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    20 August 2018 Volume 31 Issue 4
      
    Oceanographic Science, Technology and Equipment
    Technology of hyperspectral push broom image mosaicking based on ENVI redevelopment
    GAI Ying-ying,GAI Zhi-gang,YU Ding-feng,LIU En-xiao,LI Hui,QIN Sheng-guang
    SHANDONG SCIENCE. 2018, 31(4):  1-7.  doi:10.3976/j.issn.1002-4026.2018.04.001
    Abstract ( )   PDF (4847KB) ( )  
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    As the narrow stripes obtained by the pushbroom imaging spectrometer need to be geometrically corrected before mosaicking into a spatial twodimensional image, a technique of hyperspectral pushbroom image mosaicking based on ENVI redevelopment was proposed in this paper. A relationship of points on the images between tilting state and orthographic state of the spectrometer was established based on homography mapping, and image distortion caused by the
    variation of flight posture was corrected. GPS data was combined to correct the stripe overlap due to the change of flight speed and the corrected stripes were mosaicked together. Technical integration was carried out on the ENVI redevelopment platform, realizing the automatic calibration and mosaicking of hyperspectral push broom stripes. Experiments were carried out in Baoding suburbs of Hebei province, and it showed that the mosaicking result obtained by proposed method was close to that obtained by spectrometer software. The coordinate differences of latitude and longitude were both within 1 m, and the root mean square error was about 0.738 9, which could meet the geographical accuracy requirements of general hyperspectral remote sensing application.
    Research on intelligent PID control of ship course based on BP neural network
    LI Xiao-feng,YU Hui-bin
    SHANDONG SCIENCE. 2018, 31(4):  8-14.  doi:10.3976/j.issn.1002-4026.2018.04.002
    Abstract ( )   PDF (1327KB) ( )  
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    According to the nonlinear characteristics of ship course control, and taking the first-order KT model of ship course motion as the research object, the selftuning PID algorithm course controller based on BP neural network was designed. Combining the traditional PID and the BP neural network, the controlled object was identified by the BP neural network, the PID control parameters were given, and the PID control algorithm was used to control and optimize the
    convergence speed. Based on a true ferry′s characteristic parameters, the ship′s course motion control system model was established using MATLAB/Simulink simulation software. The simulation results show that the design has small overshoot, good robustness, capable to work steadily for a long time, and almost no steadystate error. The practicability of the control algorithm and the superiority of the PID control system were verified.
    Analysis of the influence of wind direction resolution and zero position error on ship real wind calculation error
    LI Zhi-qian, GONG Yong-xiang,QI Sui-ping, HU Tong, YU Hong-bo, WANG Dong-ming
    SHANDONG SCIENCE. 2018, 31(4):  15-19.  doi:10.3976/j.issn.1002-4026.2018.04.003
    Abstract ( )   PDF (4421KB) ( )  
