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    20 October 2022 Volume 35 Issue 5
      
    Traditional Chinese Medicine and Natural Active Products
    Chemical composition of Radix Dipsaci
    ZHANG Cheng-gang, YANG Chang-gui, XIAO Cheng-hong, FAN Di, GONG An-hui, HUANG Yan, ZHOU Tao
    Shandong Science. 2022, 35(5):  1-9.  doi:10.3976/j.issn.1002-4026.2022.05.001
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    The chemical composition of Radix Dipsaci was separated by D101 macroporous adsorption resin, MCI column chromatography, and preparative high-performance liquid chromatography, and the structures of the isolates were identified based on NMR, HR-ESI-MS, and other spectral methods. Thirteen compounds were isolated and obtained from the 70% ethanol extract of Radix Dipsaci, including five triterpenoid saponins: asperosaponin Ⅵ (1), cauloside A (2), dipsacus saponin A (3), 3-O-(2-O-acetyl)-α-L-arabinopyranosyl-hederagenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(4),3-O-(4-O-acetyl)-α-L-arabinopyranosyl-hederagenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (5), six iridoid glycosides: loganic acid (6) loganin (7), sweroside (8), dipsanoside A (9), dipsanoside B (10), sylvestroside I (11) and two other compounds: 5-hydroxymethyl-2-furfural (12) and α-linolenic acid (13), in which compounds 4, 12, 13 were isolated from the genus of Dipsacus Linn. for the first time. The chemical composition of Radix Dipsaci was further enriched in this study that provides the material basis for establishing quality standards for Radix Dipsaci based on Q-markers as the basis for evaluating the quality of Chinese materia medica. This further provides the chemical material basis for further explaining the chemical composition and content changes before and after the processing of “sweating” and the research of the chemical composition transformation mechanism of Radix Dipsaci.

    Identification of antioxidant components in peony flowers through on-line screening using high performance liquid chromatography-quadrupole time of flight mass spectrometer
    TIAN Xuan, LIU Yu, ZHU Wen-qing, ZHENG Zhen-Jia
    Shandong Science. 2022, 35(5):  10-15.  doi:10.3976/j.issn.1002-4026.2022.05.002
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    A high performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) combined with on-line removal of 1,1-diphenyl-2-picrylhydrazyl (DPPH) model was established for the rapid screening and identification of antioxidant active ingredients in peony flowers. Waters X-Bridge C18 chromatographic column was used with acetonitrile-water (0.1% formic acid) as the mobile phase. The peony flower samples were analyzed using electrospray positive and negative ion modes. The samples separated using HPLC were mixed with free-radical solution, and the mixture was entered into HPLC-Q-TOF-MS for rapid identification. In total, 15 ingredients and 5 antioxidant compounds were identified in the negative ion mode. This method is a simple method for rapid screening and identification of antioxidant compounds in a complex system of natural products. Furthermore, it can also provide scientific basis for further studies on peony flower.

    Pharmacology and Toxicology
    Prediction of the mechanism of Astragali Radix - Curcumae Rhizomain in the treatment of gastric cancer using network pharmacology and molecular docking
    HUANG Yue, FANG Chong-kai, NIE Duo-rui, LAN Qing-xia, LI Yue-jun, HUANG Xue-wu
    Shandong Science. 2022, 35(5):  16-25.  doi:10.3976/j.issn.1002-4026.2022.05.003
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    To explore the mechanism of Astragali Radix - Curcumae Rhizoma in the treatment of gastric cancer using network pharmacology and molecular docking. The active ingredients of Astragali Radix-Curcumae Rhizoma were searched using the traditional Chinese medicine systems pharmacology database and analysis platform. Using gastric cancer as a keyword, the associated target genes of gastrocarcinoma were retrieved from the GeneCards database. Cytoscape 3.7.0 software was used to establish an active-ingredient target network, and the STRING online database was used to construct a protein-protein interaction network using the screened targets. Gene ontology analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were conducted using the Omicshare platform. Molecular docking was performed using AutoDock Vina and PyMol software. In total, 26 active ingredients, including quercetin, kaempferol, curcumol, (3R)-3-(2-hydroxy-3,4-dimethoxyphenyl)chroman-7-ol, and beta-elemene, were screened from Astragali Radix - Curcumae Rhizoma and were identified to affect the treatment of gastric cancer via 74 targets, including TP53MYCCASP3AKT1JUN, through different signal pathways, such as cancer pathways, proteoglycans in cancer, PI3K-Akt signaling, MAPK signaling, Rap1 signaling, TNF signaling, and FoxO signaling. Using network pharmacology and molecular docking, the results of this study reflect the potential mechanism of Astragali Radix - Curcumae Rhizoma in the treatment of gastric cancer, providing evidence for its clinical application.

