Shandong Science ›› 2025, Vol. 38 ›› Issue (5): 10-21.doi: 10.3976/j.issn.1002-4026.20240113
• Traditional Chinese Medicine and Natural Active Products • Previous Articles Next Articles
WANG Haiyan1(), YU Ping1, WANG Xin1, YU Hongxia2, ZHOU Jie1, FANG Lei1,3,*(
)
Received:
2024-09-25
Revised:
2025-01-16
Published:
2025-10-20
Online:
2025-10-11
CLC Number:
WANG Haiyan, YU Ping, WANG Xin, YU Hongxia, ZHOU Jie, FANG Lei. Optimization of enzyme-water continuous extraction of ginsenosides from fibrous roots of American ginseng using response surface methodology[J].Shandong Science, 2025, 38(5): 10-21.
Table 4
Design and results of response surface tests"
实验号 | A液料比 | B提取温度 | C提取时间 | Y总皂苷提取率/% |
---|---|---|---|---|
1 | -1 | -1 | 0 | 12.01 |
2 | 1 | -1 | 0 | 12.45 |
3 | -1 | 1 | 0 | 14.16 |
4 | 1 | 1 | 0 | 14.17 |
5 | -1 | 0 | -1 | 13.16 |
6 | 1 | 0 | -1 | 12.51 |
7 | -1 | 0 | 1 | 12.67 |
8 | 1 | 0 | 1 | 13.89 |
9 | 0 | -1 | -1 | 12.51 |
10 | 0 | 1 | -1 | 13.61 |
11 | 0 | -1 | 1 | 12.81 |
12 | 0 | 1 | 1 | 14.43 |
13 | 0 | 0 | 0 | 14.56 |
14 | 0 | 0 | 0 | 14.41 |
15 | 0 | 0 | 0 | 14.21 |
16 | 0 | 0 | 0 | 14.49 |
17 | 0 | 0 | 0 | 14.32 |
Table 5
The results of variance analysis"
来源 | 离均差平方和 | 自由度 | 均方 | F | P | 显著性 |
---|---|---|---|---|---|---|
模型 | 12.290 | 9 | 1.370 | 38.24 | <0.000 1 | ** |
A | 0.130 | 1 | 0.130 | 3.64 | 0.098 0 | |
B | 5.430 | 1 | 5.430 | 152.00 | <0.000 1 | ** |
C | 0.510 | 1 | 0.510 | 14.14 | 0.007 1 | ** |
AB | 0.046 | 1 | 0.046 | 1.29 | 0.292 7 | |
AC | 0.870 | 1 | 0.870 | 24.48 | 0.001 7 | ** |
BC | 0.068 | 1 | 0.068 | 1.89 | 0.211 3 | |
A2 | 2.320 | 1 | 2.320 | 64.82 | <0.000 1 | ** |
B2 | 0.890 | 1 | 0.890 | 24.84 | 0.001 6 | ** |
C2 | 1.510 | 1 | 1.510 | 42.30 | 0.000 3 | ** |
残差 | 0.250 | 7 | 0.036 | |||
失拟项 | 0.170 | 3 | 0.058 | 3.04 | 0.155 6 | |
纯误差 | 0.076 | 4 | 0.019 | |||
总和 | 12.540 | 16 |
Table 6
Design and results of response surface tests"
实验号 | A液料比 | B提取温度 | C提取时间 | Y总皂苷提取率/% |
---|---|---|---|---|
1 | -1 | -1 | 0 | 14.91 |
2 | 1 | -1 | 0 | 15.43 |
3 | -1 | 1 | 0 | 15.48 |
4 | 1 | 1 | 0 | 15.75 |
5 | -1 | 0 | -1 | 14.82 |
6 | 1 | 0 | -1 | 15.53 |
7 | -1 | 0 | 1 | 15.47 |
8 | 1 | 0 | 1 | 15.79 |
9 | 0 | -1 | -1 | 14.94 |
10 | 0 | 1 | -1 | 15.58 |
11 | 0 | -1 | 1 | 15.48 |
12 | 0 | 1 | 1 | 15.81 |
13 | 0 | 0 | 0 | 15.68 |
14 | 0 | 0 | 0 | 15.57 |
15 | 0 | 0 | 0 | 15.63 |
16 | 0 | 0 | 0 | 15.59 |
17 | 0 | 0 | 0 | 15.63 |
Table 7
The results of variance analysis"
来源 | 离均差平方和 | 自由度 | 均方 | F | P | 显著性 |
---|---|---|---|---|---|---|
模型 | 1.430 | 9 | 0.160 | 63.12 | <0.0001 | ** |
A | 0.410 | 1 | 0.410 | 164.21 | <0.000 1 | ** |
B | 0.430 | 1 | 0.430 | 175.51 | <0.000 1 | ** |
C | 0.350 | 1 | 0.350 | 139.92 | <0.000 1 | ** |
AB | 0.016 | 1 | 0.016 | 6.20 | 0.041 6 | * |
AC | 0.038 | 1 | 0.038 | 15.08 | 0.006 0 | ** |
BC | 0.024 | 1 | 0.024 | 9.53 | 0.017 6 | * |
A2 | 0.081 | 1 | 0.081 | 32.15 | 0.000 8 | ** |
B2 | 0.033 | 1 | 0.033 | 13.15 | 0.008 4 | ** |
C2 | 0.026 | 1 | 0.026 | 10.36 | 0.014 7 | * |
残差 | 0.018 | 7 | 2.521×10-3 | |||
失拟项 | 0.010 | 3 | 3.483×10-3 | 1.94 | 0.265 7 | |
纯误差 | 7.20×10-3 | 4 | 1.800×10-3 | |||
总和 | 1.450 | 16 |
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