植酸酶工程菌产酶条件优化研究

doi:10.3976/j.issn.1002-4026.2018.04.013

Abstract

Abstract:

To study the factors affecting phytase production by engineered Pichia pastoris, we explored the optimal fermentation conditions, such as methanolfedbatch concentration or induction time, with shake flask fermentation. The results demonstrated that a series of factors including residual glycerol, methanol concentration and induction time had significant effects on phytase production. Residual glycerol could delay the phytase secretion, 1.5% methanol had the best effect of induction with enzyme activity reaching about 1 100 U/mL in the second day, while low concentration of methanol could only slowly induce enzyme accumulation, and high concentration of methanol was harmful to engineered strain. This study lays a foundation for further optimization of high density fermentation conditions.

Key words: Pichia pastoris, optimal fermentation conditions, phytase, methanol

CLC Number:

Q936

YANG Jun-hui, GONG Wei-li, YANG Yan, MENG Qing-jun, SHI Jian-guo. A study of optimal fermentation conditions for phytase production by engineered strain[J].SHANDONG SCIENCE, 2018, 31(4): 79-84.

References

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A study of optimal fermentation conditions for phytase production by engineered strain

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