Abstract: To determine the optimal preparation method of polysaccharides with immune activity from Radix Glehniae, we employed four different approaches to prepare polysaccharides from Radix Glehniae, and performed the experiment of spleen lymphocytes of mouse proliferation test in vitro.We also compared immune activity of each polysaccharide sample. With extraction rate and proliferation rate of spleen lymphocytes of mouse as parameters, we analyzed the necessity of 95% ethanol reflux to remove small molecules, protein elimination and ethanol precipitation of different concentrations,which might determine optimal preparation method of polysaccharides with immune activity from Radix Glehniae. By comparisons of extraction rate and proliferation rate,the sample through processes of boiling water extraction, concentration contraction and desiccation has the highest extraction rate of 33.15% and the strongest proliferation rate of spleen lymphocytes of mouseof 33.93% at the concentration of 62.5 μg/mL.Experimental results show that optimal preparation method of polysaccharides with immune activity from Radix Glehniae is boiling water extraction, concentration contraction and desiccation, but not 95% ethanol reflux, protein elimination and ethanol precipitation.

Key words: polysaccharides from Radix Glehniae, immune activity, spleen lymphocytes proliferation of mouse