Shandong Science ›› 2025, Vol. 38 ›› Issue (5): 22-30.doi: 10.3976/j.issn.1002-4026.20240104

• Traditional Chinese Medicine and Natural Active Products • Previous Articles     Next Articles

Quality control study of Jingfang granules through combined fingerprinting and comprehensive assessment of activity

MIAO Ran1a,1b(), GUAN Yongxia2, GUO Huanying1a,1b, ZHANG Guimin2, WANG Xiao1a,1b, ZHAO Hengqiang1a,1b,*()   

  1. 1. a. Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center;b. Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology (Shandong Academy of Sciences),Jinan 250014,China
    2. Lunan Pharmaceutical Group Co.,Ltd.,State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Linyi 276006,China
  • Received:2024-08-27 Revised:2024-10-12 Published:2025-10-20 Online:2025-09-22

Abstract:

Combined fingerprinting and comprehensive assessment of activity techniques were used in this study to determine the quality control of Jingfang granules. Separation was performed using an Agilent TC-C18 chromatographic column (4.6 mm×250 mm,5 μm),gradient elution performed using an acetonitrile-0.8% formic acid aqueous solution,and ESI-TOF/mass spectrometer was used for analysis. The chemical constituents of Jingfang granules were identified through the precise molecular weight information of the parent and daughter ions of compounds and by consulting references. Molecular docking was used to examine the bonding ability of the following five chemical componentsto neuraminidase (NA): prim-O-glucosylcimifugin (POG),ferulic acid,hesperidin,naringin,and sec-O-glucosylhamaudol (SOGH). The antioxidant activity of the five components was evaluated by an ABTS free radical scavenging in vitro experiment. Fingerprinting and quantitative assay were used to evaluate the quality of Jingfang granules. From the results,twenty-four chemical components were identified within the Jingfang granules. Molecular docking results revealed the high binding capacity of NA to POG,ferulic acid,naringin,and SOGH,with a minimum recorded binding energy of <-5 kcal/mol. Free radical scavenging in vitro experiments revealed that all the five components exhibited certain antioxidant activities. The compositional similarity of different batches of Jingfang granules was found to be relatively close,but there were some differences in the content of the five components,which may be attributed to differences in the sources of Chinese herbal medicines.This study provides methodological and conceptual support for overall quality evaluation studies of Jingfang granules.

Key words: Jingfang granules, fingerprinting, comprehensive assessment of activity, molecular docking

CLC Number: 

  • R285