高速逆流色谱分离纯化灯盏细辛中的黄酮类化合物

doi:10.3976/j.issn.1002-4026.2015.03.003

SHANDONG SCIENCE ›› 2015, Vol. 28 ›› Issue (3): 11-15.doi: 10.3976/j.issn.1002-4026.2015.03.003

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Separation and purification of flavonoids from Erigeron breviscapus by highspeed countercurrent chromatography

SUN Changlei1, 2,WANG Zhaoping1,SUN Zhaolin1,YANG Peng3,WANG Xiao2,LI Jia1*, MOU Yuzhu4

1. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 3. Sanfeng Biological Engineering Technology Co. Ltd., Jinan 250014, China; 4.Yantai Zhangyu Pioneer Wine Co. Ltd., Yantai 264001, China

Received:2015-04-13 Online:2015-06-20 Published:2015-06-20

Abstract

Abstract: We rapidly separated and purified flavonoids from Erigeron breviscapus by highspeed counter current chromatography (HSCCC). The extraction conditions were solvent system of petroleum etherethyl acetatemethanolwater (4:6:3.5:6.5,V/V), rotation speed of 850 r/min,flow speed of 2 mL/min, and detection wavelength of 254 nm. Four flavonoids,baicalein (87 mg), luteolin (35 mg),quercetin (46 mg) and apigenin (92 mg) were separated from crude extract of 500 mg, whose purities are 94.6%, 92.5%, 98.2% and 97.5% by HPLC analysis. Experimental results show that the approach is rapid, highly efficient and purified.

Key words: highspeed countercurrent chromatography, flavonoids, Erigeron breviscapus

CLC Number:

R284.2

SUN Changlei1, 2,WANG Zhaoping1,SUN Zhaolin1,YANG Peng3,WANG Xiao2,LI Jia1*, MOU Yuzhu4. Separation and purification of flavonoids from Erigeron breviscapus by highspeed countercurrent chromatography[J].SHANDONG SCIENCE, 2015, 28(3): 11-15.

References

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Separation and purification of flavonoids from Erigeron breviscapus by highspeed countercurrent chromatography

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