新加苁蓉菟丝汤通过线粒体自噬途径促卵泡发育的分子机制

doi:10.3976/j.issn.1002-4026.20240015

Abstract

Abstract:

Using light chromatography-mass spectrometry (LC-MS), network pharmacology, and animal experiments, we explored the molecular mechanism by which Xinjia Cistanche Tusi Decoction (XJCRTST) promotes follicular development through granulosa cell mitophagy. LC-MS and network pharmacology were used to screen for the main components of XJCRTST and its therapeutic targets and pathways related to hypoovarianism. Female Sprague-Dawley rats were divided into five groups: model group, western medicine group, and XJCRTST high dose, medium dose, and low dose groups. The morphological changes of the ovary were observed through hematoxylin and eosin staining, and the structure and morphology of mitochondria and mitophagosomes in ovarian granulosa cells were observed through transmission electron microscopy. Two hundred and fifty-two compounds were identified in XJCRTST, with 42 potential targets for treating hypoovarianism, and the Gene Ontology enrichment mainly involved biological process (1 168 items), cell composition(45 items), and molecular function(59 items). KEGG pathway enrichment showed that the pathways involved were AGE-RAGE, Prolactin and TNF signaling pathways. Three target genes Parkin/PINK1, HIF1A and MAPK8 were enriched in the Mitophagy-animal process.Animal experiments showed that the number of follicles increased and the atretic follicles decreased in the Chinese medicine treatment group. Electron microscopy found that mitochondrial swelling and mitophagy decreased in the Chinese medicine group. XJCRTST may play a therapeutic role in treating hypoovarianism by regulating mitochondrial function and mitophagy-related pathways in ovarian granulosa cells.

Key words: Xinjia Cistanche Tusi Decoction, ovarian hypoplasia, network pharmacology, LC-MS, mitochondria

CLC Number:

R282

YAN Jin, LI Fei, WU Keming, YANG Haobo. The molecular mechanisms by which Xinjia Cistanche Tusi Decoction promotes follicular development through mitophagy[J].Shandong Science, 2024, 37(5): 25-34.

Figures/Tables 9

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References 15

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靶点	ASPL	度值	靶点	ASPL	度值	靶点	ASPL	度值
AKT1	1.46153846	21	MCL1	1.8974359	12	DRD1	2.25641026	7
ESR1	1.51282051	19	IL2	1.92307692	12	PINK1	1.97435897	6
COMT	1.58974359	19	CASP8	1.92307692	12	OPRM1	2.00000000	6
TNF	1.71794872	19	DRD2	1.79487179	11	F2	2.05128205	6
SLC6A3	1.56410256	18	CHRNA7	1.82051282	10	NR1H4	2.1025641	6
VEGFA	1.82051282	15	HTR2A	2.12820513	10	PTGS1	2.17948718	5
ESR2	1.64102564	14	CYP1A2	1.84615385	9	AKR1C3	2.23076923	5
HIF1A	1.82051282	14	KDR	1.87179487	9	ALOX5	2.25641026	4
MAPK14	1.87179487	13	CYP1A1	1.84615385	8	SHBG	2.25641026	4
STAT3	1.8974359	13	CYP1B1	1.92307692	8	OPRD1	2.30769231	4
MAOA	1.92307692	13	PARK2	1.92307692	8	ADRA2C	2.35897436	4
MAOB	1.97435897	13	HTR2C	2.07692308	8	ADA	2.56410256	2
CHRNA4	2.02564103	13	HTR1A	2.05128205	7
MAPK8	1.87179487	12	DRD5	2.25641026	7

The molecular mechanisms by which Xinjia Cistanche Tusi Decoction promotes follicular development through mitophagy

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