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    The wind sensors installed on the ship’s mast can measure the relative wind directly when voyaging. However, only by using the vector algorithm combined with the ship’s speed and heading can we calculate the true wind. Through quantitative analysis of the influence of the wind sensor’s zero installation error on true wind calculation, the error transfer model of the wind sensor’s direction resolution and zero installation error was established in this paper. Analysis
    shows that the wind sensor’s zero installation accuracy has important influence on the true wind calculating results, in some cases it can be up to an error of 30%. In order to improve the accuracy of the true wind calculating, we must strictly control the zero installation precision of the sensor.
    Traditional Chinese medicine and natural active products
    Determination of chemical components in flower buds and leaves of Lonicera japonica in Tibet area by HPLC-DAD
    LIU Qian,ZHANG Min-min,LI Sheng-bo,LIANG Yan,LIU Wei,CHENG Su-pan,WANG Xiao,GENG Yan-ling,ZHAO Heng-qiang
    SHANDONG SCIENCE. 2018, 31(4):  20-25.  doi:10.3976/j.issn.1002-4026.2018.04.004
    Abstract ( )   PDF (1115KB) ( )  
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    The active components of flower buds and leaves of Lonicera japonica “AT1”in Tibet area were analyzed to provide data support for their quality evaluation and exploitation. High performance liquid chromatography diode array detector (HPLC-DAD) was used to analyze 8 batches of Lonicera japonica Thunb. Flower buds and leaves in Tibet. The 8 main compounds of chlorogenic acid, luteoloside, loganin acid, cryptochlorogenic acid, loganin determination, Secoxyloganin,
    rutin, and isochlorogenic acid C content were measured, respectively. The buds were 3.85%, 0.15%,0.18%, 0.15%, 0.11%, 0.11%, 0.17% and 0.11%, and leaves: 3.58%, 0.79%,0.40%, 0.08%, 0.07%, 0.24%, 0.18% and 0.05%. This study provides a method and data support for the rapid detection of
    chemical constituents and further exploitation of the fine species of Lonicera japonica in Tibet.
    Quantitative 1H NMR based content determination of ginsenoside Rd
    YAN Hui-jiao, WANG Zhi-wei,LIN Yun-liang, GAO Hong-mei, LI Feng, WANG Xiao, GENG Yan-ling
    SHANDONG SCIENCE. 2018, 31(4):  26-30.  doi:10.3976/j.issn.1002-4026.2018.04.005
    Abstract ( )   PDF (2165KB) ( )  
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    To establish an accurate and rapid method for content determination of ginsenoside Rd with quantitative 1H NMR. Benzoic acid served as internal standard, DMSO-d6 and D2O (9:1, V/V) as solution.1H NMR spectra of the mixture of ginsenoside Rd and benzoic acid were acquired with spectrometer frequency 400.13 MHz scanning 32 times, relaxation delay time 4 s. Ginsenoside Rd proton peak at δ 5.07 and benzoic acid signal δ 7.94 served as quantification peaks. Linear regression equation of correlation coefficient of 0.997 8, RSD of precision were less than 0.65%, the average recoveries were in the range of 98.69% to 102.54% with RSD less than 1.92%. The contents of 3 batches of ginsenoside Rd were 98.87%, 98.79% and 98.92%. Experimental results show that the concentration of quantitative 1H NMR determined ginsenoside Rd is slightly lower than that by HPLC without reference substance. The method is simple, accurate, and has good application value, can be used for the content determination and quality control of ginsenoside Rd.

