To eliminate the influence of electrochemically active compounds on ethanol determination in fruit juices via biosensor, an ethanol-assisted enzyme electrode was prepared and a novel alcohol biological detection system was constructed using the synergy of working and auxiliary enzyme electrodes. The anti-interference property of the system was tested and the anti-interference coefficient was fitted. Thereafter, the calculation formula of ethanol concentration was deduced to achieve the rapid and accurate detection of ethanol concentration. The results showed that the anti-interference performance of the detection was stable with good stability, accurate results, and strong ability. When the method was applied to determine the ethanol concentration in fruit juices, the relativity standard division was 0.80% and the recoveries were 99.10% to 101.10%. Glucose, sucrose, citric acid, Ca²⁺, catalase, and vatamine C in the fruit juices had no effect on ethanol detection. T-test analysis showed that there was no significant difference between this method and the national standard and gas chromatography (P>0.05).The method is simple in operation,low in cost and high in accuracy,which can meet the production requirements of low cost.

Enzyme inhibitor interference problems are often encountered when amperometric enzyme electrode biosensors are used in industrial fields. Test results of enzyme electrode biosensing detection tend to be significantly lower if enzyme activity inhibitors are present in samples. According to the detection principle of amperometric enzyme electrode biosensors, a testing method was designed to counter the interference of inhibitors. After the calibration of the biosensor, a single sample was first tested and then the same sample was added with a known standard sample and tested for the second time. The two tests were performed under the same conditions of enzyme inhibitors. Through a comparison of the results of two tests, the inhibition coefficient was calculated according to the determined response of the added standard sample at the second test.Finally, the unknown sample was calculated based on the result of the first test and inhibition coefficient. This analysis method can effectively eliminate the influence of enzyme inhibitors on the analysis and expand the application range of biosensors in industrial fields.