基于液相色谱-质谱的西洋参不同部位人参皂苷差异性分析

doi:10.3976/j.issn.1002-4026.2025064

Abstract

Abstract: Ginsenosides are important chemical components ofPanax quinquefoliusL. and are closely related to its pharmacological activities. In this study, ultra-high performance liquid chromatography–mass spectrometry (UHPLC–MS) was used for high-throughput analysis of ginsenosides in the roots, leaves, and seeds ofPanax quinquefoliusL., leading to the identification of 72 ginsenosides across these parts. Both univariate and multivariate analyses revealed significant differences in the content and types of ginsenosides among the different plant parts. Based on statistical significance (p< 0.05) and biological significance (fold change > 4), 23 differential saponins were identified in the roots vs. leaves comparison, 26 in the roots vs. seeds comparison, and 27 in the leaves vs. seeds comparison. Content analysis revealed that the total amounts of Rg1, Re, and Rb1in the leaves and roots were roughly equivalent and higher than in the seeds, while most other ginsenosides were more abundant in the leaves and roots than in the seeds. The pseudo-ginsenoside F11, which is unique toPanax quinquefoliusL., was most abundant in the leaves, whereas acetylated pseudo-ginsenoside F11 was most abundant in the roots. 20(S)-Ginsenoside Rh1, acetylated ginsenoside Rg1, and quinquenoside IV were all significantly different among the three parts and could serve as markers for differentiation. This study reveals distinct ginsenoside profiles in the roots, leaves, and seeds ofPanax quinquefoliusL.,, providing technical support for its efficient development and utilization.

Key words: Panax quinquefolius L., ginsenosides, plant parts, ultra-high performance liquid chromatography–mass spectrometry (UHPLC–MS)

CLC Number:

R284.1

LIU Yuemeng, DONG Hongjing, XIE Yao, WANG Xiao, LIU Jing, LI Lili. Difference analysis of ginsenosides in different parts of Panax quinquefolius L. based on liquid chromatography –mass spectrometry[J].Shandong Science, 0, (): 1-.

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Difference analysis of ginsenosides in different parts of Panax quinquefolius L. based on liquid chromatography –mass spectrometry

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