HPLC法测定乙肝扶正清毒颗粒中芍药苷的含量

doi:10.3976/j.issn.1002-4026.2019.02.004

Abstract

Abstract: A method for the determination of paeoniflorin content in Yiganfuzhengqingdu Granules by HPLC was established. HPLC analysis was carried out with Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm，5 μm) and methanol-0.05 mol/L potassium dihydrogen phosphate(38:62, V/V) as mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 25 ℃,injection volume was 10 μL, the detection wavelength was 230 nm. The results showed that the calibration curve was linear in the range of 0.079 1～0.988 0 μg for paeoniflorin (r=0.999 9), the recovery rate was 98.1%~100.6% with RSD 1.02%(n=6). This method is highly specific, sensitive, reproducible and simple, which can be used for quality control of Yiganfuzhengqingdu Granules.

Key words: Yiganfuzhengqingdu Granules, paeoniflorin, HPLC, content determination

CLC Number:

R284.2

LI Yan, CHENG Hong-jie, LI Ke-ming. Content determination of paeoniflorin in Yiganfuzhengqingdu Granules by HPLC[J].Shandong Science, 2019, 32(2): 22-27.

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Content determination of paeoniflorin in Yiganfuzhengqingdu Granules by HPLC

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