白藜芦醇对阿司咪唑诱导斑马鱼心脏损伤的保护作用

doi:10.3976/j.issn.1002-4026.2015.03.006

Abstract

Abstract: We explore the protection effect of different concentrations resveratrol to astemizoleinduced cardiac injury of zebrafish embryos.We treat zebrafish embryos with 4 μmol/L astemizole and different concentrations resveratrol with normal growth 48 hpf zebrafish embryo as an experimental model.We observe cardiac phenotypes, heart rates and the variation of SVBA at 72 hpf. Astemizole group shows heart injury such as pericardium edema, blood cell accumulation around the heart, heart rate reduction and SVBA interval shortening after drug feeding. However, astemizoleinduced heart toxicity significantly decreases with the increase of resveratrol concentration for resveratrol group.We therefore think resveratrol can protect astemizoleinduced cardiac injury of zebrafish embryos.

Key words: cardiac injury, heart rate, astemizole, resveratrol, protection, zebrafish

CLC Number:

R965.1

XUE Di, HAN Liwen, YANG Jifang, HE Qiuxia,WANG Xue,HOU Hairong，ZHANG Aiping, LIU Kechun. Protection effect of resveratrol to astemizoleinduced cardiac injury of zebrafish embryos[J].SHANDONG SCIENCE, 2015, 28(3): 29-33.

References

［1］朱俊靖, 陈汝家, 张勇, 等. 斑马鱼模型在药物毒性与安全性评价中的应用［C］// 华东地区第十二届实验动物科学学术交流会.宁波，2012. ［2］HE J H, GAO J M, HUANG C J, et al. Zebrafish models for assessing developmental and reproductive toxicity［J］. Neurotoxicology and Teratology, 2014, 42: 35-42. ［3］PARNG C. In vivo zebrafish assays for toxicity testing［J］. Current Opinion in Drug Discovery & Development, 2005, 8(1): 100-106. ［4］LEE E H, OH J H, PARK H J, et al. Simultaneous gene expression signature of heart and peripheral blood mononuclear cells in astemizoletreated rats［J］. Archives of Toxicology, 2010, 84(8): 609-618. ［5］王君, 胡霞敏, 曾繁典, 等. 阿司咪唑上市后心脏毒性安全性评价文献调研［J］. 药物流行病学杂志, 2009, 18(2): 85-87. ［6］郑卫红, 明章银, 胡本容, 等. 阿司咪唑对大鼠离体心脏心电图的影响［J］. 中国临床药理学与治疗学, 2007, 12(6): 682-685. ［7］李亚静, 王保玉. 白藜芦醇的研究进展［J］. 云南化工,2010,37（5）: 70-74. ［8］熊小春, 安春妹. 白藜芦醇抗肿瘤的研究进展［J］. 黑龙江医药,2010,23（1）:59-61. ［9］DAS S, CORDIS G A, MAULIK N, et al. Pharmacological preconditioning with resveratrol: Role of CREBdependent Bcl2 signaling via adenosine A3 receptor activation［J］. American Journal of PhysiologyHeart and Circulatory Physiology, 2005, 288(1): H328-H335. ［10］HATTORI R, OTANI H, MAULIK N, et al. Pharmacological preconditioning with resveratrol:Role of nitric oxide［J］. American Journal of PhysiologyHeart and Circulatory Physiology, 2002, 282(6): H1988-H1995. ［11］孙桂金, 潘杰, 刘可春, 等. 苯硫脲对斑马鱼黑色素生成及早期发育的影响［J］. 水产科学, 2011, 30(7): 387-390. ［12］吴晓敏, 何秋霞, 韩利文, 等. 阿司咪唑对斑马鱼心脏毒性的初步研究［J］. 中国药理学通报, 2013, 29(9): 1251-1254. ［13］MITTELSTADT S W, HEMENWAY C L, CRAIG M P, et al. Evaluation of zebrafish embryos as a model for assessing inhibition of hERG［J］. Journal of Pharmacological and Toxicological Methods, 2008, 57(2): 100-105.

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Protection effect of resveratrol to astemizoleinduced cardiac injury of zebrafish embryos

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