草莓炭疽病拮抗木霉菌筛选、鉴定及功能评价

doi:10.3976/j.issn.1002-4026.2025027

Abstract

Abstract:

In response to the severe incidence and management challenges of strawberry anthracnose, this study targeted Colletotrichum siamense, the pathogen responsible for the disease.We used dual culture and sealed-plate assays to screen for the effective biocontrol Trichoderma strain HB 23422, which exhibited strong antagonistic activity against strawberry anthracnose,and was identified based on morphological characteristics and molecular biology as Trichoderma asperellum. On PDA plates, HB 23422 exhibited a strong inhibitory effect on Colletotrichum siamense CM9, with an inhibition rate of more than 74.55%. Vitro leaf experiments and pot experiments showed that the spore suspension of this strain could effectively reduce the incidence of strawberry anthracnose. The strain HB 23422 grows rapidly, produces abundant spores, and has excellent disease control efficacy, indicating its potential for development as microbial pesticide for environment friendly control of strawberry anthracnose.

Key words: Colletotrichum siamense, Trichoderma, separation and identification, inhibition rate

CLC Number:

S182

LIU Jianhua, JIANG Yanqing, LI Hongmei, YANG Jianwen, WEI Yanli, LIU Baojun, LI Jishun, HU Jindong. Screening,identification and functional evaluation of trichoderma strains antagonistic to strawberry anthracnose[J].Shandong Science, 2025, 38(6): 60-68.

Figures/Tables 10

Table 1

Fig.1

Fig.2

Fig.3

Fig.4

Table 2

Table 3

Fig.5

Table 4

Fig.6

References 27

[1]	宁志怨, 钱小强, 伊兴凯, 等. 氨基酸水溶肥对促进草莓产量、品质及生长特性的影响[J]. 浙江农业科学, 2020, 61(12): 2565-2567. DOI: 10.16178/j.issn.0528-9017.20201237.
[2]	王鸣谦, 薛莉, 赵珺, 等. 世界草莓生产及贸易现状[J]. 中国果树, 2021(2): 104-108. DOI: 10.16626/j.cnki.issn1000-8047.2021.02.025.
[3]	姜秋同, 张爽, 朱洪坤, 等. 草莓病害的发生与防治措施[J]. 现代农村科技, 2023(7): 49-50.
[4]	HIRAYAMA Y, ASANO S, OKAYAMA K, et al. Weeds as the potential inoculum source of Colletotrichum fructicola responsible for strawberry anthracnose in Nara, Japan[J]. Journal of General Plant Pathology, 2018, 84(1): 12-19. DOI: 10.1007/s10327-017-0753-4.
[5]	胡德玉, 钱春, 刘雪峰. 草莓炭疽病研究进展[J]. 中国蔬菜, 2014(12): 9-14.
[6]	吉沐祥, 杨敬辉, 吴祥, 等. 草莓炭疽病的生物防治[J]. 江苏农业学报, 2012, 28(6): 1498-1500.
[7]	陈官菊, 厉晓腊, 金轶伟, 等. 草莓炭疽病的发生危害和药剂防治[J]. 浙江农业科学, 2010, 51(6): 1344-1346. DOI: 10.16178/j.issn.0528-9017.2010.06.055.
[8]	邱德文. 生物农药的发展现状与趋势分析[J]. 中国生物防治学报, 2015, 31(5): 679-684. DOI: 10.16409/j.cnki.2095-039x.2015.05.008.
[9]	MUKHOPADHYAY R, KUMAR D. Trichoderma: A beneficial antifungal agent and insights into its mechanism of biocontrol potential[J]. Egyptian Journal of Biological Pest Control, 2020, 30(1): 133. DOI: 10.1186/s41938-020-00333-x.
[10]	张成, 廖文敏, 薛鸣, 等. 棘孢木霉DQ-1分生孢子固体发酵优化及其对4种作物幼苗生长的影响[J]. 中国生物防治学报, 2021, 37(2): 315-322. DOI: 10.16409/j.cnki.2095-039x.2021.02.002.
[11]	孙丽丽, 曹传旺, 薛绪亭, 等. 棘孢木霉可湿性粉剂研制及杀菌活性测定[J]. 北京林业大学学报, 2015, 37(6): 45-52. DOI: 10.13332/j.1000-1522.20140438.
[12]	邹佳迅, 范晓旭, 宋福强. 木霉(Trichoderma spp.) 对植物土传病害生防机制的研究进展[J]. 大豆科学, 2017, 36(6): 970-977. DOI: 10.11861/j.issn.1000-9841.2017.06.0970.
[13]	张广志, 杨合同, 张新建, 等. 木霉现有种类名录[J]. 菌物学报, 2014, 33(6): 1210-1230. DOI: 10.13346/j.mycosystema.140183.
[14]	KUMAR G, MAHARSHI A, PATEL J, et al. Trichoderma:A potential fungal antagonist to control plant diseases[J]. Satsa Mukhapatra-Annual Technical Issue, 2017(21):206-218.
[15]	LORITO M, WOO S L, HARMAN G E, et al. Translational research on Trichoderma: From 'omics to the field[J]. Annual Review of Phytopathology, 2010, 48: 395-417. DOI: 10.1146/annurev-phyto-073009-114314.
[16]	卢洁. 草莓种质资源炭疽病抗性分析及生防菌株筛选[D]. 沈阳: 沈阳农业大学, 2023. DOI: 10.27327/d.cnki.gshnu.2023.000499.
[17]	KUMAR S, THAKUR M, RANI A. Trichoderma:Massproduction,formulation,qualitycontrol,delivery and its scope in commercialization in India for the management of plant diseases[J]. African Journal of Agricultural Research, 2014, 9(53):3838-3852.DOI:10.5897/AJAR2014.9061.
[18]	廉华, 刘静依, 马光恕, 等. 生物炭和木霉菌对黄瓜生长及枯萎病防治效果的影响[J]. 干旱地区农业研究, 2024, 42(6): 206-215.
[19]	王丹丹, 张庆银, 李燕, 等. 枯草芽孢杆菌和哈茨木霉菌对番茄灰霉病的防治效果[J]. 河北农业科学, 2024, 28(1): 66-73. DOI: 10.12148/hbnykx.20240022.
[20]	陈捷, 朱洁伟, 张婷, 等. 木霉菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2011, 27(2): 145-151. DOI: 10.16409/j.cnki.2095-039x.2011.02.002.
[21]	罗中钦, 程琳, 张茜, 等. 丝状真菌PCR模板DNA的快速制备方法[J]. 生物技术通报, 2015, 31(9): 79-83. DOI: 10.13560/j.cnki.biotech.bull.1985.2015.09.010.
[22]	李纪顺, 陈凯, 王贻莲, 等. 防治西洋参立枯病木霉菌株的筛选鉴定及其小区防治效果[J]. 山东科学, 2019, 32(5): 62-70. DOI: 10.3976/j.issn.1002-4026.2019.05.007.
[23]	扈进冬, 隋丽娜, 李玲, 等. 深绿木霉HB20111产挥发性物质及其功能分析[J]. 植物保护, 2021, 47(5): 58-63. DOI: 10.16688/j.zwbh.2020655.
[24]	朱炳煜, 王嘉艳. 草莓炭疽病的发生、检测与防治[J]. 农业知识, 2024(9/10): 63-65.
[25]	赵玳琳, 何海永, 吴石平, 等. 棘孢木霉GYSW-6m1对草莓炭疽病的生防机制及其防病促生作用研究[J]. 中国生物防治学报, 2020, 36(4): 587-595. DOI: 10.16409/j.cnki.2095-039x.2020.04.020.
[26]	韩国兴, 礼茜, 孙飞洲, 等. 杭州地区草莓炭疽病病原鉴定及其对多菌灵和乙霉威的抗药性[J]. 浙江农业科学, 2009, 50(6): 1169-1172. DOI: 10.3969/j.issn.0528-9017.2009.06.046.
[27]	陈哲, 黄静, 赵佳, 等. 防治草莓炭疽病的芽胞杆菌组合的筛选[J]. 中国生物防治学报, 2018, 34(4): 582-588. DOI: 10.16409/j.cnki.2095-039x.2018.04.013.

