Abstract: Enzyme inhibitor interference problems are often encountered when amperometric enzyme electrode biosensors are used in industrial fields. Test results of enzyme electrode biosensing detection tend to be significantly lower if enzyme activity inhibitors are present in samples. According to the detection principle of amperometric enzyme electrode biosensors, a testing method was designed to counter the interference of inhibitors. After the calibration of the biosensor, a single sample was first tested and then the same sample was added with a known standard sample and tested for the second time. The two tests were performed under the same conditions of enzyme inhibitors. Through a comparison of the results of two tests, the inhibition coefficient was calculated according to the determined response of the added standard sample at the second test.Finally, the unknown sample was calculated based on the result of the first test and inhibition coefficient. This analysis method can effectively eliminate the influence of enzyme inhibitors on the analysis and expand the application range of biosensors in industrial fields.

Key words: enzyme electrode, biosensor, enzyme inhibitor, interference suppression, determination method