基于网络药理学和体外实验拓扑分析三七总皂苷治疗痛风性关节炎的作用机制

doi:10.3976/j.issn.1002-4026.20240121

Abstract

Abstract:

In this study，we analyzed the active components and mechanism of Panax notoginseng saponin （PNS） in treatment of gouty arthritis （GA） through network pharmacology，molecular docking，and cellular experiments. An in vitro hyperuricemia cell model was established using adenosine and xanthine oxidase to induce HK-2 cells. Moreover，the uric acid-lowering activity of PNS at different doses was assessed. Network pharmacology was used to explore the potential mechanism of PNS in the treatment of GA，and key targets were validated using molecular docking. Results revealed that PNS could inhibit the release of uric acid from renal tubular epithelial cells that was induced by adenosine and xanthine oxidase. In addition，15 key targets related to GA intervention were identified from PNS. Results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses suggested that PNS may exert therapeutic effects on GA by regulating multiple signaling pathways，such as JAK2-STAT3，AGE-RAGE，and calcium signaling. The molecular docking results showed that the binding energies of each active component with key targets such as STAT3，PTAFR，and IL2 were all lower than -5 kcal/mol，indicating good affinity，which can be used as potential therapeutic targets. This study provides a reference for the use of PNS in the treatment of GA.

Key words: Panax notoginseng saponin, gouty arthritis, network pharmacology, molecular docking, mechanism

CLC Number:

R288

GUO Yingjian, YU Jinqian, DONG Hongjing, WANG Chuangchuang, ZHONG Kai, WANG Xiao. Topological analysis reveals mechanism of Panax notoginseng saponin in the treatment of gouty arthritis based on network pharmacology and in vitro experiments[J].Shandong Science, 2025, 38(5): 40-48.

Figures/Tables 4

References 31

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活性成分	与靶点蛋白结合能/（kcal·mol^-1）
活性成分	STAT3	PTAFR	NR2F2	PTPN2	FGF2	IL2
三七总皂苷R1	-7.7	-7.4	-7.8	-7.5	-6.9	-6.9
人参皂苷Rg1	-7.2	-7.1	-7.3	-6.3	-6.3	-6.2
人参皂苷Re	-8.1	-7.5	-7.7	-8.0	-6.9	-5.9
人参皂苷Rb1	-7.4	-8.5	-7.0	-7.0	-6.9	-5.7
人参皂苷Rd	-7.0	-7.8	-7.3	-7.5	-6.8	-5.8

Topological analysis reveals mechanism of Panax notoginseng saponin in the treatment of gouty arthritis based on network pharmacology and in vitro experiments

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