    Comparative study on the active constituents of Salvia miltiorrhiza in different strains
    LIANG Cong-lian, ZHANG Yong-qing, LI Jia, LIU Qian, PU Gao-bin
    SHANDONG SCIENCE. 2018, 31(4):  31-38.  doi:10.3976/j.issn.1002-4026.2018.04.006
    Abstract ( )   PDF (2680KB) ( )  
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    To compare the differences of effective components of Salvia miltiorrhiza from different strains, the contents of 4 fat-soluble components including tanshinone IIA and the contents of 3 water-soluble components including salvianolic acid B were measured by HPLC, and the data were analyzed by SPSS software. By comparison of the two major kinds of ingredients, it was found that the contents of fatsoluble components in 3, 7 strains were relatively higher, and the contents of watersoluble components in 1, 9, 10 strains were higher, so we could consider to cultivate 3,7 strains for the raw materials of the extraction of fatsoluble component, while cultivating 1,9,10 strains for the extraction of watersoluble components. The significance analysis results showed that there were significant differences in the main watersoluble and fatsoluble components of 10 different strains of S. miltiorrhiza. This study can provide a basis for the selection of good germplasm of S. miltiorrhiza.

    Research progress on quality assessment of Panacis Quinquefolii Radix
    CHENG Ke-qing, WANG Chong-xu, XU Tong, LIN Ying, ZHOU Jie, DONG Peng, LI Li-ming, YU Jing-ping
    SHANDONG SCIENCE. 2018, 31(4):  39-43.  doi:10.3976/j.issn.1002-4026.2018.04.007
    Abstract ( )   PDF (1030KB) ( )  
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    Based on the systematic analysis of the literatures on the quality evaluation of Panacis Quinquefolii Radix, the main factors affecting the quality of Panacis Quinquefolii Radix were summarized in terms of the place of origin, harvesting period and drying methods. It is considered that the key problem in the quality evaluation of Panacis Quinquefolii Radix is that the evaluation index is too onesided. The study of future quality evaluation should be based on the integration of effective ingredient, pharmacological action and traditional Chinese medicine effect, focusing on the theory of traditional Chinese medicine and the overall quality evaluation technology. Finally, it is pointed out that the study of medicinal quality should guide the screening of the origin of medicinal plants, regulate the growth and serve the specification of medicine market, so as to promote the modernization process of traditional Chinese medicine.

    New Materials
    Simulation of casting process for large aluminum alloy impeller based on MAGMASOFT
    SUN De-zhi,WANG Gui-qing,TIAN Chang-wen
    SHANDONG SCIENCE. 2018, 31(4):  44-49.  doi:10.3976/j.issn.1002-4026.2018.04.008
    Abstract ( )   PDF (10045KB) ( )  
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    A sand casting process scheme for large aluminum alloy impeller casting was designed in this paper. By using the casting simulation software MAGMASOFT, the filling process, the distribution of the gas defects and the temperature field distribution of the casting were simulated. According to the analysis, the main reason for the defects of the castings was the unreasonable design of the riser at the mouth ring. It is proposed to add insulation sleeve to the riser at the mouth ring to increase the feeding effect of the riser. The simulation results show that the problems of shrinkage porosity and shrinkage tendency of castings are effectively solved after optimizing the process.

    Effect of aramidshort fiber and aramid pulp on the structure and properties of rubber composites
    CHEN Chun-hua, XU Zhan, SHEN Bin, XIN Zhen-xiang
    SHANDONG SCIENCE. 2018, 31(4):  50-58.  doi:10.3976/j.issn.1002-4026.2018.04.009
    Abstract ( )   PDF (13129KB) ( )  
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    In this paper, the microstructure characterization and thermal weight loss analysis of aramid short fiber and aramid pulp were carried out. The effects of aramid short fiber/aramid pulp on the basic physical properties, dynamic mechanical properties and dynamic stiffness of the rubber composites were systematically studied. The results show that, aramid fiber has a giant fibrous model, and Kevlar pulp has microfiber model. The degradation of aramid staple fibers at 500 ℃ was basically complete at about 650 ℃. The aramid pulp was degraded at about 350 ℃ and degraded quickly at about 500 ℃, and the degradation was basically complete at about 650 ℃. The smaller the ratio of aramid short fiber/aramid pulp is, the greater the tear strength and elongation, the better the flexural properties of the rubber composite, but the smaller the dynamic stiffness. The dynamic mechanical properties show that when the ratio of aramid fiber and aramid pulp is 10:10, it has relatively higher storage modulus, lower loss modulus and loss factor, and better dynamic fatigue performance.

    Sensibility study on the corrosion of N80 steel during the process of flue gas flooding
    ZHOU Ying-mei
    SHANDONG SCIENCE. 2018, 31(4):  59-64.  doi:10.3976/j.issn.1002-4026.2018.04.010
    Abstract ( )   PDF (1260KB) ( )  
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    As the technology of flue gas injection is increasingly used,severe corrosion arises in the flue gas injection wellbore. This paper conducted a study on the corrosion of N80 steel in high temperature and high pressure flue gas and used the grey correlation analysis method to analyze sensibility of N80 steel corrosion. The results show that corrosion rate rises with ascension of the temperature when temperature is lower than 60 ℃,and reaches the maximum at 60 ℃;With the pressure increases, the corrosion rate increases. With increasing in flow rate and oxygen content,the corrosion rate increases;With the increase of SO2 content,the corrosion rate shows a trend of increasing first and then rising. The order of the influence of each factor on the corrosion rate is temperature, oxygen content, pressure, SO2 content, NaCl content, velocity and NaHCO3 content.

