哈茨木霉LTR-2对冬小麦籽粒的影响

doi:10.3976/j.issn.1002-4026.2021.06.008

Abstract

Abstract:

To explore the effect of Trichoderma harzianum LTR-2 on wheat grain quality and provide a practical method for improving wheat quality, parameters such as thousand seed weight as well as amino acid, protein, and wet gluten contents of winter wheat grains (cultivar Jimai 44) were analyzed after spraying different concentrations of T. harzianum LTR-2 suspension at the booting stage. The results demonstrate that the analyzed parameters had improved compared with the controls at the booting stage. The aforementioned results indicated that spraying of T. harzianum LTR-2 at the booting stage could improve the yield of wheat as well as nutritional and processing quality of wheat grain.

Key words: winter wheat, booting stage, T. harzianum LTR-2, thousand seed weight, protein, amino acid, wet gluten

CLC Number:

Q939.96

LI Ling, LIU Bao-jun, YANG Kai, CHEN Kai, WANG Yi-lian, LI Ji-shun. Effect of Trichoderma harzianum LTR-2 on winter wheat grain quality[J].Shandong Science, 2021, 34(6): 62-67.

Figures/Tables 4

Table 1

Table 2

Table 3

Table 4

References 26

[1]	ANJUM F M, AHMAD I, BUTT M S, et al. Amino acid composition of spring wheats and losses of lysine during chapati baking[J]. Journal of Food Composition and Analysis, 2005, 18:523-532. DOI: 10.1016/j.jfca.2004.04.009. doi: 10.1016/j.jfca.2004.04.009
[2]	BARNEIXA J. Physiology and biochemistry of source-regulated protein accumulation in the wheat grain[J]. Journal of Plant Physiology, 2007, 164:581-590. DOI: 10.1016/j.jplph.2006.03.009. doi: 10.1016/j.jplph.2006.03.009
[3]	尤佳琪, 吴明德, 李国庆. 木霉菌在植物病害生物防治中的应用及作用机制[J]. 中国生物防治学报, 2019, 35(6):966-976. DOI: 10.16409/j.cnki.2095-039x.2019.06.025. doi: 10.16409/j.cnki.2095-039x.2019.06.025
	YOU J Q, WU M D, LI G Q. Application and mechanism of Trichoderma in biological control of plant disease[J]. Chinese Journal of Biological Control, 2019, 35(6):966-976. DOI: 10.16409/j.cnki.2095-039x.2019.06.025. doi: 10.16409/j.cnki.2095-039x.2019.06.025
[4]	秦文韬, 陈凯, 庄文颖. 木霉属5个中国新记录种及2种木霉在中国的新分布[J]. 菌物学报, 2016, 35(8):994-1007. DOI: 10.13346/j.mycosystema.150168 doi: 10.13346/j.mycosystema.150168
	QIN W T, CHEN K, ZHUANG W Y. Five Trichoderma species new to China and notes on two other widespread species[J]. Mycosystema, 2016, 35(8):994-1007. DOI: 10.13346/j.mycosystema.150168. doi: 10.13346/j.mycosystema.150168
[5]	IRISY, SRIVASTVA AK, KAPULNIK Y, et al. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants[J]. Plant and Soil, 2001, 235(2):235-242. DOI: 10.1023/A:1011990013955. doi: 10.1023/A:1011990013955
[6]	VALERIE G, ANTOUN H, TWEDDELL R J. Growth stimulation and fruityield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: possible role of indole acetic acid (IAA)[J]. Soil Biology and Biochemistry, 2007, 39(8):1968-1977. DOI: 10.1016/j.soilbio.2007.02.015. doi: 10.1016/j.soilbio.2007.02.015
[7]	SWAIN H, ADAK T, MUKHERJEE A K, et al. Novel, Trichoderma stains.Isolated from tree barks as potential biocontrol agents and biofertilizers fordirect seeded rice[J]. Microbiological Research, 2018, 214:83-90. DOI: 10.1016/j.micres.2018.05.015. doi: 10.1016/j.micres.2018.05.015
