基于非靶向代谢组学技术的忍冬不同部位差异代谢物分析

doi:10.3976/j.issn.1002-4026.20240129

Abstract

Abstract:

To investigate the metabolic differences among different parts of Lonicera japonica Thunb.,a nontargeted metabolomics study was conducted on its stems, leaves, and flowers using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).Intotal,773 metabolites in positive-ion mode and 572 and 572 metabolites in negative-ion mode were identified in samples from different parts of L. japonica Thunb.; 553 differential metabolites were detected by comparing leaves and flowers; and 471 differential metabolites were detected by comparing stems and flowers, mainly phenolic acids, amino acids and their derivatives, flavonoids, and isoflavones. The pathways related to differential metabolites mainly include phenylpropanoid biosynthesis, flavonoid and flavonol biosynthesis, purine metabolism, and anthocyanin biosynthesis. This study analyzed the predominant compounds and important metabolic pathways involved in different parts of L. japonica Thunb.,i.e.,the stems,leaves, and flowers,comparing them with medically used flowers. The findings provides a scientific basis for the further development and utilization of L. japonica Thunb. resources.

Key words: nontargeted metabolomics, Lonicera japonica Thunb., differential metabolites, metabolic pathway

CLC Number:

R284.1

ZHANG Xuxin, ZHANG Luzhe, LIN Shuqian, ZHAO Zengcheng, MENG Zhaoqing, DONG Xiaodi, CAO Guiyun, HE Jixiang, WANG Daijie. Analysis of differential metabolites in different parts of Lonicera japonica Thunb. based on non-targeted metabolomics technology[J].Shandong Science, 2025, 38(6): 29-41.

Figures/Tables 10

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References 41

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组别	优势代谢物种类	优势代谢物		KEGG代谢通路编号
花	苯丙烷类和聚酮类	木通苯乙醇苷B、蒙花苷		-
	核苷酸及衍生物	腺苷3'5'-环磷酸		map00230
	有机酸及其衍生物	苏氨酸		map00260 map00261 map00290 map00860 map00970 map01100 map01110 map01230 map02010
	有机杂环化合物	鸟嘌呤		map00230 map01100
	脂质和类脂分子	15-氧代EDE、鹅去氧胆酸、衣康酸		map01100 map00660
叶	苯丙烷类和聚酮类	6-甲基香豆素、7-去甲基软木花椒素		-
	苯环型化合物	β,β-二甲基丙烯酰阿卡宁、异香草酸		-
	核苷酸及衍生物	S-(5'-腺苷)-L-高半胱氨酸		-
	有机酸及其衍生物	2-氧代己二酸、D-苯丙氨酸、羽扇豆素A、柠檬酸三乙酯		map00300 map00310 map00380 map01100 map01210 map01230 map00360
	有机氧化合物	13,14-dihydro-15-keto-tetranor Prostaglandin D2		-
	有机杂环化合物	4-吡哆酸盐、古伦宾吲哚-3-丙酮酸、犬尿喹啉酸		map00750 map01100 map00380
	脂质和类脂分子	18-β-甘草次酸、土木香内酯		-
茎	苯丙烷类和聚酮类	(+)-儿茶素、槲皮素-3-O-β-吡喃葡萄糖基-6'-乙酸酯		map00941 map01110
茎	苯环型化合物	3-羟基萘-2,7-二磺酸		-
茎	有机酸及其衍生物	DL-蛋氨酸、L-苯丙氨酸 N-乙酰基-L-苯丙氨酸	map00941 map01110 map00360 map00400 map00460 map00940 map00960 map00966 map00970 map01100 map01110 map01210 map01230 map02010
茎	脂质和类脂分子	山楂酸、熊果酸	-

Analysis of differential metabolites in different parts of Lonicera japonica Thunb. based on non-targeted metabolomics technology

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代谢物类别	代谢物数量
代谢物类别	总量	花	叶	茎
脂质和类脂分子	305	277	263	272
苯丙烷类和聚酮类	197	154	175	167
有机氧化合物	105	96	95	100
有机酸及其衍生物	118	111	103	111
苯环型化合物	92	85	90	83
有机杂环化合物	89	83	84	75
核苷、核苷酸和类似物	37	34	27	34
木质素、新信号素和相关化合物	17	11	12	13
生物碱和衍生物	20	15	14	9
有机氮化合物	12	10	8	11
其他	353	308	302	301

代谢物类别	叶vs.花		茎vs.花		茎vs.叶
代谢物类别	含量增加数量	含量降低数量	含量增加数量	含量降低数量	含量增加数量	含量降低数量
脂质和类脂分子	23	24	13	22	32	48
苯丙烷类和聚酮类	23	10	21	6	19	26
有机氧化合物	13	7	5	4	5	10
有机酸及其衍生物	11	7	6	3	14	14
苯环型化合物	11	2	8	4	6	16
有机杂环化合物	10	6	1	9	7	18
核苷、核苷酸和类似物	2	5	2	2	4	4
木质素、新信号素和相关化合物	2	1	1	0	2	3
生物碱和衍生物	3	1	1	1	1	1
有机氮化合物	0	6	1	0	0	0
其他	47	31	24	27	27	43

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