Abstract: This study is aimed to investigate the applicability of zebrafish thrombosis model in the screening of active ingredients of traditional Chinese medicine. In vitro platelet agglutination experiments and in vivo zebrafish thrombosis experiments were performed to detect the effects of monomers in traditional Chinese medicine material on platelet aggregation and thrombosis. We selected a 3~5-days-old transgenic TG line (integrin αIIb: enhanced green fluorescent protein) of platelet-labeled green fluorescent zebrafish and wild-type AB line zebrafish as screening models and used ferric chloride to induce thrombosis. The zebrafish were treated with 20 types of monomer components of traditional Chinese material, and clotting time was used as an indicator to investigate the effect of these samples on thrombosis in zebrafish. Simultaneously, the whole blood of rats was collected to prepare platelet-rich plasma and platelet-poor plasma, and platelet aggregation rate was measured by a platelet agglutination instrument to detect the activity of monomer components of Chinese medicine material. There were seven types of monomer components of traditional Chinese medicine material demonstrated antithrombotic effects in zebrafish. For the first time, it was first found that aucubin had a significant inhibitory effect on thrombosis in zebrafish. The results of in vitro platelet aggregation test showed that 12 traditional Chinese medicine components inhibited platelet aggregation, of which the maximum inhibition of platelet aggregation (88.22%) was exhibited by 100 μg/mL salidroside. The seven types of monomer components of traditional Chinese medicine material found to have antithrombotic effects in zebrafish model exhibited good results in in vitro platelet aggregation experiments as well. The zebrafish thrombosis model has good adaptability to the screening of antithrombotic activity of traditional Chinese medicine.

Key words: zebrafish, thrombosis, traditional Chinese medicine, drug screening, lead compound