Abstract: We develop a determination method for cafeic acid,ferulic acid, isoferulic acid in Cimicifugae Rhizoma with HPLC and address the impact of honeyfired processing on organic acid in Cimicifugae Rhizoma.We employed Thermo AcclaimTM120 C18 column(4.6 mm×250 mm,5 μm）to perform gradient elution with acetonitrile0.1％phosphoric acid as mobile phase, flow rate of 1.0 mL/min, column temperature of 25 ℃, and detection wavelength of 316 nm.Results show that better linear relationship exists in 0~0.028 μg for cafeic acid（R＝0.999 5）, 0~0.067 μg for ferulic acid（R＝0.999 4）and 0~0.27 μg for isoferulic acid（R＝0.999 9）.Their recovery rates are 99.8％,99.4% and 101.6%.The method is simple,reliable and accurate and can be used for proving some influences of honeyfired processing on the three organic acid in Cimicifugae Rhizoma.

Key words: Cimicifugae Rhizoma, cafeic acid, ferulic acid, isoferulic acid, honeyfired processing