Abstract:

With the method of ultrasonicassisted extraction, the content and its change rule of total flavonoids in Trichosanthes kirilowii seeds during six stages of prophase imbibition, imbibition, germination, radicle elongation, bud breaking and seedling were studied. After degreased by petroleum ether, total flavonoids of Trichosanthes kirilowii seeds at different germination stage were extracted with 70% ethanol as solvent by ultrasonicassisted extraction, and determined using rutin as the standard by colorimetry method. The results showed that total flavonoids content in the seeds of Trichosanthes kirilowii was low, about 1 mg/g, and it showed tendency to descend firstly and then ascend during the whole germination process, rising to 2.7 mg/g at seeding stage. This study provides references for further understanding and utilization of Trichosanthes kirilowii seeds, and provides theoretical basis for artificial regulation of total flavonoids content in Trichosanthes kirilowii seeds.

Key words: Trichosanthes kirilowii seeds, germination process, total flavonoids