Abstract:

 We employed the dying method of Coomassie brilliant blue G-250 to determine the protein content of milk, measuring the absorbency at the wavelength of 595 nm. Experiment shows that a good linear relationship exists between the absorbency and the content of standard proteins in the range of 10~80 μg/mL. This approach has such advantages as simplicity, rapidity, good reproducibility and sensitivity, especially suitable for the determination of milk protein.

Key words: milk, content of proteins, Coomassie brilliant blue dying method, determination