SHANDONG SCIENCE ›› 2018, Vol. 31 ›› Issue (4): 79-84.doi: 10.3976/j.issn.1002-4026.2018.04.013

• Biosensors • Previous Articles     Next Articles

A study of optimal fermentation conditions for phytase production by engineered strain

YANG Jun-hui, GONG Wei-li, YANG Yan, MENG Qing-jun, SHI Jian-guo*   

  1. Shandong Provincial Key Laboratory for Biosensors, Biology Institute, Qilu University of Technology  (Shandong Academy of Sciences), Jinan 250014,China
  • Received:2018-03-16 Online:2018-08-20 Published:2018-08-20

Abstract:

To study the factors affecting phytase production by engineered Pichia pastoris, we explored the optimal fermentation conditions, such as methanolfedbatch concentration or induction time, with shake flask fermentation. The results demonstrated that a series of factors including residual glycerol, methanol concentration and induction time had significant effects on phytase production. Residual glycerol could delay the phytase secretion, 1.5% methanol had the best effect of induction with enzyme activity reaching about 1 100 U/mL in the second day, while low concentration of methanol could only slowly induce enzyme accumulation, and high concentration of methanol was harmful to engineered strain. This study lays a foundation for further optimization of high density fermentation conditions.

Key words: Pichia pastoris, optimal fermentation conditions, phytase, methanol

CLC Number: 

  • Q936