    Mechanism of the anti-breast cancer effect of Sparganii Rhizoma-Curcumae Rhizoma herbal pair based on network pharmacology and molecular docking
    LIU Xue-ting, SUN Xiao-hui, ZHU Jian-min, LI Lin, LI Wen-yue
    Shandong Science. 2022, 35(5):  26-36.  doi:10.3976/j.issn.1002-4026.2022.05.004
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    To explore the active ingredients and mechanisms of Sparganii Rhizoma-Curcumae Rhizoma herbal pair in breast cancer using network pharmacology and molecular docking. The effective active ingredients and corresponding targets of Sparganii Rhizoma and Curcumae Rhizoma were searched and screened using the traditional Chinese medicine systems pharmacology database and analysis platform,and the disease targets of breast cancer were searched using the OMIM and GeneCards databases. Cytoscape 3.7.2 software was used to construct a visual network diagram of drug-ingredient-disease-targets. A protein-protein interaction network (PPI) was constructed using STRING database platform, and the MCODE plug-in in Cytoscape was used for analysis to screen the top 10 core targets. Gene ontology and Kyoto encyclopedia of genes and genomes(KEGG) enrichment pathway analyses of the targets of action were performed using DAVID database, and the network diagram of ingredient-target-pathway was plotted. Finally, molecular docking was performed using autodock Vina and other software to verify the interaction between the core ingredients and the core targets. Seven active components and 73 action targets were obtained through screening. There were 43 common targets of Sparganii Rhizoma and Curcumae Rhizoma and breast cancer. The active components were hederagenin, beta-sitosterol, formononetin, stigmasterol, and trans-gondoic acid. The PPI network showed that the key targets were JUN, CASP3, PTGS2, ESR1, MPK14, PPARG, SIRT1, NOS3, TGFB1 and NOS2. The KEGG pathway enrichment analysis revealed 72 items. According to the P values, the first 20 items related to breast cancer were eliminated via screened, including the PI3K-Akt signaling pathway, VEGF signaling pathway, p53 signaling pathway, and MAPK signaling pathway. The molecular docking results showed that the active ingredients had strong binding ability in docking with the core targets. Therefore, our results suggest that Sparganii Rhizoma-Curcumae Rhizoma herbal pair has characteristics of a multi-ingredient, multi-target, and multi-pathway on breast cancer, which provides a theoretical basis for its clinical application in the future.

