Abstract: To analyze the mechanism of Tingli Dazao Xiefei decoction in the treatment of COVID-19,TCMSP database was used to screen the main active compounds and related targets,GeneCards database was employed to obtain the targets related with COVID-19,STRING and Cytoscape were used to get the PPI network analysis,Metascape database was applied to predict GO enrichment and KEGG pathway annotation, and Autodock was adopted for molecular docking. According to the results, there were 27 active components involved in Tingli Dazao Xiefei decoction and 39 targets aimed to COVID-19, including quercetin, kaempferol, etc., core targets such as TNF,MAPK1/8,CASP3. There were 146 KEGG pathways, from which IL-17, NF-κB, tuberculosis got the higher P value. The results of molecular docking showed that the core compounds quercetin, kaempferol, isorhamnetin and β-sitosterol of Tingli Dazao Xiefei decoction had a good affinity with ACE2. This study showed that quercetin, kaempferol, isorhamnetin of Tingli Dazao Xiefei decoction combined with ACE2, may participate in the IL-17 and NF-κB signaling pathway by regulating TNF, CASP3, MAPK, so as to inhibit cytokine storm and inflammatory injury, which could be applied to prevent COVID-19 by multi-component, multi-target and multi-pathway.

Key words: Tingli Dazao Xiefei decoction, COVID-19, network pharmacology, molecular docking