[8]	DENGJ J, HUANG W Q, LI Z W, et al. Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi[J]. Enzyme and Microbial Techonology, 2018, 112:35-42. DOI: 10.1016/j.enzmictec.2018.02.002. doi: 10.1016/j.enzmictec.2018.02.002
[9]	吴晓青, 赵晓燕, 赵忠娟, 等. 木霉拮抗灰霉菌对环境pH的影响[J]. 福建农业学报, 2015, 30(9):884-890. DOI: 10.19303/j.issn.1008-0384.2015.09.011. doi: 10.19303/j.issn.1008-0384.2015.09.011
	WU X Q, ZHAO X Y, ZHAO Z J, et al. Correlation between environmental pH and antagonism of Trichoderma harzianum against Botrytis cinerea[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9):884-890. DOI: 10.19303/j.issn.1008-0384.2015.09.011. doi: 10.19303/j.issn.1008-0384.2015.09.011
[10]	ZHANG X, HARVEY P R, STUMMER B E, et al. Antibiosis functions during interactions of Trichoderma afroharzianum and Trichoderma gamsii with plant pathogenic Rhizoctonia and Pythium[J]. Functional & Integrative Genomics. 2015, 15(5):599-610. DOI: 10.1007/s10142-015-0456-x. doi: 10.1007/s10142-015-0456-x
[11]	吴晓青, 吕玉平, 任何, 等. 木霉对草酸耐受和消除作用的初步分析[J]. 微生物学通报, 2016, 43(9):1988-1998. DOI: 10.13344/j.microbiol.china.150692. doi: 10.13344/j.microbiol.china.150692
	WU X Q, LV Y P, REN H, et al. Tolerance and elimination of oxolic acid by Trichoderma spp.[J]. Microbiology China, 2016, 43(9):1988-1998. DOI: 10.13344/j.microbiol.china.150692. doi: 10.13344/j.microbiol.china.150692
[12]	扈进冬, 吴远征, 魏艳丽, 等. 木霉拌种剂对小麦根际土壤真菌群落多样性的影响[J]. 山东科学, 2019, 32(2):46-51. DOI: 10.3976/j.issn.1002-4026.2019.01.007. doi: 10.3976/j.issn.1002-4026.2019.01.007
	HU J D, WU Y Z, WEI Y L, et al. Effects of Trichoderma seed dressing agent on the diversity of fungal community in wheat rhizosphere soil[J]. Shandong Science, 2019, 32(2):46-51. DOI: 10.3976/j.issn.1002-4026.2019.01.007. doi: 10.3976/j.issn.1002-4026.2019.01.007
[13]	吴晓青, 赵忠娟, 李哲, 等. 施加木霉可湿性粉剂对冬小麦田间生长的影响[J]. 山东科学, 2015, 28(6):35-42. DOI: 10.3976/j.issn.1002-4026.2015.06.006. doi: 10.3976/j.issn.1002-4026.2015.06.006
	WU X Q, ZHAO Z J, LI Z, et al. Impact of Trichoderma wettable powder application on winter wheat field growth[J]. Shandong Science, 2015, 28(6):35-42. DOI: 10.3976/j.issn.1002-4026.2015.06.006. doi: 10.3976/j.issn.1002-4026.2015.06.006
[14]	中华人民共和国国家质量技术监督局. 饲料中氨基酸的测定: GB/T 18246—2000[S]. 北京: 中国国家标准出版社, 2000.
	State Bureau of Quality and Technical Supervisionof the People's Republic of China. Determination of amino acids in feeds: GB/T 18246-2000[S]. Beijing: Standards Press of China, 2000.
[15]	中华人民共和国国家市场监督管理总局. 饲料中色氨酸的测定: GB/T 15400—2018[S]. 北京: 中国国家标准出版社, 2018.
	State Administration for Market Regulation of the People's Republic of China. Determination of tryptophan in feeds: GB 15400—2018[S]. Beijing: Standards Press of China, 2018.
[16]	中国人民共和国国家食品药品监督管理总局. 食品安全国家标准食品中蛋白质的测定: GB 5009.5—2016[S]. 北京: 中国国家标准出版社, 2016.
	China Food and Drug Administration of the People's Republic of China. Food Safety National Standard for food: Determination of protein in foods: Kjeldahl method: GB 5009.5—2016[S]. Beijing: Standards Press of China, 2016.
[17]	中国人民共和国国家质量监督检验检疫总局. 小麦和小麦粉面筋含量第1部分: 手洗法测定湿面筋: GB/T 5506.1—2008[S]. 北京: 中国国家标准出版社, 2008.