    Exploring the medication rules and mechanisms of traditional Chinese medicine involved in the treatment of peptic ulcer based on data mining and network pharmacology
    LIU Yuan, SUN Hui, XU Yuan-nan, YANG Min, MA Zhi-qiang, LI Chi-chuan, ZHAO Shuang-li, ZHAO Tai-ying, CHEN Jie
    Shandong Science. 2022, 35(5):  37-45.  doi:10.3976/j.issn.1002-4026.2022.05.005
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    Data mining and network pharmacology were used to investigate the rules of using traditional Chinese medicine for treating peptic ulcer and to screen the potential targets of core drugs to study their mechanism of action in the treatment of peptic ulcer. The core pairs were derived via frequency and association rule analyses, and the active components and targets of the core pairs were screened. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses of the key targets of core drugs for the treatment of peptic ulcer were performed. A component-target-signaling pathway network was established. Results revealed that the 233 prescriptions for the treatment of peptic ulcer included 216 traditional Chinese medicines, of which Poria- Atractylodis Macrocephalae Rhizoma was the core pair. The 22 chemical components of the core drug pair corresponded to 143 potential targets, among which 41 intersected with peptic ulcer. Through GO analysis, 92 biological processes, 5 molecular functions, and 11 cellular compositions were identified, and through and KEGG analysis, 15 signaling pathways. The core drugs can exert the therapeutic effect on peptic ulcer through JUN,IL6,TP53,AKT1,VEGFA,MAPK1,CASP3,EGFR,RB1,and IL1B target, regulating IL-17, PI3K-AKT, NF-κB, and epithelial cell signaling in Helicobacter pylori infection and other related pathways. In this study, we explored the rules of Traditional Chinese Medicine for peptic ulcer and preliminarily elaborated the pharmacological effects of the core drug pair Poria- Atractylodis Macrocephalae Rhizoma for the treatment of peptic ulcer to provide references for clinical dosing and new drug research.

    New Materials
    Investigation of cold-welding remanufacturing process and performance of camshaft working surface's pitting corrosion
    SUN Xiu-huai, YIN Zi-qiang, WANG Shou-ren, WANG Lei, WU Cheng-wu, ZHANG Jian-peng, LI Chong-yang
    Shandong Science. 2022, 35(5):  46-52.  doi:10.3976/j.issn.1002-4026.2022.05.006
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    The working surface of a camshaft is easily worn during service, thus seriously affecting the normal operation of the machine. The pitting areas on the working surface of the camshaft were repaired using cold-welding equipment. A metallographic microscope was used to observe the microstructure of the repaired area, and the residual stress distribution and hardness distribution around the repaired areas were determined using an X-ray residual stress tester and a hardness tester, respectively. The results show that the repair welding spot is well combined with the matrix, the carbides precipitated from austenite are dispersed in the matrix structure, the position of the repair welding spot presents the minimum residual stress and the minimum hardness value, and the influence of the repair welding spot on the residual stress and hardness is maintained within around 4 mm of the repaired areas. The camshaft repaired via cold welding meets the service performance requirements.

    Synthesis and property of polyimide films coupling with biomass fibers
    ZHAO Xin-fu, CAO Ge-ge, YI Xi-bin, ZHAO Guan-wei, NIE Yi-hao, GUO Yan-jun, FANG Chang-long
    Shandong Science. 2022, 35(5):  53-60.  doi:10.3976/j.issn.1002-4026.2022.05.007
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    Polyimide (PI), a type of high temperature-resistant polymer synthesized using dianhydride or diamine, has become one of the most industrialized polymer materials with the highest operating temperature owing to its strong high-temperature resistance property. However, the strength of PI films still needs to be improved for their industrial application. To improve the elongation rate and tensile strength of PI films, different methods, such as adjusting the concentration, ratio, addition method of precursors and the addition amount of biomass fibers, have been tested in our experiments.4,4-Diaminodiphenyl ether and 3,3,4,4-biphenyltetracarboxylic acid dianhydride were used as the precursors for the reaction. Polyamide acid was prepared using the positive addition method in the presence of a cross linker, which was then poured into a Petri dish containing uniform lotus root fiber. The PI film was obtained by calcination. The results showed that the final gage length and elastic modulus of the PI films containing lotus root fiber were lower than those of the pure PI film. Furthermore, PI-2% root-fiber film had a tensile elongation at break of 6.49%, a tensile strength of 67.33 MPa, and a maximum force tolerance.of 57.47 N, which are much higher than those of pure PI film. Thus, the addition of lotus root fiber enhances the mechanical properties of the film.

