Abstract: We addressed recovery effect of different concentrations of laminarin on developmental toxicity of zebrafish skeleton induced by cadmium chloride. We selected 72 hpf normal growth zebrafish embryos, and added 100 μg/L of cadmium chloride and 25, 50, 100 μg/L of laminarin. Three days later, they were dyed for 2 h by calcein. We observed skeletal growth of zebrafish on microscope, and calculated the fluorescent staining area of hard bone. Results show that different concentrations of laminarin can all reduce the toxicity of zebrafish skeletal growth induced by cadmium chloride, and dosedependent relation exists. Laminarin of 100 μg/L has significant recovery effect, but the effect is the worst for laminarin of 25 μg/L. This shows that laminarin has significant recovery effect on cadmium chloride induced zebrafish skeletal developmental toxicity.

Key words: laminarin, toxicity, zebrafish, skeletal growth, cadmium chloride