    Energy and Power
    Experimental investigation of boiling incipience in micro pin fin heat sinks
    KONG Ling-jian, LIU Zhi-gang, JI Can, JIANG Ya-ke
    SHANDONG SCIENCE. 2018, 31(4):  65-71.  doi:10.3976/j.issn.1002-4026.2018.04.011
    Abstract ( )   PDF (5370KB) ( )  
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    An experiment study has been conducted to investigate the onset of nucleate boiling (ONB) characteristics of deionized water in circular, diamond and elliptic micro pin fin heat sinks. The experiments were carried out in a range of mass flux from 292.8 to 412.2 kg/(m2·s), inlet temperature from 50.6 to 81.5 °C, heat flux from 10.1 to 87.1 W/cm2. The wall temperature and pressure drop distributions of the micro pin fin heat sinks were analyzed from singlephase forced convection to twophase flow boiling heat transfer. The experiment results indicate that the ONB can be identified as the point at which deviations from singlephase trend is shown as a sudden change in temperature and pressure drop versus the heat flux. A parametric study was explored to investigate the effects of the experimental parameters on ONB. The heat flux at the ONB increases with an increase in mass flux. However, raising the inlet temperature can cause a reduction on the heat flux at the ONB. Besides, the heat flux at the ONB in circular, diamond and elliptic micro pin fin heat sink decreases successively under the same experimental condition.

    Effect of DBA、SDBS on phase separation and coking granularity during thermal reaction
    ZHOU Ying-mei
    SHANDONG SCIENCE. 2018, 31(4):  72-78.  doi:10.3976/j.issn.1002-4026.2018.04.012
    Abstract ( )   PDF (5680KB) ( )  
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    In this paper, the phase separation and coke granularity of Liaohe atmospheric residuum (LHAR) and Karamay atmospheric residuum (KLAR) in the coking reaction were investigated. The effects of additives on the phase process and coke particle size were also investigated. The results showed that when sodium dodecyl benzene sulfonate (SDBS) and 4-Dodecylbenzenesulfonic acid (DBA) were added in LHAR,it could obviously change the phase separation process during the process of thermal reaction, the capacity of coking was increased and the size of coke was obviously reduced. The coke yield of KLAR in the coking reaction was decreased dramatically due to the addition of DBA. DBA could interact with the polar functional groups of asphaltene colloid in the process of thermal reaction. Therefore, the aggregation of asphaltene was inhibited and the phase separation of the system was delayed. The coke was smaller and the number of coke centers increased with the addition of DBA, So the coke size was reduced greatly when the total coke amount was similar.

    Biosensors
    A study of optimal fermentation conditions for phytase production by engineered strain
    YANG Jun-hui, GONG Wei-li, YANG Yan, MENG Qing-jun, SHI Jian-guo
    SHANDONG SCIENCE. 2018, 31(4):  79-84.  doi:10.3976/j.issn.1002-4026.2018.04.013
    Abstract ( )   PDF (4952KB) ( )  
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    To study the factors affecting phytase production by engineered Pichia pastoris, we explored the optimal fermentation conditions, such as methanolfedbatch concentration or induction time, with shake flask fermentation. The results demonstrated that a series of factors including residual glycerol, methanol concentration and induction time had significant effects on phytase production. Residual glycerol could delay the phytase secretion, 1.5% methanol had the best effect of induction with enzyme activity reaching about 1 100 U/mL in the second day, while low concentration of methanol could only slowly induce enzyme accumulation, and high concentration of methanol was harmful to engineered strain. This study lays a foundation for further optimization of high density fermentation conditions.

