氢核磁定量分析技术测定人参皂苷Rd对照品含量的研究

doi:10.3976/j.issn.1002-4026.2018.04.005

Abstract

Abstract:

To establish an accurate and rapid method for content determination of ginsenoside Rd with quantitative ¹H NMR. Benzoic acid served as internal standard, DMSO-d6 and D₂O (9:1, V/V) as solution.¹H NMR spectra of the mixture of ginsenoside Rd and benzoic acid were acquired with spectrometer frequency 400.13 MHz scanning 32 times, relaxation delay time 4 s. Ginsenoside Rd proton peak at δ 5.07 and benzoic acid signal δ 7.94 served as quantification peaks. Linear regression equation of correlation coefficient of 0.997 8, RSD of precision were less than 0.65%, the average recoveries were in the range of 98.69% to 102.54% with RSD less than 1.92%. The contents of 3 batches of ginsenoside Rd were 98.87%, 98.79% and 98.92%. Experimental results show that the concentration of quantitative ¹H NMR determined ginsenoside Rd is slightly lower than that by HPLC without reference substance. The method is simple, accurate, and has good application value, can be used for the content determination and quality control of ginsenoside Rd.

Key words: content determination, quantitative ¹H NMR, ginsenoside Rd

CLC Number:

R284.1

YAN Hui-jiao, WANG Zhi-wei,LIN Yun-liang, GAO Hong-mei, LI Feng, WANG Xiao, GENG Yan-ling. Quantitative ¹H NMR based content determination of ginsenoside Rd[J].SHANDONG SCIENCE, 2018, 31(4): 26-30.

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Quantitative ¹H NMR based content determination of ginsenoside Rd