	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. wheat and wheat flour gluten content part 1: Determination of wet gluten by hand washing-method: GB/T 5506.1—2008[S]. Beijing: Standards Press of China, 2008.
[18]	国家质量技术监督局. 优质小麦强筋小麦: GB/T 17892—1999[S]. 北京: 中国标准出版社, 1999.
	The State Bureau of Quality and Technical Supervision. High quality wheat—Strong gluten wheat: GB/T 17892—1999[S]. Beijing: Standards Press of China, 1999.
[19]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 小麦品种品质分类: GB/T 17320—2013[S]. 北京: 中国标准出版社, 2013.
	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Quality classification of wheat varieties: GB/T 17320—2013[S]. Beijing: Standards Press of China, 2013.
[20]	国家质量技术监督局. 优质小麦弱筋小麦: GB/T 17893—1999[S]. 北京: 中国标准出版社, 1999.
	The State Bureau of Quality and Technical Supervision. High quality wheat—Weak gluten wheat: GB/T 17893—1999[S]. Beijing: Standards Press of China, 1999.
[21]	张林生. 小麦种子氨基酸的评价[J]. 麦类作物学报, 1996, 3:28-30. DOI: 10.7606/j.issn.1009-1041.1996.03.056. doi: 10.7606/j.issn.1009-1041.1996.03.056
	ZHANG L S. Evaluation of amino acids in wheat seeds[J]. Journal of Triticeae Crops, 1996, 3:28-30. DOI: 10.7606/j.issn.1009-1041.1996.03.056. doi: 10.7606/j.issn.1009-1041.1996.03.056
[22]	PEPO P, GYRORI Z. Amino acid compositions in wheat species with different genomes. Cereal Research Communications, 2007, 35:1685-1699. DOI: 10.1556/CRC.35.2007.4.15. doi: 10.1556/CRC.35.2007.4.15
[23]	刘慧, 王朝辉, 李富翠, 等. 不同麦区小麦籽粒蛋白质与氨基酸含量及评价[J]. 作物学报, 2016, 42(5):768-777. DOI: 10.3724/SP.J.1006.2016.00768. doi: 10.3724/SP.J.1006.2016.00768
	LIU H, WANG Z H, LI F C, et al. Contents of protein and amino acids of wheat grain in different wheat production regions and their evaluation[J]. Acta Agronomica Sinica, 2016, 42(5):768-777. DOI: 10.3724/SP.J.1006.2016.00768. doi: 10.3724/SP.J.1006.2016.00768
[24]	刘东军, 宋维富, 杨雪峰, 等. 面筋仪指标在强筋小麦育种及食品加工中的应用[J]. 黑龙江农业科学, 2020(10):120-123. DOI: 10.11942/j.issn1002-2767.2020.10.0120. doi: 10.11942/j.issn1002-2767.2020.10.0120
	LIU D J, SONG W F, YANG X F, et al. Application of Gluten Parameters in Strong Gluten Wheat Breeding and Food Processing[J]. Heilongjiang Agricultural Sciences, 2020(10):120-123. DOI: 10.11942/j.issn1002-2767.2020.10.0120. doi: 10.11942/j.issn1002-2767.2020.10.0120
[25]	李桂萍, 张根生, 巴青松, 等. 杂种小麦品质形状的性状相关和主成分分析[J]. 浙江农业学报, 2016, 28(9):1447-1453. DOI: 10.3969/j.issn.1004-1524.2016.09.01. doi: 10.3969/j.issn.1004-1524.2016.09.01
	LI G P, ZHANG G S, BA Q S, et al. Correlation analysis and principal component analysis on quality traits in hybrid wheat[J]. Acta Agriculturae Zhejiangensis, 2016, 28(9):1447-1453. DOI: 10.3969/j.issn.1004-1524.2016.09.01. doi: 10.3969/j.issn.1004-1524.2016.09.01
[26]	崔文礼, 王军, 汪辉, 等. 黄淮麦区35份小麦品质种质资源品质形状比较分析[J]. 安徽农业大学学报, 2020, 47(4):606-611. DOI: 10.13610/j.cnki.1672-352x.20200907.013. doi: 10.13610/j.cnki.1672-352x.20200907.013
	CUI W L, WANG J, WANG H, et al. Comparative analysis of quality characters of 35 wheat germplasm resources in Huang-huai wheat region[J]. Journal of Anhui Agricultural University, 2020, 47(4):606-611. DOI: 10.13610/j.cnki.1672-352x.20200907.013. doi: 10.13610/j.cnki.1672-352x.20200907.013

