吲哚乙酰化高直链玉米淀粉改善溃疡性结肠炎的作用机制研究

doi:10.3976/j.issn.1002-4026.20230176

Abstract

Abstract:

The aim of this study is to investigate the mechanism action of indole acetylated high-amylose maize starch in improving ulcerative colitis. Dextran sulfate sodium salt was used to induce acute ulcerative colitis in mice. Indole acetylated high-amylose maize starch was then used to target the colon with the delivery of indole-3-acetic acid. This was combined with the administration of an aryl hydrocarbon receptor inhibitor to analyze the mice in terms of colon length; disease activity index; and levels of interleukin-6, interleukin-10, transforming growth factor β1, myeloperoxidase, and interleukin-22 in the colon, which were detected by enzyme-linked immunosorbent assay. Quantitative polymerase chain reaction was used to analyze the relative expression levels of the colonic tight junction proteins occludin, ZO-1, and CYP1A1. Flow cytometry was used to test the ratio of regulatory T cells and T helper 17 cells in the mesenteric lymph nodes. The results showed that administration of indole acetylated high-amylose maize starch significantly alleviated the shortening of the colon length; significantly reduced the disease activity index and the levels of interleukin-6 and myeloperoxidase; significantly promoted the expression of interleukin-10, transforming growth factor β1, interleukin-22, CYP1A1, occludin, and ZO-1; significantly increased the proportion of regulatory T cells; and significantly reduced the proportion of T helper 17 cells. Administration of the aryl hydrocarbon receptor inhibitor weakened the effect of indole acetylated high-amylose maize starch. These results together suggest that acetylated high-amylose maize starch can improve ulcerative colitis by activating aryl hydrocarbon receptors.

Key words: microbiota metabolites, indole-3-acetic acid, ulcerative colitis, aryl hydrocarbon receptors, inflammatory response, immune balance, tryptophan

CLC Number:

R967

QU Xinyan, LI Qingjun, DING Xingchun, SONG Yingying. Mechanism of action indole acetylated high-amylose maize starch in improving ulcerative colitis[J].Shandong Science, 2024, 37(5): 17-24.

Figures/Tables 8

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References 21

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得分	体重下降/%	大便性状	粪便出血
0	≤1	正常	阴性
1	>1~5	松散	隐血阳性
2	>5~10	松散	隐血阳性
3	>10~20	稀便	肉眼血便
4	>20	稀便	肉眼血便

CD4⁺T细胞亚群	细胞表型特征
Naïve CD4⁺ T	CD3⁺ B220^- CD4⁺ CD8^- CD25^- CD44^low CD62L^high
Treg	CD3⁺ B220^- CD4⁺ CD8^- CD25⁺ FoxP3⁺
Th17	CD3⁺ B220^- CD4⁺ CD8^- CD25^- CD44⁺ IL-17a⁺

Mechanism of action indole acetylated high-amylose maize starch in improving ulcerative colitis

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