基于网络药理学探讨葶苈大枣泻肺汤治疗新冠肺炎的作用机制

doi:10.3976/j.issn.1002-4026.2020.05.001

Abstract

Abstract: To analyze the mechanism of Tingli Dazao Xiefei decoction in the treatment of COVID-19,TCMSP database was used to screen the main active compounds and related targets,GeneCards database was employed to obtain the targets related with COVID-19,STRING and Cytoscape were used to get the PPI network analysis,Metascape database was applied to predict GO enrichment and KEGG pathway annotation, and Autodock was adopted for molecular docking. According to the results, there were 27 active components involved in Tingli Dazao Xiefei decoction and 39 targets aimed to COVID-19, including quercetin, kaempferol, etc., core targets such as TNF,MAPK1/8,CASP3. There were 146 KEGG pathways, from which IL-17, NF-κB, tuberculosis got the higher P value. The results of molecular docking showed that the core compounds quercetin, kaempferol, isorhamnetin and β-sitosterol of Tingli Dazao Xiefei decoction had a good affinity with ACE2. This study showed that quercetin, kaempferol, isorhamnetin of Tingli Dazao Xiefei decoction combined with ACE2, may participate in the IL-17 and NF-κB signaling pathway by regulating TNF, CASP3, MAPK, so as to inhibit cytokine storm and inflammatory injury, which could be applied to prevent COVID-19 by multi-component, multi-target and multi-pathway.

Key words: Tingli Dazao Xiefei decoction, COVID-19, network pharmacology, molecular docking

CLC Number:

R285

LIU Jing-hua, TAN Wang-xiao, WANG Si-wei, WANG Yu, WANG Xiao-ying. Study on the mechanism of Tingli Dazao Xiefei decoction in the treatment of COVID-19 based on the network pharmacology[J].Shandong Science, 2020, 33(5): 1-13.