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

处理	千粒重/g
CK	42.73±0.08
L-6	43.87±0.12
L-7	44.11±0.12
L-8	43.23±0.19

氨基酸		氨基酸质量分数(%)
氨基酸		CK	L-6	L-7	L-8
必需氨基酸	异亮氨酸	0.27±0.020	0.27±0.020	0.33±0.012	0.32±0.039
	亮氨酸	0.59±0.031	0.62±0.032	0.75±0.005	0.74±0.025
	苯丙氨酸	0.37±0.083	0.47±0.020	0.56±0.048	0.57±0.023
	赖氨酸	0.19±0.022	0.24±0.028	0.29±0.009	0.28±0.007
	缬氨酸	0.34±0.038	0.34±0.026	0.42±0.015	0.39±0.014
	蛋氨酸	0.01±0.001	0.04±0.010	0.03±0.009	0.07±0.010
	色氨酸	0.17±0.020	0.28±0.022	0.26±0.040	0.24±0.017
	苏氨酸	0.24±0.047	0.24±0.035	0.29±0.030	0.28±0.035
必需氨基酸总量		2.18	2.52	2.93	2.89
非必需氨基酸	脯氨酸	0.72±0.028	0.91±0.010	1.09±0.010	1.07±0.160
	酪氨酸	0.22±0.025	0.40±0.007	0.41±0.003	0.36±0.029
	天门冬氨酸	0.39±0.015	0.42±0.010	0.52±0.023	0.5±0.011
	谷氨酸	2.42±0.029	2.70±0.050	3.28±0.170	3.23±0.022
	丝氨酸	0.38±0.008	0.45±0.022	0.55±0.058	0.54±0.009
	甘氨酸	0.32±0.005	0.35±0.004	0.43±0.019	0.42±0.032
	胱氨酸	0.03±0.000	0.02±0.001	0.01±0.001	0.02±0.000
	组氨酸	0.19±0.013	0.23±0.020	0.27±0.021	0.26±0.003
	精氨酸	0.25±0.007	0.27±0.020	0.35±0.050	0.31±0.040
	丙氨酸	0.28±0.006	0.30±0.020	0.36±0.044	0.35±0.008
非必需氨基酸总量		5.20	6.05	7.27	7.06
氨基酸总量		7.38	8.57	10.20	9.95