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

菌株	平板对峙法菌落半径/cm	平板对峙法抑制率/%
HB 23422	1.12±0.02^b	74.55
CK	4.40±0.01^a	—

菌株	平板对扣法菌落半径/cm	平板对扣法抑制率/%
HB 23422	1.96±0.05^b	65.00
CK	5.60±0.08^a	—

处理	发病率/%	株高/cm	鲜重/g	发病指数	抑菌率/%
CK	—	13.39±0.55^c	6.09±0.37 ^ab	—	—
CM9	92.31	—	1.28±0.17 ^c	84.62	—
HB 23422-CM9	36.36	15.38±1.18 ^ab	5.29±0.46^a	41.82	60.61

Screening,identification and functional evaluation of trichoderma strains antagonistic to strawberry anthracnose

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 27

Related Articles 6

Metrics

Comments

Recommended 0

[1]	WANG Xingqiang, LI Hongmei, XU Weisheng, WEI Yanli, CHEN Kai, LI Jishun. The isolation and identification of the southern blight-causing pathogen in Houttuynia cordata Thunb. and screening of its biocontrol agent Trichoderma spp. [J]. Shandong Science, 2025, 38(4): 46-55.
[2]	JIANG Yanqing, YANG Jianwen, LI Yang, SUN Haisen, HU Jindong, LI Jishun, YANG Hetong, WU Yuanzheng. Isolation and identification of Trichoderma strains against ginger root rot [J]. Shandong Science, 2025, 38(4): 56-66.
[3]	ZHANG Jiahui, LI Hongmei, CHEN Dongmei, YANG Han, HU Jindong, LI Jishun, WEI Yanli. Identification of pathogens causing root rot of American ginseng in Weihai and screening of biocontrol Trichoderma strains [J]. Shandong Science, 2025, 38(4): 67-77.
[4]	TAI Shaohua, HU Jindong, WEI Shaowen, HONG Bo, WANG Xixin, SUN Youmin. Screening,identification, and functional evaluation of low temperature degradation of straw Trichoderma [J]. Shandong Science, 2023, 36(2): 50-58.
[5]	HU Guang-yan, ZHAO Zhong-juan, YANG He-tong. Optimization of growth and spore-producing conditions of salt-tolerant Trichoderma atroviride TW320 and Trichoderma koningiopsis TW1876 [J]. Shandong Science, 2022, 35(6): 65-73.
[6]	WANG Yi-lian, HUANG Ding-li, WEI Yan-li, LI Hong-mei, YANG Hong-tong, LI Ji-shun. Soil conditioner and Trichoderma LTR-2 combined application for remediation of continuous cropping obstacle soil in Brassica chinensis L. [J]. Shandong Science, 2022, 35(6): 74-79.