    Energy and Power
    Computational fluid dynamic simulation on valve leakage in power plants
    ZHANG Bing, ZHANG Li, CHEN Zhi-qiang, LIU Guang-di, ZHAO Hong-xia
    Shandong Science. 2022, 35(5):  61-68.  doi:10.3976/j.issn.1002-4026.2022.05.008
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    Currently, the leakage of drain pipeline values in power plants is detected automatically using the principle of heat transfer. However, existing studies have not yet analyzed the flow and heat transfer of the fluid in a pipeline during valve leakage. Furthermore, research on the arrangement of temperature measurement points and the accuracy requirements of temperature measurements is lacking. To address these shortcomings, this study uses a computational fluid dynamic simulation to investigate heat transfer and flow in pipelines when valve leakage occurs. In addition, the influence of different pipeline diameters and insulation materials on differences in the measured temperatures and the amount of leakage is analyzed. The findings of this study provide a reference for the real-time monitoring of dynamic changes in the flow near the valve and the diagnosis of leakage faults of drain valves on engineering sites.

    Prediction of hydrate formation of natural gas containing moisture and sulfur in ejector
    XU Tao, LIU Wei, WANG Zhao-liang
    Shandong Science. 2022, 35(5):  69-79.  doi:10.3976/j.issn.1002-4026.2022.05.009
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    For the continuous decrease in the wellhead pressure of natural gas containing moisture and sulfur in gas fields with high quantities of sulfur, low-pressure gases are transported owing to the surplus pressure from high-pressure gas wells via ejectors. Herein, the Fluent software is used to numerically simulate temperature and pressure profiles for single- and two-phase flows of natural gas containing moisture and sulfur in an ejector. The ZahediⅠmodel is used to predict the formation area of natural gas hydrates in the ejector. The effect of the inlet temperature of the working fluid, sulfur content, and moisture content on the formation of natural gas hydrates is predicted and analyzed. With increasing inlet temperature of the working fluid, the generation area range of natural gas hydrates in the ejector decreases. When the sulfide content is high, the generation area range of natural gas hydrates is large. The working fluid contains water droplets. The generation area of natural gas hydrates in the ejector is smaller than that under a single-phase working medium. Based on these results, measures for reducing natural gas hydrates are proposed.

    Traffic and Transportation
    Severity analysis of highway production loss based on partial odds ratio
    YAO Hong-yun, CAO Zhi-fu, TU Qiang
    Shandong Science. 2022, 35(5):  80-88.  doi:10.3976/j.issn.1002-4026.2022.05.010
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    Based on the data of 814 accidents that caused road property losses on the southwest mountain expressway, to clarify the correlation factors of the road-property-loss severity, the road property losses caused by accidents are divided into three levels according to the amount of compensation: <4 000, 4 000~10 000, and >10 000 RMB. Further, potential factors affecting the severity of road property losses are selected from the aspects of vehicle type, number of vehicles involved, accident form and cause, and driving environment. An analysis model of the road-property-loss severity on mountain highways is established using partial advantage ratio model, and the degree of influence on road property loss due to various factors is analyzed via a pseudoelastic coefficient. The results show that 13 factors are considerably related to the degree of road property loss. Among these factors, the proportional advantage assumption is not met when the vehicles involved in accidents are small and medium-sized trucks, buses, and cars and the season is in winter, and the remaining factors meet the proportional advantage assumption. When the vehicle involved in an accident is a heavy truck, it increases the probability of a Level 2 and Level 3 road-property-loss accident; the same happens at the sections of the road with a small curve radius. In addition, smooth curve section, multivehicle accidents, weather, and straight sections will cause an increase in the probability of Level 2 and Level 3 road-property-loss accidents.

