SHANDONG SCIENCE ›› 2018, Vol. 31 ›› Issue (4): 85-88.doi: 10.3976/j.issn.1002-4026.2018.04.014

• Biosensors • Previous Articles     Next Articles

Influence of the wet cell weight before induction on secretive expression of phytase in Pichia pastoris

ZHAO Kai, GE Jing-hua, WANG Hai   

  1. Qingdao Vland Biotech Inc, Qingdao 266101, China
  • Received:2018-05-30 Published:2018-08-20 Online:2018-08-20

Abstract:

In order to increase the expression of phytase in Pichia pastoris, the fermentation conditions of phytaseproducing recombinant Pichia pastoris in 50 L fermentation tank were optimized. The results show that the strain wet weight at 300 g/L by feeding glucose, before addition of methanol, got the phytase activity 21 666 U/mL, 12.9% higher than the control by the end of fermentation, which is most favorable for the production of phytase by fermentation.

Key words: Pichia pastoris, phytase, the wet cell weight before induction, fermentation optimization

CLC Number: 

  • Q936

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