We address the content change of the active substances in their different growth stage with three improved varieties of Lonicera japonica Thunb. of the stages of Sanqing, Erbai, Dabai, Yinhua and Jinhua as objects. Experimental results show that the yield of Dabai stage is the highest, whose value is 9.68 g, 6.69 g and 9.86 g. Drying rate of Sanqing stage is the highest while the content of soluble sugar is the highest in Dabai stage. The contents of chlorogenic acid and luteoloside acid are the highest in Sanqing stage and then gradually decrease with the growth of flower. The contents of chlorogenic acid and luteoloside acid of Lonicera japonica (Yatehong LSB) are the lowest, 4.22% and 0.16%. The content of luteoloside of Yinhua stage gradully increases with the growth of flower while the content of the chlorogenic acid is the highest in Yinhua stage. Difference is not significant for the two active substances in Lonicera japonica (Yate LSB) and Lonicera japonica (Yateliben LSB). The content change of volatile oil is contrary to that of chlorogenic acid and luteoloside acid. The content of volatile oil, 1.40%, is the highest for Lonicera japonica (Yatehong LSB), and no significant distinction exists in the other two improved varieties. We discover that the best harvest stage is Erbai stage.