    Influence of the wet cell weight before induction on secretive expression of phytase in Pichia pastoris
    ZHAO Kai, GE Jing-hua, WANG Hai
    SHANDONG SCIENCE. 2018, 31(4):  85-88.  doi:10.3976/j.issn.1002-4026.2018.04.014
    Abstract ( )   PDF (1285KB) ( )  
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    In order to increase the expression of phytase in Pichia pastoris, the fermentation conditions of phytaseproducing recombinant Pichia pastoris in 50 L fermentation tank were optimized. The results show that the strain wet weight at 300 g/L by feeding glucose, before addition of methanol, got the phytase activity 21 666 U/mL, 12.9% higher than the control by the end of fermentation, which is most favorable for the production of phytase by fermentation.

    Tranfic and Transportation
    Research on recharging infrastructure planning for electric vehicles based on battery swapping
    GU Teng-fei, ZHANG Yong
    SHANDONG SCIENCE. 2018, 31(4):  89-99.  doi:10.3976/j.issn.1002-4026.2018.04.015
    Abstract ( )   PDF (2493KB) ( )  
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    Electric vehicles have drawbacks such as long charging time and limited driving range. Based on the charging method of battery swapping, the battery swapping stations and batteries configuration of electric vehicles were investigated in this paper. The processes of battery swapping and charging at swapping stations along a long corridor were described with Birth and Death processes theory, and then a stochastic model for the operation of the swapping station was established.Furthermore, taking the electric vehicle’s probability of swapping a fullycharged battery successfully as the constrain, recharging infrastructure planning models were established with the goal of minimum construction cost, and a model solving algorithm was proposed based on the Lagrange method. Research results show that the proposed model can not only calculate the successful probability of battery swapping, but also analyze the effect of the probability of swapping successfully and the progress of the charging technology, realizing the optimal swapping stations and batteries configuration with minimum construction cost under the constraint of the successful probability of battery swapping.

    Application of pedestrian simulation in passenger transportation evaluation of transfer stations in urban rail transit
    CHEN Ming-dian,ZHENG Xuan-chuan,GAO Guo-fei
    SHANDONG SCIENCE. 2018, 31(4):  100-109.  doi:10.3976/j.issn.1002-4026.2018.04.016
    Abstract ( )   PDF (8561KB) ( )  
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    In this paper, the Legion pedestrian simulation software was used to evaluate and analyze the passenger transport of Qingniandajie Station in Shenyang Metro during the morning peak. The parameters of the simulation model were calibrated through field investigations and statistical analysis of the original ticket card data. Based on the Fruin service level evaluation standard, the service level of whole station and key areas, and the rationality of the station’s flow lines were evaluated and analyzed. The evaluation results show that the overall service level of Qingniandajie Station during the morning peak is relatively low, and the bottleneck areas of passenger traffic are concentrated in the four groups of transfer stairs and escalators and platform areas. Finally, through analysis of the causes of the station congestion, the improvement measures for relieving the station congestion were put forward, which provide references for renovations of the stations and optimization of passenger transport organization.

    Research on spatiotemporal data organization and visualization of navigation in channel
    BI Jin-qiang, XIN Quan-bo, XU Jia-shuai, SHANG Dong-fang, KOU Li-ying
    SHANDONG SCIENCE. 2018, 31(4):  110-117.  doi:10.3976/j.issn.1002-4026.2018.04.017
    Abstract ( )   PDF (6184KB) ( )  
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    In order to solve the semantic independence and heterogeneity of GIS data resources in channel, the paper analyzed the structure and management mechanism of metadata and proposed a distributed spatial data organization storage method based on XML. According to the level and relevance of the geographical location distribution of the main elements of the channel, the method established a spatiotemporal integrated data storage model with spatial reference. On this foundation, the paper realized visualization of spatial and temporal data, which applied such as spatiotemporal GIS multidimensional modeling technology, terrain scene construction technology, discrete observation data interpolation technology, etc. Taking the upper reaches of the Yangtze River as an example, multisource heterogeneous data fusion organization was realized and an application system was developed based on threetier technical architecture, which was applied to ship navigation and auxiliary decisionmaking successfully. Application results show that the study can effectively solve the difficulties of data organization and visualization in complex waterway environment, and provide security guarantee and support for ship navigation.

