银杏叶中银杏内酯提取工艺研究

doi:10.3976/j.issn.1002-4026.2016.05.005

Abstract

Abstract:

We determined optimum extraction condition of Ginkgo biloba leaves with the extraction rate of ginkgolides (ginkgo lactone, ginkgolides A, ginkgolide B and ginkgolide C) as an index. We employed orthogonal experiment to systematically investigate the impact of extraction time, solidliquid ratio, ethanol concentration, and extraction time on extraction technology of Ginkgo biloba leaves. Ginkgolides content was determined through optimization of high performance liquid chromatography (HPLCELSD) and isopropanol of mobile phase. We also calculated extraction rate. Optimum extraction conditions are extraction time of 1.5 h, solidliquid ratio of 1：20, ethanol concentration of 50% and extraction times of 3. This technology can improve extraction rate of ginkgolides, save testing cost, and is applicable to industrial production.

Key words: extraction rate, Ginkgo biloba leaves, orthogonal experiment, isopropyl alcohol, ginkgolides

CLC Number:

R284.2

LI Wendong, GAO Qianshan,YANG Peng, YANG Bingtian,WANG Xiao. Extraction technology of ginkgolides from Ginkgo biloba leaves[J].SHANDONG SCIENCE, 2016, 29(5): 29-35.

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Extraction technology of ginkgolides from Ginkgo biloba leaves

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