血样预处理与酶电极法分析

doi:10.3976/j.issn.1002-4026.2012.03.016

J4 ›› 2012, Vol. 25 ›› Issue (3): 75-79.doi: 10.3976/j.issn.1002-4026.2012.03.016

• Content • Previous Articles Next Articles

Pretreatment of blood specimens and their enzyme electrode method analysis

SHI Jian-Guo¹, LIU Jin-Yu², SUN Shi-Qing¹, ZHANG Juan³

1.Shandong Provincial Key Laboratory of Biosensors, Institute of Biology, Shandong Academy of Sciences, Jinan 250014, China; 2.Department of Pharmacy and Instrument, 456 Hospital of PLA, Jinan 250031, China; 3.Care in Department of Neurology, Shandong Provincial Hospital, Jinan 250021, China

Received:2012-02-29 Online:2012-06-20 Published:2012-06-20

Abstract

Abstract:

We employed enzyme electrode to assess the effect of glyceraldehydes and sodium fluoride on the preservation of glucose and lactic acid in blood specimens. Experimental results show that glyceraldehydes is better than sodium fluoride in the preservation of glucose contents. However, it can not serve as the inhibition regent of lactic acid. The lactic acid remains almost constant as treated with sodium fluoride at 4 ℃.

Key words: glucose, lactic acid, glyceraldehydes, sodium fluoyide, enzyme electrode

CLC Number:

R446.11

SHI Jian-Guo, LIU Jin-Yu, SUN Shi-Qing, ZHANG Juan. Pretreatment of blood specimens and their enzyme electrode method analysis[J].J4, 2012, 25(3): 75-79.

[1]	WANG Bing-lian, MA Yao-hong, GONG Wei-li, YANG Yan, ZHENG Lan, LIU Qing-ai, MENG Qing-jun. Anti-interference detection method of ethanol concentrationin fruit juices [J]. Shandong Science, 2022, 35(4): 84-89.
[2]	ZHU Si-rong, BI Chun-yuan, DU Yi, ZHANG Jin-ling, GAO Guang-heng, ZHANG Li-qun, ZHAO Xiao-hua, YANG Yan. An analysis method for suppressing interference from the reversible inhibitors of amperometric enzyme electrode biosensors [J]. Shandong Science, 2021, 34(4): 87-94.
[3]	SUN Hui-nan, GAO Guang-heng , LIU Qing-ai , MA Yao-hong, LI Jing , SHI Jian-guo. Application of blood lactic acid biosensor in the diagnosis and treatment of COVID-19 [J]. Shandong Science, 2020, 33(3): 1-6.
[4]	DU Yi, ZHANG Jin-ling, ZHU Si-rong, ZHANG Li-qun, ZHAO Xiao-hua, SHI Jian-guo, BI Chun-yuan. Determination of milk lactose content using composite enzyme membrane biosensor based on difference method [J]. Shandong Science, 2019, 32(6): 43-48.
[5]	YANG Jun-hui, MA Heng, MA Yao-hong, ZHAO Yu-bin, SUN Hui-nan, SHI Jian-guo. Rapid analysis of key biochemical parameters during starch sugar production [J]. Shandong Science, 2019, 32(5): 104-109.
[6]	YANG Jun-hui1, MA Heng, MAN De-en, GUO Mai-hai, LIU Qing-ai, MA Yao-hong, SHI Jian-guo. Study on the fermentation parameters of lactic acid bacteria [J]. SHANDONG SCIENCE, 2018, 31(5): 38-42.
[7]	ZHANG Jin-ling, DU Yi, GAO Guang-heng, ZHAO Xiao-hua, ZHANG Li-qun, ZHU Si-rong,SHI Jian-guo,BI Chun-yuan. Determination of aflatoxin B1 content in corn by enzyme electrode method [J]. SHANDONG SCIENCE, 2018, 31(1): 60-.
[8]	ZHU Sirong, ZHOU Wanli, BI Chunyuan, ZHANG Liqun, YANG Yan, ZHANG Jinling,ZHAO Xiaohua. Development of biosensors based online analysis system for fermentation process [J]. SHANDONG SCIENCE, 2016, 29(5): 90-97.
[9]	WANG Peipei， LIU Qingai，YANG Junhui，MENG Qingjun，YANG Yan，MA Yaohong. Application of lactic acid bacteria fermentation in quality improvement of buckwheat flour [J]. SHANDONG SCIENCE, 2016, 29(2): 64-67.
[10]	LIYan1,WANGPing2,DING Xiaoyan2,GAO Yan2,WEI Xia3,ZHAO Bonian2. Differences of protective effect of liposoluble components in Salvia miltiorrhiza Bge. from different origin places to H9C2 cardiac myocytes injured by oxygen/glucose deprivation [J]. SHANDONG SCIENCE, 2015, 28(2): 18-25.
[11]	BI Chunyuan,ZHANG Jinling, ZHANG Liqun,DU Yi,SHI Jianguo. Response characteristics of pyranose oxidase electrode to glucose and xylose [J]. SHANDONG SCIENCE, 2015, 28(2): 47-52.
[12]	WANG Chunlei,LIN Meng. Microporous Prussian Blue/gold composite material based enzyme electrochemical sensor [J]. SHANDONG SCIENCE, 2015, 28(2): 53-57.
[13]	SHI Jianguo,LI Yiwei,ZHANG Xianen. Research advances and development of biosensors in China [J]. SHANDONG SCIENCE, 2015, 28(1): 28-35.
[14]	MA Yao-Hong, MENG Qing-Jun, YANG Yan, YANG Jun-Hui, SHI Jian-Guo, ZHANG Li-Qun, ZHENG Lan, LIU Wei-Xia. A hydrolysis process based rapid identification approach for polysaccharides [J]. SHANDONG SCIENCE, 2014, 27(4): 12-16.
[15]	FENG Wen-Hong, MENG Qing-Jun, ZHANG Li-Qun, YANG Yan, SHI Jian-Guo. Rapid determination of the composition of corn steep water [J]. J4, 2011, 24(4): 45-49.

Pretreatment of blood specimens and their enzyme electrode method analysis

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0