醋红大戟的质量标准研究

doi:10.3976/j.issn.1002-4026.2023.04.003

Abstract

Abstract:

The study aimed to conduct a comprehensive study on the quality requirements for Knoxiae Radix processed with vinegar and to offer technical support for the formulation of the standard of Shandong Province Chinese herbal medicine processing specification. On the basis of the quality standard of vinegar-processed Knoxiae Radix in Shandong Province Chinese herbal medicine processing specification (2012), 29 batches of Knoxiae Radix samples were collected. Following vinegar processing, the parameters including characteristics, moisture, total ash, acid insoluble ash, alcohol soluble extract content, microscopic, and thin layer and content determination were systematically studied and analyzed and the relevant limits were formulated. The project was established on the basis of the current quality standard, in which the microscopic identification, and the lucidin content determination were added, and the extractum item were revised when compared with current quality standard. This study established the Knoxiae Radix quality control index after vinegar processing. The established method of quality control is simple, reproducible, and accurate which can be used for quality control of vinegar-processed Knoxiae Radix.

Key words: vinegar-processed Knoxiae Radix, identification, quality standard, lucidin, content determination

CLC Number:

R284.1

CHEN Xi, GAO Yan, WANG Yanghai, JIA Mingqian, ZHANG Yue, MA Lulan, ZHAO Bonian. Study on the quality standard of vinegar-processed Knoxiae Radix[J].Shandong Science, 2023, 36(4): 18-28.

Figures/Tables 13

Fig.1

Fig.2

Table 1

Fig.3

Table 2

Fig.4

Table 3

Results of precision test"

编号	峰面积	平均峰面积	$δ R S D$ /%
1	16 389	16 353	0.29
2	16 395
3	16 397
4	16 284
5	16 336
6	16 316

Table 3

Table 4

Results of stability test"

进样时间/ h	峰面积	平均峰面积	$δ R S D$ /%
0	16 496	16 396	0.45
2	16 457
4	16 407
8	16 385
12	16 296
24	16 337

Table 4

Table 5

Results of repetitive test"

编号	峰面积值	均值	质量分数/(mg·g^-1)	平均质量分数/(mg·g^-1)	$δ R S D$ /%
1-1	16 350	16 364	2.50	2.46	1.01
1-2	16 377	16 364	2.50
2-1	16 264	16 280	2.48
2-2	16 297	16 280	2.48
3-1	16 138	16 149	2.46
3-2	16 160	16 149	2.46
4-1	16 012	16 020	2.44
4-2	16 028	16 020	2.44
5-1	15 836	15 880	2.43
5-2	15 924	15 880	2.43
6-1	16 008	16 040	2.45
6-2	16 072	16 040	2.45

Table 5

Table 6

Results of recovery test of lucidine"

编号	称样量/g	样品中含量/mg	测得量/mg	对照品加入量/mg	回收率/%	平均回收率/%	$δ R S D$ /%
1	0.502 3	0.331 5	0.636 0	0.312 5	97.44	99.16	1.07
2	0.501 6	0.331 0	0.642 4	0.312 5	99.65
3	0.504 2	0.332 7	0.646 6	0.312 5	100.45
4	0.503 3	0.332 1	0.644 2	0.312 5	99.87
5	0.503 5	0.332 3	0.641 3	0.312 5	98.90
6	0.504 8	0.333 1	0.641 5	0.312 5	98.67

Table 6

Table 7

Table 8

Fig.5

References 18

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样品编号	w(水分)/%	w(总灰分)/%	w(酸不溶性灰分)/%	w(浸出物)/%
1	7.06	11.91	2.70	50.94
2	9.08	11.63	1.98	50.57
3	8.20	11.59	1.72	50.75
4	9.28	11.78	2.09	52.74
5	7.19	11.22	1.47	51.51
6	5.98	14.50	2.58	45.80
7	8.60	11.75	1.93	51.97
8	7.89	12.83	2.29	51.25
9	7.52	14.51	2.32	47.63
10	8.24	11.44	2.06	48.52

编号	进样量/μg	峰面积
1	0.25	1 071
2	0.62	3 369
3	1.25	7 309
4	1.88	11 328
5	2.50	15 496
6	3.75	23 459

序号	称样量/g	峰面积	芦西定质量分数/%	平均质量分数/%
1-1	1.009 5	11 531 048	0.184 7	0.184 5
1-2	1.001 4	11 420 657	0.184 4	0.184 5
2-1	1.008 3	3 040 587	0.048 7	0.048 7
2-2	1.007 5	3 033 430	0.048 7	0.048 7
3-1	1.002 4	7 133 074	0.115 0	0.112 4
3-2	1.009 8	6 853 125	0.109 7	0.112 4
4-1	1.012 0	6 030 045	0.096 3	0.093 9
4-2	1.004 4	5 685 088	0.091 5	0.093 9
5-1	1.017 0	7 014 051	0.111 5	0.112 9
5-2	1.014 9	7 172 049	0.114 2	0.112 9
6-1	1.015 9	4 176 313	0.066 5	0.066 6
6-2	1.000 5	4 131 090	0.066 7	0.066 6
7-1	1.009 4	4 289 685	0.068 7	0.068 0
7-2	1.002 1	4 167 083	0.067 2	0.068 0
8-1	1.000 1	3 937 391	0.063 6	0.064 1
8-2	1.000 5	3 999 106	0.064 6	0.064 1
9-1	1.004 2	6 380 008	0.102 7	0.103 6
9-2	1.013 0	6 544 643	0.104 4	0.103 6
10-1	1.003 5	8 044 776	0.129 6	0.bi131 2
10-2	1.015 4	8 347 371	0.132 9	0.bi131 2

序号	称样量/g	峰面积	芦西定质量分数/%	平均质量分数/%
1-1	1.002 9	9 957.756 5	0.160 9	0.159 5
1-2	1.007 5	9 838.266 0	0.158 2	0.159 5
2-1	1.004 3	5 076.464 5	0.081 9	0.080 9
2-2	1.009 9	4 980.033 0	0.079 9	0.080 9
3-1	1.007 6	15 973.488 5	0.256 8	0.255 8
3-2	1.009 8	15 877.234 0	0.254 7	0.255 8
4-1	1.008 6	6 719.530 0	0.107 9	0.108 1
4-2	1.007 0	6 731.740 0	0.108 3	0.108 1
5-1	1.008 2	5 262.974 0	0.084 6	0.085 5
5-2	1.004 9	5 355.437 0	0.086 3	0.085 5
6-1	1.001 9	19 341.067 0	0.312 7	0.314 7
6-2	1.002 9	19 608.138 0	0.316 7	0.314 7
7-1	1.009 3	7 994.255 0	0.128 3	0.128 0
7-2	1.004 7	7 922.574 5	0.127 7	0.128 0
8-1	1.109 6	4 326.914 0	0.063 2	0.063 4
8-2	1.111 9	4 365.194 0	0.063 6	0.063 4
9-1	1.003 6	4 146.123 0	0.066 9	0.067 9
9-2	1.003 2	4 259.750 5	0.068 8	0.067 9
10-1	1.013 8	10 058.182 5	0.160 7	0.161 2
10-2	1.013 7	10 110.278 5	0.161 6	0.161 2

Study on the quality standard of vinegar-processed Knoxiae Radix

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