基于UHPLC-Q-Exactive Orbitrap-MS技术的垂盆草化学成分表征与鉴定

doi:10.3976/j.issn.1002-4026.20230173

Abstract

Abstract:

To develop an ultra-performance liquid chromatograpy-quadrupole electrostatic field orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap-MS)method for characterization and identification of chemical constituents in Sedum sarmentosum Bung.The separation was performed using a Phenomenex Kinetex C₁₈ column(2.1 mm×100 mm, 2.6 μm) with a mobile phase comprising acetonitrile and water containing 0.1% formic acid via gradient elution at a flow rate of 0.3 mL/min;the injection volume was 3.0 μL.Mass spectrometry was performed in positive and negative ionization modes with electrospray ionization. The chemical constituents were characterized and identified based on retention time, precise molecular weight, fragment ions,and comparison with the reference substances.The results of this study showed that 53 compounds were characterized and identified in Sedum sarmentosum Bung, including 25 flavonoids, 12 megastigmanes,11 organic acids,3 alkaloids and 2 other compounds.Five of these compounds were identified via comparison with reference compounds, and three compounds may have not been reported from Sedum sarmentosum Bung.The UHPLC-Q-Exactive Orbitrap-MS method proposed in this study can rapidly and comprehensively characterize the chemical constituents in Sedum sarmentosum Bung and provide a reference basis for the research on quality control and atherapeutic material basis of Sedum sarmentosum Bung.

Key words: Sedum sarmentosum Bung, UHPLC-Q-Exactive Orbitrap-MS, chemical constituents, flavonoids

CLC Number:

R284

ZHU Xinyi, YANG Chunguo, TIAN Haitao, HOU Miao, HAN Liwen, DENG Zhipeng. Characterization and identification of chemical constituents in Sedum sarmentosum Bung based on UHPLC-Q-Exactive Orbitrap-MS technology[J].Shandong Science, 2024, 37(5): 10-16.

Figures/Tables 6

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References 29

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Characterization and identification of chemical constituents in Sedum sarmentosum Bung based on UHPLC-Q-Exactive Orbitrap-MS technology

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