Effect of Trichoderma harzianum LTR-2 on winter wheat grain quality

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 26

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XIE Yao, ZHANG Zhifei, WANG Quanbo, MU Yan. A study on the role and mechanism of soluble dietary fiber in a mouse constipation model and constipated human population [J]. Shandong Science, 2025, 38(4): 28-38.
[2]	SONG Liuyang, SONG Wenlu, SUI Zhanbin, ZHANG Qi, WANG Jinguo. Ultrasound-induced mutation breeding of high lipid content Chlorella sp. [J]. Shandong Science, 2025, 38(4): 78-85.
[3]	SONG Zeyu, LI Zhenyuan, PAN Tao, MENG Xiangting, LI Song, DONG Hailun, FAN Huaying. Protective effect and mechanism of quercetin on adriamycin-induced nephrotic syndrome in rats [J]. Shandong Science, 2023, 36(4): 61-68.
[4]	SHI Jian-ru,GAO Yao,ZHAO Hui-liang,TIAN Jun-sheng,WU Yan-fei. Nature-effect relationship research of cold bitter and warm pungent medicinal properties of Xiaoyaosan for soothing the liver and relieving depression based on nature combination [J]. Shandong Science, 2022, 35(1): 37-44.
[5]	ZHANG Hua-li, ZHOU Hong-liang, SUN Jia-chang. Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer [J]. Shandong Science, 2021, 34(3): 26-32.
[6]	CAO Ming-chen, YU Zhen-ying, XIN Zhao-yang, XU Long, YANG Zhi-wei, REN Wei, XING Xiao-min, JING Fan-bo. Multicomponent-multitarget-multichannel mechanism of Wulingsan based on network pharmacology [J]. Shandong Science, 2020, 33(1): 51-60.
[7]	CHEN Yang, WANG Yan-wei, YANG Guang-can* . Study on interaction between p53 protein and PBR322 DNA based on atomic force microscopy [J]. SHANDONG SCIENCE, 2017, 30(4): 106-111.
[8]	LI Gao-yan,SUN Zhao-qian,GUO Qing-mei,ZHOU Feng-qin. Nutritional ingredients analysis of four kinds of jujubes [J]. SHANDONG SCIENCE, 2017, 30(3): 33-38.
[9]	HOU Cai-ping, HAN Li-wen, HOU Hai-rong, WANG Xi-min, ZHANG Shan-shan,ZHANG Xuan-ming, WANG Xue, LI Xiao-bin, TIAN Qing-ping, HE Qiu-xia, LIU Ke-chun. The research progress of sarcomere assembly [J]. SHANDONG SCIENCE, 2017, 30(2): 25-36.
[10]	LIANG Xiao-hui,ZHANG Xiao-dong,SI Hong-yu,ZHAO Yu-xiao, HUA Dong-liang,WANG Bing-lian,YAN Yuan-yuan,YANG Li-jun,LU Yong. Resource utilization of protein alkali extraction from municipal residual sewage sludge [J]. SHANDONG SCIENCE, 2016, 29(4): 55-59.
[11]	ZHANG Ying-jie, GEＮＧＹan-ling, WANG Xiao, LIU Feng. Extraction and deproteinization technology for polysaccharide from Dendrobium officinale protocorm [J]. SHANDONG SCIENCE, 2016, 29(3): 7-12.
[12]	WU Xiaoqing,ZHAO Zhongjuan,LI Zhe,HU Jindong,ZHAO Xiaoyan,WANG Yilian,HUANG Yujie,LI Jishun,YANG Hetong. Impact of Trichoderma wettable powder application on winter wheat field growth [J]. SHANDONG SCIENCE, 2015, 28(6): 35-42.
[13]	LIN Yun, ZHANG Shaolong,ZHANG Qinggang, ZHANG Yici. Rapid simulation of protein microsecond level folding process [J]. SHANDONG SCIENCE, 2015, 28(3): 96-103.
[14]	SHI Yan, LIU Qian, ZHANG Yong-qing, LI Jia. Amino acid comparative study of Caprae seu ovis cornu from five different production areas [J]. SHANDONG SCIENCE, 2014, 27(5): 14-17.
[15]	SUN Shi-Qing, WANG Shao-Jie, LI Qiu-Shun, LI Xue-Mei, SHI Jian-Guo. Coomassie brilliant blue method based protein content determination in milk [J]. Shandong Science, 2011, 24(6): 53-55.