Abstract:

The chemical composition of Radix Dipsaci was separated by D101 macroporous adsorption resin, MCI column chromatography, and preparative high-performance liquid chromatography, and the structures of the isolates were identified based on NMR, HR-ESI-MS, and other spectral methods. Thirteen compounds were isolated and obtained from the 70% ethanol extract of Radix Dipsaci, including five triterpenoid saponins: asperosaponin Ⅵ (1), cauloside A (2), dipsacus saponin A (3), 3-O-(2-O-acetyl)-α-L-arabinopyranosyl-hederagenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(4),3-O-(4-O-acetyl)-α-L-arabinopyranosyl-hederagenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (5), six iridoid glycosides: loganic acid (6) loganin (7), sweroside (8), dipsanoside A (9), dipsanoside B (10), sylvestroside I (11) and two other compounds: 5-hydroxymethyl-2-furfural (12) and α-linolenic acid (13), in which compounds 4, 12, 13 were isolated from the genus of Dipsacus Linn. for the first time. The chemical composition of Radix Dipsaci was further enriched in this study that provides the material basis for establishing quality standards for Radix Dipsaci based on Q-markers as the basis for evaluating the quality of Chinese materia medica. This further provides the chemical material basis for further explaining the chemical composition and content changes before and after the processing of “sweating” and the research of the chemical composition transformation mechanism of Radix Dipsaci.

Key words: Radix Dipsaci, chemical composition, identification, triterpenoid saponins, iridoid glycosides