HPLC法测定首乌益智胶囊中大黄素和大黄素甲醚的含量

doi:10.3976/j.issn.1002-4026.2016.05.006

Abstract

Abstract:

We constructed a HPLC based content determination method for emodin and emodin monomenthyl ether from Shouwuyizhi capsule. Samples were separated by C18 (250 nm×4.6 nm, 5 μm) column with methanol0.1% phosphoric acid water (82:18, V/V) as mobile phase. Determination conditions were determination wavelength of 289 nm, flow rate of 1.0 mL/min, and column temperature of 30 ℃. Better linear relationship exists in the range 0.019~0.19 μg（R=0.999 9,n=5) for emodin, and in the range 0.021~0.21 μg（R=1.000 0，n=5）for emodin monomenthyl ether. Their average recovery rates are respectively 98.58% and 100.01%, and RSDs are 2.66% and 1.15%. Results show that the method has strong specificity and accuracy, so it can be applied to simultaneous content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule.

Key words: content determination, Shouwuyizhi capsule, emodin, HPLC, emodin monomenthyl ether

CLC Number:

R284.1

DING Xiaoyan, LIU Qing, JIA Yuanyin, ZHAO Bonian. HPLC based content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule[J].SHANDONG SCIENCE, 2016, 29(5): 36-40.

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HPLC based content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule

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