利用斑马鱼模型评价氨基葡萄糖盐酸盐及淫羊藿的促进骨骼矿化作用

doi:10.3976/j.issn.1002-4026.2016.02.005

Abstract

Abstract:

We addressed the activity of bone mineralization promotion of different concentrations of glucosamine hydrochloride and epimedium extract based on zebrafish model. We selected 72 hpf normal growth zebrafish embryos as subjects, and cultivation water as control group or different concentrations (1, 10 and 100 μg/mL) of glucosamine hydrochloride solutions or epimedium extract solutions as experiment group. After fourday cultivation, all the zebrafish was dyed for 1 h with 0.2% calcein. We then observed their skeleton growth with a microscope, and calculated its stained area through an image analysis software. Results indicate that glucosamine hydrochloride and epimedium extract of all experiment groups all have some promotion effect on skeleton mineralization of zebrafish, as compared with control group, obvious dose dependence. This shows that both glucosamine hydrochloride and epimedium extract have significant bone mineralization activity to zebrafish. Moreover, glucosamine hydrochloride has better mineralization activity than epimedium extract for the same concentration.

Key words: zebrafish, glucosamine hydrochloride, bone mineralization, epimedium extract

CLC Number:

R965.1

ZHANG Shanshan, HE Qiuxia, HAN Jian, WANG Xue, HOU Hairong, LI Xiaobin,ZHANG Xuanming, HAN Liwen*, LIU Kechun*. Evaluation of bone mineralization promotion of glucosamine hydrochloride and epimedium extract with zebrafish model[J].SHANDONG SCIENCE, 2016, 29(2): 19-24.

References

［1］雷署丰.中国人群骨密度的四个候选基因和相关因素研究［D］.长沙：湖南师范大学，2003.
［2］王文娇.骨质疏松患者骨密度与骨质疏松危险因素的相关性研究［D］.太原：山西医科大学，2015.
［3］KARI G, RODECK U, DOCLER A P. Zebrafish:An emerging model system for human disease and drug discovery ［J］. Clinical Pharmacology & Therapeutics, 2007, 82(1):70-80.
［4］PENBERTHY W T, SHAFIZADEH E, LIN S. The zebrafish as a model for human disease ［J］. Front Biosci, 2002, 7:1439-1453.
［5］李淼. 地塞米松磷酸钠导致斑马鱼颅面部发育异常的研究［D］. 武汉：华中科技大学，2009.
［6］FLEMING A，SATO M,GOLDSMITH P. Highthroughput in vivo screening for bone anabolic compounds with zebrafish ［J］.Journal of Biomolecular Screening,2006,10(8):823-831.
［7］ LI N, FELBER K, ELKS P, et al. Tracking gene expression during zebrafish osteoblast differentiation ［J］. Dev Dyn, 2009, 238(3):459-466.
［8］黄紫洋.甲壳素及其衍生物的制备与性质的研究［D］.福州：福建师范大学.2002:15-19.
［9］邱俊生，曹正洪，吴九妹.一种具有增加骨密度功能的药物组合物及其应用：中国，102657842A［P］.2012-09-12.
［10］蒲渝，张怡五，陈永龙，等. 盐酸氨基葡萄糖辅助治疗Pilon骨折的临床研究［J］. 华西医学，2013, 28(3):352-355.
［11］王大利，耿成燕，姜丽萍，等. 关节软骨及骨破坏模型大鼠经盐酸氨基葡萄糖干预的最佳剂量［J］. 中国组织工程研究与临床康复,2011, 15(28):5145-5148.
［12］柯素霞，牛海明，刘启旺.盐酸氨基葡萄糖治疗65例骨关节炎疗效观察［J］.大家健康：学术版，2014, 8(11):176.
［13］王忠平.盐酸氨基葡萄糖治疗骨关节炎的疗效评价［J］. 华西医学，2013, 28(1):85-86.
［14］王建彬.盐酸氨基葡萄糖治疗骨关节炎的临床分析［J］.中国卫生标准管理，2015（1）：169-170.
［15］崔红霞,王卫卫,孟丽华.盐酸氨基葡萄糖治疗骨关节炎的临床观察［J］.中国实用医药，2008, 3(34):163-164.
［16］金丽，杨月琴，王松.淫羊藿对RANKL、MCSF诱导的破骨样细胞形成及FN表达的影响［J］.华中师范大学学报：自然科学版，2009, 43(4):640-644.
［17］王建忠，高鸿雁，王坤正，等.淫羊藿对激素性股骨头坏死骨组织OPG/RANKL mRNA表达的影响［J］.南方医科大学学报，2011, 31(10):1714-1717.
［18］李盛林，高华，章魁华，等.淫羊藿对小鼠成骨细胞增殖、功能及凋亡的影响［J］.北京大学学报：医学版，2008, 40(1):43-46.
［19］彭维兵，韩建，陈维云，等.昆布多糖对氯化镉诱导斑马鱼骨骼发育毒性的改善作用［J］. 山东科学，2015,28(5):1002-4026.
［20］韩利文，刘可春，蔡志杰，等. 一种影响骨骼矿化作用药物的筛选系统:中国,103063630A ［P］ .2013-04-24.