References 49

1	袁宏丽, 范恒全, 马彦华. 新型冠状病毒肺炎与其他常见病毒性肺炎的CT鉴别诊断[J]. 解放军医药杂志, 2020, 32(4): 5-8. doi: 10.3969/j.issn.2095-140X.2020.04.002
2	陈莉莉, 葛广波, 荣艳，等. 中药在新冠肺炎防治中的应用和研究进展[J]. 上海中医药大学学报,2020,34(3):1-8. CHEN L L,GE G B,RONG Y,et al.Application and research progress of traditional Chinese medicine in prevention and treatment of coronavirus disease 2019[J]. Academic Journal of Shanghai University of Traditional Chinese Medicin,2020,34(3):1-8. doi: 10.16306/j.1008-861x.2020.03.001
3	孟宪泽, 万旭英, 李军昌, 等. 新型冠状病毒肺炎患者中医辨证规律研究[J]. 第二军医大学学报, 2020,41 (5):493-497. doi: 10.16781/j.0258-879x.2020.05.0493
4	高远, 林平, 邵岩峰, 等. 探析《新型冠状病毒肺炎诊疗方案(试行第七版)》中新冠肺炎中医分期病机特点[J]. 福建中医药, 2020, 51(2): 10-11. doi: 10.13260/j.cnki.jfjtcm.011986
5	马梅芳, 吕文海. 葶苈子近30年研究进展[J]. 中医药信息, 2005, 22(5): 35-36. doi: 10.3969/j.issn.1002-2406.2005.05.015
6	李靖珺. 浅谈葶苈子的药理作用[J]. 大家健康, 2014, 8(18): 30.
7	吴镇印, 谢红. 葶苈子治疗肺心病的理论依据[J]. 浙江中西医结合杂志, 2014, 24(6): 562-563. doi: 10.3969/j.issn.1005-4561.2014.06.045
8	曹雅雯, 汤岐梅, 侯雅竹, 等. 葶苈子治疗心力衰竭的药理研究进展[J]. 中西医结合心脑血管病杂志, 2019, 17(20): 3123-3126. doi: 10.12102/j.issn.1672-1349.2019.20.012
9	曹新福, 刘子豪, 李享, 等. 全国各地区防治新型冠状病毒肺炎中医药方案用药规律分析[J]. 北京中医药, 2020,39(5):418-422. doi: 10. 16025 /j. 1674-1307. 2020. 05. 006
10	扎亚东, 萧抒蓉, 张冲. 葶苈大枣泻肺汤加味治疗支气管哮喘的疗效分析[J]. 中国处方药, 2019, 17(11): 139-140. doi: 10.3969/j.issn.1671-945X.2019.11.085
11	牛占清. 葶苈大枣泻肺汤、导痰汤、化疗联合治疗痰瘀阻肺型肺癌的疗效观察[J]. 临床医药文献电子杂志, 2019, 6(93): 173. doi: 10.16281/j.cnki.jocml.2019.93.147
12	姚智. 葶苈大枣泻肺汤、三子养亲汤用于肺心病患者中的效果[J]. 中国社区医师, 2019, 35(16): 81, 84. doi: 10.3969/j.issn.1007-614x.2019.16.058
13	石德祥, 史金峦. 葶苈大枣泻肺汤联合西药治疗结核性胸膜炎临床研究[J]. 陕西中医, 2019, 40(12): 1701-1704. doi: 10.3969/j.issn.1000-7369.2019.12.012
14	谢梅华, 吴虹. 葶苈大枣泻肺汤为主治疗小儿病毒性肺炎[J]. 湖北中医杂志, 2003, 25(6): 21. doi: 10.3969/j.issn.1000-0704.2003.06.012
15	段贤春, 黄石, 彭代银, 等. 网络药理学在中药复方研究中的应用[J]. 中国药理学通报, 2020, 36(3): 303-308. doi: 10.3969/j.issn.1001-1978.2020.03.003
16	GAN D H, XU X W, CHEN D Q, et al. Network pharmacology-based pharmacological mechanism of the Chinese medicine rhizoma drynariae against osteoporosis[J]. Medical Science Monitor, 2019, 25: 5700-5716. doi: 10.12659/MSM.915170
17	孟凡翠, 汤立达. 中药网络药理学研究中存在的问题与发展展望[J]. 中草药, 2020, 51(8): 2232-2237. doi: 10.7501/j.issn.0253-2670.2020.08.034
18	LOPES R D, MACEDO A V S, DE BARROS E SILVA P G M, et al. Continuing versus suspending angiotensin-converting enzyme inhibitors and angiotensin receptor blockers: Impact on adverse outcomes in hospitalized patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2):The BRACE CORONA Trial[J]. American Heart Journal, 2020, 226: 49-59. doi: 10.1016/j.ahj.2020.05.002
19	WILLCOX M D, WALSH K, NICHOLS J J, et al. The ocular surface, coronaviruses and COVID-19[J]. Clinical and Experimental Optometry, 2020, 103(4): 418-424. doi: 10.1111/cxo.13088
20	张勇刚, 张华茹, 李海明, 等. 还原型谷胱甘肽对急性加重期慢性阻塞性肺疾病患者TNF-α、IL-6和IL-8的影响[J]. 中国老年学杂志, 2011, 31(24): 4886-4887. doi: 10.3969/j.issn.1005-9202.2011.24.065
21	焦莹莹, 林子琦, 姚放鸣, 等. 新型冠状病毒肺炎与温疫关系的探讨[J]. 广东药科大学学报, 2020, 36(2): 169-173. doi: 10.16809/j.cnki.2096-3653.2020031002
22	陈瑞, 王玉光, 罗亚萍, 等. 新型冠状病毒肺炎中医证治初探[J]. 河北中医药学报, 2020, 35(2): 52-54. doi: 10.16370/j.cnki.13-1214/r.2020.02.016
23	姜师立. 重读传染病学专著《瘟疫论》[J]. 新阅读, 2020(4): 25-26.
24	黎志远. 葶苈大枣泻肺汤在肺系疾病中的应用[J]. 新中医, 1989(12): 16-17. doi: 10.13457/j.cnki.jncm.1989.12.011
25	吴国泰, 何小飞, 牛亭惠, 等. 大枣的化学成分、药理及应用[J]. 中国果菜, 2016, 36(10): 25-28. doi: 10.3969/j.issn.1008-1038.2016.10.007
26	马纳, 李亚静, 范吉平. 槲皮素药理作用研究进展[J]. 辽宁中医药大学学报, 2018, 20(8): 221-224. doi: 10.13194/j.issn.1673-842x.2018.08.061
27	雷晓青, 陈鳌, 刘毅, 等. 山萘酚药理作用的研究进展[J]. 微量元素与健康研究, 2017, 34(2): 61-62.
28	付成瑞. 山奈酚对小鼠放射性肺损伤防治作用的实验研究[D]. 济南: 济南大学, 2014.
