SHANDONG SCIENCE ›› 2018, Vol. 31 ›› Issue (4): 85-88.doi: 10.3976/j.issn.1002-4026.2018.04.014
• Biosensors • Previous Articles Next Articles
ZHAO Kai, GE Jing-hua, WANG Hai
Received:
Online:
Published:
Abstract:
In order to increase the expression of phytase in Pichia pastoris, the fermentation conditions of phytaseproducing recombinant Pichia pastoris in 50 L fermentation tank were optimized. The results show that the strain wet weight at 300 g/L by feeding glucose, before addition of methanol, got the phytase activity 21 666 U/mL, 12.9% higher than the control by the end of fermentation, which is most favorable for the production of phytase by fermentation.
Key words: Pichia pastoris, phytase, the wet cell weight before induction, fermentation optimization
CLC Number:
ZHAO Kai, GE Jing-hua, WANG Hai. Influence of the wet cell weight before induction on secretive expression of phytase in Pichia pastoris[J].SHANDONG SCIENCE, 2018, 31(4): 85-88.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: https://www.sdkx.net/EN/10.3976/j.issn.1002-4026.2018.04.014
https://www.sdkx.net/EN/Y2018/V31/I4/85
[1]王红宁,黄勇,陈惠. 饲用微生物植酸酶的研究进展[J]. 饲料广角,1998(5):1720. [2]王红宁,吴琦,谢晶,等. 真菌植酸酶phyA基因研究进展[J]. 四川农业大学学报,2000,18 (1) :8488. [3]蒋守群,吴天星. 植酸酶的研究进展[J]. 动物营养学报,1999,11 (3) :111. [4]CANTOR A H, PERNEY K M. 家禽日粮中添加植酸酶可提高磷利用率和降低动物粪便磷环境污染[J]. 中国饲料,1993 (1):4043. [5]周晨研,刘振华,王丹丹,等. 产木聚糖酶毕赤酵母工程菌株构建及表达条件优化研究[J]. 食品工业科技,2016(13):162167. [6]王停停,郑宏臣,徐健勇,等. 木聚糖酶XynG11在毕赤酵母中的表达及发酵条件优化[J]. 食品与发酵工业,2017,43(1):3743. [7]孙玮遥,宋增健,王向东,等. 重组毕赤酵母表达蛋清溶菌酶发酵条件的优化[J]. 中国酿造,2017,35(2):5457. [8]宋玛丽,王茜,杜军,等. 重组植酸酶appA2QN 在毕赤酵母中的高效表达[J]. 华北农学报,2015,30 (2):5558. [9]黄魁英,夏枫耿,黄乐天,等. 耐高温植酸酶毕赤酵母工程菌发酵中试条件优化[J]. 现代食品科技,2011,27 (10):12381241. [10]MEENAKSHISUNDARAM S. Gene cloning, expression and optimization of phytase production in pichia pastoris[J]. Research Journal of Biotechnology,2014,9 (12):4153. [11]武婕,张晓雪,余河水,等. 毕赤酵母工程菌高密度发酵研究与进展[J]. 中国生物工程杂志,2016,36(1):108114. [12]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB/T 18634—2009饲用植酸酶活性的测定 分光光度法[S].北京:中国标准出版社,2009.
Cited