    Location planning of a cold chain logistic center for warehousing in rural areas
    XIE Long, ZHANG Xiao-dong
    Shandong Science. 2022, 35(5):  89-96.  doi:10.3976/j.issn.1002-4026.2022.05.011
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    This study addresses the problems associated with the storage and loss of “the first kilometer” of agricultural produce logistics in rural areas. As an example, areas with cold storage requirements are considered and the location model of a cold chain logistic center is proposed under the operation guideline of local refrigeration, direct delivery from the origin for cold chain produce logistics in rural areas. Because fresh agricultural products are perishable, requirements for transportation and refrigeration are high. Therefore, the location model introduces time constraints. The objective is to minimize the total cost of transportation, investment, construction and operation. The model is solved using the Gurobi optimizer, achieving the optimal solution that meets time-related requirements and ensuring the rationality of the warehouse location planning results. Finally, ten alternative addresses are determines. Results show that the cost of building seven cold chain storage centers is the lowest. The findings of this study confirm the feasibility of the location model of the cold chain logistic center in rural areas by considering product types, costs, and time.

    Research and development on rulers driver automation for railway signaling interlocking system
    HU Tian
    Shandong Science. 2022, 35(5):  97-103.  doi:10.3976/j.issn.1002-4026.2022.05.012
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    Computer interlocking system is a key subsystem of railway transportation system that requires high safety and reliability. For a long time, fully covering the test of railway signal interlocking system was difficult because of the large amount of data. This paper focuses the research and development on a rule-driven automated platform that integrates expert experience into automatic testing and can generate test scripts based on rules to cover all signal devices. It canalso protract track map and perform scripts in a single batch. Test results against an interlocking system of a special line show that the platform is especially suitable for medium- and large-scale station testing, and can be easily extended to the testing of other security systems.

    Joint site selection of multi retail formats in new type of retail network
    TANG Yun-feng, ZHANG Ze-jian, LI Rong-sen, ZHANG Ke, LUO Qian-wen, CHENG Yu
    Shandong Science. 2022, 35(5):  104-111.  doi:10.3976/j.issn.1002-4026.2022.05.013
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    In this study, the site selection of multi retail formats of supermarkets in the new retail network is investigated. By combining the geographic information system and fuzzy analytic hierarchy process, first, some alternative construction sites are selected from several available construction sites of supermarkets in a region. Then, the requirements of the number, business type, and coverage of supermarkets are considered. A mathematical model is established with the minimum total cost as the objective function, and the optimal site result is achieved using Matlab. Finally, the comprehensive site selection method is employed using a practical example.

    Environment and Ecology
    Research progress of material stock based on bibliometric analysis
    YANG Dong, DANG Meng-yuan, HAN Feng, SHI Feng
    Shandong Science. 2022, 35(5):  112-121.  doi:10.3976/j.issn.1002-4026.2022.05.014
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    Based on the bibliometric analysis method, with the help of CiteSpace and VOSviewer visualization analysis software, this study, by combining quantitative and qualitative methods, systematically classifies the results of material stock research performed at home and abroad and analyzes the knowledge base, development context, and research progress of material stocks. Metals and buildings are found to be essential in material stock research and lay the basic foundation for this research. The research topics of material stocks include simple materials accounting to resource prediction, socioeconomic metabolism analysis, greenhouse gas emission reduction, and urban mineral development. The research methods are constantly enhanced, and life cycle assessment, decoupling analysis, and scenario analysis are introduced to expand the scope of this research. Furthermore, the research content of material stocks is enhanced, which involves greenhouse gas emission, circular economy, urban mineral resources, resource efficiency, and other sustainable development content. The existing problems in the development of material stock research are discussed, such as the difficulty in obtaining key data, data with different qualities, and practical guidance still need to be strengthened. The future development of material stock research should be actively integrated with other disciplines to improve the data accuracy and strengthen the ability of decision support services..

