海洋深层水与褐藻糖胶配伍应用抑制HepG2肝细胞脂质积聚调节作用的研究

doi:10.3976/j.issn.1002-4026.2015.06.003

Abstract

Abstract: We addressed regulation effect of matched application of deep sea water (DSW) and fucoidan (FPS) on lipid metabolism with HepG2 cells as models. We confirmed the concentration of FPS matched with DSW should be controlled within 50 μg/mL by MTT assay. Within this scope, lipid content of HepG2 cells gradually decreased with the increase of FPS concentration (5, 10, 20, and 50 μg/mL). Significant doze dependent phenomenon exists. Moreover, matched application of DSW and 50 μg/mL FPS was significantly superior to single 50 μg/mL DSW or FPS.Experimental results show that matched application of DSW and FPS has better regulation effect to lipid metabolism of HepG2 cells, optimal matched application concentration of 50 μg/mL. This research provides a new solution for prevention and treatment of hyperlipidemia.

Key words: deepsea water, HepG2 cells, fucoidan, matched application, lipid accumulation

CLC Number:

R965.1

He Shan, Peng Weibing, Zhou Honglei,Liu Kechun,Xia Xuan. atched application of deep sea water and fucoidin in attenuation of lipid accumulation in HepG2 cells[J].SHANDONG SCIENCE, 2015, 28(6): 11-16.

References

［1］TSUCHIYA Y, NAKAMURA K, SEKIKAWA H, et al, Yamamoto M. Subacute effects of deepsea water from the Japan Sea on blood examination values in mice［J］. Environ Health Prev Med, 2002,7(5):189-192. ［2］ TSUCHIYA Y, WATANABE A, FUJISAWA N, et al. Effects of desalted deep seawater on hematologic and blood chemical values in mice［J］. Tohoku J Exp Med, 2004,203(3):175-182. ［3］何珊,李春霞,管华诗.海洋深层水的药理活性研究进展［J］.中国海洋药物,2011,30(1):65-68. ［4］YOSHIOKA S, HAMADA A, CUI T, et al. Pharmacological activity of deepsea water: examination of hyperlipemia prevention and medical treatment effect［J］. Biol Pharm Bull, 2003,26(11):1552-1559. ［5］ MIYAMURA M, YOSHIOKA S, HAMADA A, et al. Difference between deep seawater and surface seawater in the preventive effect of atherosclerosis［J］. Biol Pharm Bull, 2004 ,27(11):1784-1787. ［6］ KIMURA M. The effects on health status by drinking water made from deep sea water without salt-The decline effect on plasma cholesterol by drinking water made from deep sea water without salt［J］. Magnesium Research, 2004, 23 (2): 79-86. ［7］ HE S, HAO J J, PENG W B, et al. Modulation of lipid metabolism by deepsea water in cultured human liver (HepG2) cells［J］. Marine Biotechnology,2014,16(2): 219-229. ［8］ FU Z Y, YANG F L, HSU H W, et al. Drinking deep seawater decreases serum total and lowdensity lipoproteincholesterol in hypercholesterolemic subjects［J］.J Med Food, 2012, 15(6):535-541. ［9］ CHEVOLOT L,MULLOY B, RATISKOL J, et al. A disaccharide repeat unit is the major structure in fucoidans from two species of brown algae［J］.Carohydr Res,2001,330(4):529-535. ［10］ HUANG L, WEN K, GAO X, et al. Hypolipidemic effect of fucoidan from Laminaria japonica in hyperlipidemic rats［J］. Pharm Biol,2010,48(4):422-426. ［11］邢燕红.褐藻糖胶（FPS）的调脂机制探讨［D］. 广州:广州中医药大学,2005. ［12］ VIOLLET B, FORETZ M, GUIGAS B, et al. Activation of AMPactivated protein kinase in the liver:A new strategy for the management of metabolic hepatic disorders［J］. J Physiol, 2006, 574(1):41-53. ［13］ JAVITT N B. HepG2 cells as a resource for metabolic studies:Lipoprotein, cholesterol and bile acids［J］.FASEB J,1990,4(2):161-168. ［14］何珊.海洋深层水对代谢综合征预防作用的初步研究［D］.青岛：中国海洋大学,2012. ［15］王晶.海带褐藻多糖硫酸酯的结构、化学修饰与生物活性研究［D］.青岛：中国科学院海洋研究所,2010. ［16］ ALESIANI D, CANINI A, ABROSEA B D, et al. Antioxidant and antipro1iferative activitiesc of phytochemicals from Quince (Cydonia vulgaris) peels［J］. Food Chemistry,2009,118(2):199-207. ［17］ LIAN Z, LI Y, GAO J, et al. A novel AMPK activator, WS070117,improves lipid metabolism discords in hamsters and HepG2 cells［J］. Lipids Health Dis. 2011,10(2):67-75. ［18］张永欢, 李枝杰.高脂血症的中西医治疗［M］.上海:上海医药大学出版社.2001:2-5. ［19］ JAIN K S, KATHIRAVAN M K, SOMANI R S, et al. The biology and chemistry of hyperlipidemia［J］.Bioorg Med Chem,2007, 15(14):4674-4699. ［20］张蔚. 他汀类药物治疗高脂血症的临床应用［J］.新医学,2007,38(5):330-331.

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atched application of deep sea water and fucoidin in attenuation of lipid accumulation in HepG2 cells

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