基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

doi:10.3976/j.issn.1002-4026.20240129

Abstract

Abstract: To investigate the metabolic differences among different parts of Lonicera japonica Thunb.,a nontargeted metabolomics study was conducted on its stems, leaves, and flowers using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).Intotal,773 metabolites in positive-ion mode and 572 and 572 metabolites in negative-ion mode were identified in samples from different parts of L. japonica Thunb.; 553 differential metabolites were detected by comparing leaves and flowers; and 471 differential metabolites were detected by comparing stems and flowers, mainly phenolic acids, amino acids and their derivatives, flavonoids, and isoflavones. The pathways related to differential metabolites mainly include phenylpropanoid biosynthesis, flavonoid and flavonol biosynthesis, purine metabolism, and anthocyanin biosynthesis. This study analyzed the predominant compounds and important metabolic pathways involved in different parts ofL. japonica Thunb.,i.e.,the stems,leaves, and flowers,comparing them with medically used flowers. The findings provides a scientific basis for the further development and utilization ofL. japonica Thunb.resources.

Key words: nontargeted metabolomics, Lonicera japonica Thunb., differential metabolites, metabolic pathway

CLC Number:

R284.1

ZHANG Xuxin, ZHANG Luzhe, LIN Shuqian, ZHAO Zengcheng, MENG Zhaoqing, DONG Xiaodi, CAO Guiyun, HE Jixiang, WANG Daijie. Analysis of differential metabolites in different parts of Lonicera japonicaThunb.based on non-targeted metabolomics technology[J].Shandong Science, 0, (): 1-.

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Analysis of differential metabolites in different parts of Lonicera japonicaThunb.based on non-targeted metabolomics technology

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