[1]	FAN Wei, SHEN Chuanlin, ZHANG Xuanming, DU Xingshuo, ZHAN Wen, SUN Chen, JIN Meng, LI Xiaobin, ZHANG Sichen, SUN Botong, HE Qiuxia. Prediction of anti-aging mechanism of Panax quinquefolius L. by network pharmacology and molecular docking [J]. Shandong Science, 2024, 37(6): 42-50.
[2]	CHEN Shanjun, WANG Huan, HU Kaiqing, BI Wenjie, CHENG Guidong, WANG Songsong, HAN Liwen, WANG Xiaojing. Screening of potential antiepileptic active ingredients in Rhizoma Gastrodiae based on zebrafish model and metabolomics technology [J]. Shandong Science, 2024, 37(3): 1-9.
[3]	MA Shijing, HE Chunyan, GUAN Tianzhu, YAO Xueshuang, ZHANG Junpeng. Exploration of antihyperlipidemia mechanism of Monopterus albus peptides based on hyperlipidemic zebrafish model and network pharmacology [J]. Shandong Science, 2024, 37(3): 27-38.
[4]	LIU Kechun, WANG Yongcheng, ZANG Xiaohan, XIA Qing, ZHANG Yun, ZHANG Shanshan, SUN Chen. Advancements in network pharmacology and zebrafish modeling for studying traditional Chinese medicine’s effective substances and mechanisms of action [J]. Shandong Science, 2024, 37(2): 29-35.
[5]	XIA Qing, ZANG Xiaohan, WANG Yongcheng, ZHANG Yun, LI Peihai, ZHANG Xuanming, LIU Kechun. Progress and development trends in the use of zebrafish as a model organism for evaluating cosmetic efficacy [J]. Shandong Science, 2024, 37(2): 36-46.
[6]	WU Yonghao, XIE Hebing, HOU Hairong, WANG Rongchun, CHEN Xiqiang. Protective effect of Feirepuqing Powder on hypoxia-induced sports injury in zebrafish model by inhibiting Hif1α [J]. Shandong Science, 2024, 37(1): 32-38.
[7]	SHI Ruidie, GAO Xin, WANG Baokun, GAO Daili, JIN Meng, ZHANG Xiujun. Anti-Alzheimer's disease activity of oroxin A and its mechanism of action based on zebrafish model [J]. Shandong Science, 2023, 36(6): 28-37.
[8]	ZOU Hong-yuan, ZHU Cheng-yue, ZHANG Shan-shan, KONG Rui-qi, ZHANG Yun, LIU Ke-chun. Protective effect of peony seed oil microcapsule powder on isoniazid-induced developmental toxicity in zebrafish [J]. Shandong Science, 2021, 34(4): 45-51.
[9]	MO Cai-lian, LI Jie, WANG Jia-zhen, LIU Xin , LIN Sheng-hua, MOU Yan-ling, ZHANG Yun, LIU Ke-chun, HE Qiu-xia. Applicability of a zebrafish thrombosis model in screening active ingredients of traditional Chinese medicine [J]. Shandong Science, 2021, 34(4): 52-59.
[10]	LIU Ke-chun, GAO Yan, ZHANG Yun, HE Qiu-xia, HAN Li-wen, LI Ning, JI Xiu-na, SUN Chen. Zebrafish as a model organism for hearing research [J]. Shandong Science, 2019, 32(5): 46-53.
[11]	WANG Rong-chun，HE Qiu-xia，HAN Jian，CHEN Xi-qiang，SUN Chen，WANG Xi-min，HAN Li-wen，LIU Ke-chun. Research on the effects of chloramphenicol on developmental and immune toxicity of juvenile zebrafish [J]. SHANDONG SCIENCE, 2017, 30(6): 35-40.
[12]	LIU Fa-sheng, WANG Rong-chun, GUO Jing-lan, CHEN Yan-li, XIA Qing, KONG Hao-tian, FENG Run-liang, HAN Li-wen, ZHANG Ai-ping, LIU Ke-chun. The immune-regulatory effects of active ingredients of asparagus using zebrafish model [J]. SHANDONG SCIENCE, 2017, 30(6): 29-34.
[13]	PENG Wei-bing, ZHOU Ling-xiao, FU Xian-jun, HE Qiu-xia, WANG Zhen-guo, LIU Ke-chun. Anti-inflammatory research on component combination of Kunhai ginger decoction based on zebrafish model [J]. SHANDONG SCIENCE, 2017, 30(2): 37-42.
[14]	SUN Chen, HE Qiu-xia, HAN Li-wen, CHEN Xi-qiang, WANG Xi-min, CHU Jie, HAN Jian, WANG Rong-chun, HOU Hai-rong, LIU Ke-chun. Toxicity of aspirin to gastrointestinal tract of zebrafish [J]. SHANDONG SCIENCE, 2016, 29(6): 50-55.
[15]	HOU Hai-rong,ZHANG Shan-shan,SUN Chen, CHEN Yu-feng, HE Qiu-xia,LI Xiao-bin, YU Tao, PENG De-jie, HAN Li-wen, LIU Ke-chun. The effect of fructo oligosaccharides and isomaltooligosaccharide on the intestinal peristalsis of zebrafish [J]. SHANDONG SCIENCE, 2016, 29(6): 56-61.

Evaluation of bone mineralization promotion of glucosamine hydrochloride and epimedium extract with zebrafish model

PDF (PC)

Like

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0