29	刘悦, 任百洁, 邹馨颖, 等. 小檗碱的药理作用及有效药物载体的研究进展[J/OL]. 海南医学院学报(2020-04-09)[2020-04-28]. https://doi.org/10.13210/j.cnki.jhmu.20200408.006. doi: 10.13210/j.cnki.jhmu.20200408.006
30	张朝晖, 徐小云, 瞿星光, 等. 葶苈子水提液对内毒素致急性肺损伤大鼠肺泡Ⅱ型上皮细胞水通道蛋白5表达的影响[J]. 中国中医急症, 2016, 25(4): 606-608. doi: 10.3969/j.issn.1004-745X.2016.04.012
31	周文荣. 葶苈大枣泻肺汤合己椒苈黄丸加减治疗胸腔积液[J]. 浙江中西医结合杂志, 2011, 21(1): 22, 39. doi: 10.3969/j.issn.1005-4561.2011.01.010
32	郭盛, 唐于平, 段金廒, 大枣的化学成分及药理作用研究进展[C]// 全国第8届天然药物资源学术研讨会. 贵州:中国自然资源学会, 2008.
33	黄欢欢, 蔡成思, 黄育丹, 等. 五虎汤合葶苈大枣泻肺汤联合阿奇霉素对小儿支原体肺炎炎症因子与免疫功能的影响[J]. 中国药物与临床, 2018, 18(6): 1007-1009. doi: 10.11655/zgywylc2018.06.072
34	江振友, 周经姣, 方丹云, 等. SARS-CoV感染者血清IFN-γ和TNF-α的动态变化及其意义[J]. 免疫学杂志, 2004, 20(4): 285-287. doi: 10.3969/j.issn.1000-8861.2004.04.011
35	JIANG Z Y,ZHOU J J,FANG D Y,et al.Dynamic change of the IFN-γand TNF-αin serum of the patients with the SARS-coronavirus infection and its clinical significance[J]. Immunological Journal, 2004, 20(4): 285-287. doi: 10.3969/j.issn.1000-8861.2004.04.011
36	CHEN X, ZHOU S J, LI H. Evodiamine alleviates severe pneumonia induced by methicillin-susceptible Staphylococcus aureus following Cytomegalovirus reactivation through suppressing NF-κB and MAPKs[J]. International Journal of Molecular Medicine, 2018，42（6）:3247-3255. doi: 10.3892/ijmm.2018.3929
37	YU Y J, ZHANG Y, WANG S Y, et al. Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways[J]. Virology Journal, 2019, 16(1): 1-16. doi: 10.1186/s12985-019-1266-x
38	白鹏, 韩桂珍, 鲁丽. 支气管哮喘患儿痰液中CXCR2、CCR1、CCL3、CCL2表达及与肺功能和气道炎症的关系[J]. 河北医药, 2019, 41(23): 3555-3558. doi: 10.3969/j.issn.1002-7386.2019.23.008
39	TIAN S F, HU W D, NIU L, et al. Pulmonary pathology of early-phase 2019 novel coronavirus (COVID-19) pneumonia in two patients with lung cancer[J]. Journal of Thoracic Oncology, 2020, 15(5): 700-704. doi: 10.1016/j.jtho.2020.02.010
40	何黎黎, 龚普阳, 封玥, 等. 中药在抗新型冠状病毒肺炎(COVID-19)引起的细胞因子风暴中的应用分析[J]. 中草药, 2020, 51(6): 1375-1385. doi: 10.7501/j.issn.0253-2670.2020.06.002
41	LIU Q, ZHOU Y H, YANG Z Q. The cytokine storm of severe influenza and development of immunomodulatory therapy[J]. Cellularand Molecular Immunology, 2016, 13(1): 3-10. doi: 10.1038/cmi.2015.74
42	陈智, 朱海红, 杨英. 细胞因子风暴与肝衰竭[J]. 临床肝胆病杂志, 2014, 30(10): 981-983. doi: 10.3969/j.issn.1001-5256.2014.10.003
43	邵丽. TH17/IL-17在气道炎症疾病中的研究进展[D]. 蚌埠: 蚌埠医学院, 2014.
44	张竞文, 胡欣, 金鹏飞. 新型冠状病毒引起的细胞因子风暴及其药物治疗[J]. 中国药学杂志,2020,55(5):333-336. doi: 10.11669/cpj.2020.05.001
45	中国科学技术大学. 认识“炎症风暴”，推动有效阻断[J]. 国际人才交流, 2020(3): 36-37.
46	CAUVI D M, WILLIAMS M R, BERMUDEZ J A, et al. Elevated of IL-23/IL-17 pathway-related mediators correlates with exacerbation of pulmonary inflammation during polymicrobial Sepsis[J]. Shock, 2014, 42(3): 246-255. doi: 10.1097/SHK.0000000000000207
47	MA C B, ZU X Y, LIU K D, et al. Knockdown of pyruvate kinase M inhibits cell growth and migration by reducing NF-kB activity in triple-negative breast cancer cells[J]. Molecules and Cells, 2019, 42(9): 628-636. doi: 10.14348/molcells.2019.0038
48	刘双, 王雪峰, 郝欧美. 清肺通络膏对Ⅳ性肺炎大鼠肺组织PI3K/AKT/NF-KBP65蛋白表达的影响[J]. 中华中医药学刊, 2017, 35(9): 2289-2291. doi: 10.13193/j.issn.1673-7717.2017.09.023
49	YOUSEFI B, VALIZADEH S, GHAFFARI H, et al. A global treatments for coronaviruses including COVID-19[J/OL]. Journal of Cellular Physiology, 2020:29785. https://doi.org/10.1002/jcp.29785. doi: 10.1002/jcp.29785

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Study on the mechanism of Tingli Dazao Xiefei decoction in the treatment of COVID-19 based on the network pharmacology

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