHAO Heng-Qiang¹, GENG Yan-Ling¹, WU Hong-Wei,² DUAN Wen-Juan¹, YANG Hong-Jun², LIU Jian-Hua¹, WANG Xiao^1*

1.Process Control Research Center of Traditional Chinese Medicine,Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 2. Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received:2013-04-24 Online:2013-10-20 Published:2013-10-20

Abstract

Abstract:

We constructed high performance liquid chromatography (HPLC) fingerprint for Ramulus Cinnamomi to provide a new method for its quality evaluation and identification. We analyzed thirteen batches of Ramulus Cinnamomi with C18 column (Agilent Eclipse XDBC18 column), gradient elution solvent system including water and acetonitrile, flow rate of 0.8 mL/min, column temperature of 25℃ and detection wavelength of 285 nm. The method had better precision, repetition and stability. Thirteen shared peaks and four specific peaks exist in the thirteen batches of fingerprints. Similarity analysis shows that the quality of Guangdong and Guangxi samples has slight difference. HPLC fingerprinting will be a potential identification and quality evaluation tool for Ramulus Cinnamomi.

Key words: Ramulus Cinnamomi, HPLC, fingerprint

CLC Number:

R284.1

ZHAO Heng-Qiang, GENG Yan-Ling, WU Hong-Wei, DUAN Wen-Juan, YANG Hong-Jun, LIU Jian-Hua, WANG Xiao. HPLC fingerprint of Ramulus Cinnamomi[J].J4, 2013, 26(5): 47-51.

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HPLC fingerprint of Ramulus Cinnamomi

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