    Distribution pattern of natural ecosystems in the Yellow River region in Henan based on national geoinformation data
    HU Chan-juan, LI Shuang-quan, GUO Lei, DU Jun, LIU Peng, REN Jie
    Shandong Science. 2022, 35(5):  122-129.  doi:10.3976/j.issn.1002-4026.2022.05.015
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    Based on the monitoring data of national geoinformation, this study analyzes the distribution pattern and change characteristics of natural ecosystems, including forest land, grassland, wetland, and bare land, from 2015 to 2019 in eight cities and one county along the Yellow River in Henan Province, China. Results show that the coverage rates of forest land, grassland, wetland, and bare land along the Yellow River are 36.12%, 2.61%, 2.01%, and 0.56%, respectively. Forest land was dominated by broad-leaved forests and broad-leaved shrub forests. The land coverage of coniferous forests, arbor-shrub-mixed forests, afforested land, and artificial young forests increased, and the land coverage of broad-leaved, coniferous, and broad-leaved shrub-mixed forests decreased during the study period. Grassland was dominated by high-coverage grassland, accounting for 81.47% of the grassland area. During the study period, the area of high-coverage grassland, artificial green grassland, and slope-protected shrub grassland increased and the area of medium-coverage grassland and herbage grassland decreased. The wetland was dominated by rivers, occupying 97.63% of the total wetland area. During the study period, the area of rivers and canals increased. The gravel surface was dominant, accounting for >60% of the total bare land. During the study period, the coverage area of all types of bare land showed a downtrend. Overall, in the Yellow River region of Henan Province, the distribution characteristics of different natural ecosystems differed; forest land was mainly distributed in the middle reaches, while the grassland was mainly distributed in the low reaches; the spatial distribution of the wetland area in the low reaches was higher than that in the middle reaches,while the area of bare land in the middle reaches was higher than that in the low reaches. During the study period, the coverage of forest land and bare land decreased, while that of grassland and wetland increased.

    Other Research Articles
    Design and implementation of autonomous navigation system comprising a hydraulic driven robot based on robot operating system
    LIU Guang-liang, MA Zheng-guang, ZHU Lin, XIAO Yong-fei, ZHANG Yan-fang
    Shandong Science. 2022, 35(5):  130-139.  doi:10.3976/j.issn.1002-4026.2022.05.016
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    In this study, a robot track equation is formulated to estimate the state and position of the robot based on a two-wheel differential drive model. Using the robot operating system(ROS) stack package, we design a robot structural model with seven joints and links, including a hydraulic robot body, a robot base, hydraulic motors, guide wheels, and lidar. The transformation of coordinate frames is shown over the Tf of ROS to obtain the coordinates of the robot's position. Because our robot does not possess an encoder and vision system, we use a two-dimensional (2D) planar lidar odometric model (RF2O). The planar motion estimation of the robot is performed by imposing the range flow constraint equation on consecutive lidar scan pairs. Then, the velocity of the lidar sensor and the real-time trajectory of the robot can be estimated. We also implement an autonomous navigation software system with functions such as navigation map building, navigation targeting, and multirobot management. We evaluate the accuracy of the system several times. Based on a comparative analysis of the difference in the data of assigned and navigated locations, the accuracy of robot navigation and position is found to satisfy the design requirements.

    Analysis of the holiday climate index variation characteristics in Henan Province for the past 30 years
    ZHANG Wei, LIU Yu-zhu
    Shandong Science. 2022, 35(5):  140-146.  doi:10.3976/j.issn.1002-4026.2022.05.017
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    In this study, the spatial and temporal variation characteristics of holiday climate in Henan province are analyzed using the ground meteorological data (including daily maximum temperature, average relative humidity, precipitation, wind speed, and cloud-coverage rate) from 111 national meteorological observatories in Henan province for the past 30 years (1991—2020). The results show that the holiday climate index(HCI) of Henan reached a “suitable” or a more than “suitable” level from January to December, and the monthly variations present bimodal characteristics. In April to May and September to October, reaching the highest level “particularly suitable”. The Henan HCI indicates that the seasonal variations are the highest in spring and autumn, followed by summer, and the lowest in winter, the spatial distribution presents the “high in north and low in south” characteristics, and the seasonal distribution presents certain dimensional characteristics and obvious regional characteristics.