Shandong Science

   

Screening, identification and functional evaluation of trichoderma strains antagonistic to strawberry anthracnose

LIU Jianhua1,JIANG Yanqing1,LI Hongmei1,Yang Jianwen2,WEI Yanli1,LIU Baojun1,LI Jishun1,HU Jindong1   

  1. 1.Ecology Institute ,Shandong Provincial Key Laboratory of Applied Microbiology,Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; 2.ShandongTenov Pesticide Co, Ltd., Weifang 262200, China
  • Received:2025-03-16 Accepted:2025-05-13 Published:2025-09-11 Online:2025-09-11

Abstract: In response to the severe incidence and management challenges of strawberry anthracnose, this study targeted Colletotrichum siamense, the pathogen responsible for the disease. We used dual culture and sealed-plate assays to screen for the effective biocontrol Trichoderma strain HB 23422, which exhibited strong antagonistic activity against strawberry anthracnose, and was identified based on morphological characteristics and molecular biology as Trichoderma asperellum. On PDA plates, HB 23422 exhibited a strong inhibitory effect on Colletotrichum Siamense CM9, with an inhibition rate of more than 74.55%. Vitro itro leaf experiments and pot experiments showed that the spore suspension of this strain could effectively reduce the incidence of strawberry anthracnose. The strain HB 23422 grows rapidly, produces abundant spores, and has excellent disease control efficacy, indicating its potential for development as microbial pesticide for environment friendly control of strawberry anthracnose.

Key words: Colletotrichum siamense, Trichoderma, Separation and identification, Inhibition rate

CLC Number: 

  • S182