Abstract:

A method was established to determine the content of Eugenol and paeoniflorin in Dingbai plaster by HPLC. The analysis was performed on Lichrospher C18(4.6 mm×150 mm, 5 μm) column, with the mobile phase of methanolwater (52:48,V/V). At a flow rate of 1 mL·min-1, the detection wavelength was set at 280 nm. The column temperature was kept at 30 ℃, and the content of eugenol in preparation was determined; with the mobile phase of acetonitrilewater (13:87,V/V)，at a flow rate of 1 mL·min-1，the detection wavelength was set at 230 nm, the column temperature was kept at 30 ℃, and the content of paeoniflorin in preparation was determined. Better linear relationship exists in the range 0.047 2～0.472 μg (R=1.000 0) for eugenol. And in the range 0.054 6～0.491 4 μg (R=0.999 9) for paeoniflorin. The method is simple, reproducible, accurate and reliable. It can be used as a method for the determination of the content of Dingbai plaster.

Key words: eugenol, high performance liquid chromatography, content determination, paeoniflorin, Dingbai plaster