十全大补膏中芍药苷的含量测定

doi:10.3976/j.issn.1002-4026.2017.05.005

Abstract

Abstract:

A HPLC method for the determination of paeoniflorin in tonic semifluid extract of ten ingredients was established. The HPLC analysis was performed on Kromasil C18 column (4.6 mm×250 mm, 5 μm), acetonitrilewater (17∶83, V/V) as the mobile phase, and the column temperature was 30 ℃, injection volume was 10 μL, the detective wavelength was at 230 nm, at a flow rate of 1.0 mL／min. Results revealed that paeoniflorin at the content of 0.083 6～0.627 0 μg showed good linearity（r= 0.999 8），the recovery rate was 98.98%～100.47%, RSD was 0.65% (n=6). This method is highly specific, sensitive, reproducible, and simple, which can be used for the quality control of this preparation.

Key words: HPLC, tonic semifluid extract of ten ingredients, paeoniflorin, content determination

CLC Number:

R284.1

LIU Qing, LIU Bo，DING Xiao-yan. Content determination of paeoniflorin in tonic semifluid extract of ten ingredients[J].SHANDONG SCIENCE, 2017, 30(5): 28-31.

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Content determination of paeoniflorin in tonic semifluid extract of ten ingredients

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