    Short term prediction of road travel time based on an ensemble algorithm
    JIANG Yi-yue, DONG Shu-qian, ZHOU Shu-min
    SHANDONG SCIENCE. 2018, 31(4):  118-125.  doi:10.3976/j.issn.1002-4026.2018.04.018
    Abstract ( )   PDF (2379KB) ( )  
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    In order to better solve the short term prediction problem of travel time on links, a tree based ensemble method was proposed and improved. First, a more robust GBDT was established to reduce the disturbance caused by bursts aiming at the strong upheavals of traffic in a small time scale. Then, the RF and GBDT were fused and a new method for RF-GBDT was proposed to overcome the problem of bias-variance dilemma. In addition, various relevant variables derived from historical travel time data were considered to improve the interpretability of the model. The results of predictions show that compared with the single RF or GBDT, the RF-GBDT method is preferable in the accuracy and the stability of algorithms.

    Other Research Article
    Histidine induced DNA charge inversion and condensation
    LU Jie-ping, WANG Yan-wei, YANG Guang-can
    SHANDONG SCIENCE. 2018, 31(4):  126-132.  doi:10.3976/j.issn.1002-4026.2018.04.019
    Abstract ( )   PDF (3545KB) ( )  
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    Taking histidine as the research object, it was observed by dynamic light scattering that the electrophoretic mobility changes from negative to positive, and reversal of DNA charge when the histidine concentration was 1 mg/mL. At the same time, the following phenomena could be observed by atomic force microscopy: as the concentration of histidine increased, DNA molecules gradually piled up and folded up to a sphere at a histidine concentration of 1 mg/mL, which indicated that histidine could lead to DNA condensation and charge reversal. Since the isoelectric point of histidine is 7.59, it is believed that the protonation and deprotonation of the histidine side chain play a role in the regulation of its charged state. As a basic genetic material in the organism, DNA affects the growth and development of organisms, which is also closely related to abnormal activities such as cell cancer and mutation. In cells, histones are significant part of the DNA chromosomes and regulate DNA condensation in the process of DNA transcription and replication. Histidine is the main component of histones. The study of the effect of histidine on DNA condensation and related charge reversal processes is of fundamental importance for understanding the regulation of histones.

    Research on control system of legtrack complex hoisting robots based on wireless telecontrol technology
    WANG Ya-li, YAN Jiu-xiang, ZHANG Guo-hui, WEI Pan-pan
    SHANDONG SCIENCE. 2018, 31(4):  133-138.  doi:10.3976/j.issn.1002-4026.2018.04.020
    Abstract ( )   PDF (1697KB) ( )  
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    Using the wireless telecontrol technology and builtin control algorithm, a novel wireless intelligent telecontrol system of leg-track complex robots was constructed to complete the hoisting tasks in the narrow space or abominable road. The control system adopted a special motion controller as the core component for the control and the wireless telecontrol module as the interactive interface. By the builtin control algorithm and CAN bus technology, the hoisting tasks in different types of sites could be done while the engine speed and the vehicle balance was adjusted in real time according to the actual task requirements, the weight and the flatness of robot body. The control system was applied for fully automatic remotecontrol operation, such as engine start or stop, posture adjustment, adjusting the balance of the vehicle body, robot motion control, the output power optimization et al. The system improves the convenience of the operation of the lifting robot and the reliability in the working process. The hoisting task can be finished while the operator is far away from the robot.