Shandong Science ›› 2021, Vol. 34 ›› Issue (6): 62-67.doi: 10.3976/j.issn.1002-4026.2021.06.008
• Microbiology of Agriculture • Previous Articles Next Articles
LI Ling(),LIU Bao-jun,YANG Kai,CHEN Kai,WANG Yi-lian,LI Ji-shun(
)
Received:
2021-01-13
Online:
2021-12-20
Published:
2021-12-13
Contact:
LI Ji-shun
E-mail:yewu2@sdas.org
CLC Number:
LI Ling, LIU Bao-jun, YANG Kai, CHEN Kai, WANG Yi-lian, LI Ji-shun. Effect of Trichoderma harzianum LTR-2 on winter wheat grain quality[J].Shandong Science, 2021, 34(6): 62-67.
Table 2
Effect of spraying of T. harzianum LTR-2 suspension on grain amino acid content of winter wheat"
氨基酸 | 氨基酸质量分数(%) | ||||
---|---|---|---|---|---|
CK | L-6 | L-7 | L-8 | ||
必需氨基酸 | 异亮氨酸 | 0.27±0.020 | 0.27±0.020 | 0.33±0.012 | 0.32±0.039 |
亮氨酸 | 0.59±0.031 | 0.62±0.032 | 0.75±0.005 | 0.74±0.025 | |
苯丙氨酸 | 0.37±0.083 | 0.47±0.020 | 0.56±0.048 | 0.57±0.023 | |
赖氨酸 | 0.19±0.022 | 0.24±0.028 | 0.29±0.009 | 0.28±0.007 | |
缬氨酸 | 0.34±0.038 | 0.34±0.026 | 0.42±0.015 | 0.39±0.014 | |
蛋氨酸 | 0.01±0.001 | 0.04±0.010 | 0.03±0.009 | 0.07±0.010 | |
色氨酸 | 0.17±0.020 | 0.28±0.022 | 0.26±0.040 | 0.24±0.017 | |
苏氨酸 | 0.24±0.047 | 0.24±0.035 | 0.29±0.030 | 0.28±0.035 | |
必需氨基酸总量 | 2.18 | 2.52 | 2.93 | 2.89 | |
非必需氨基酸 | 脯氨酸 | 0.72±0.028 | 0.91±0.010 | 1.09±0.010 | 1.07±0.160 |
酪氨酸 | 0.22±0.025 | 0.40±0.007 | 0.41±0.003 | 0.36±0.029 | |
天门冬氨酸 | 0.39±0.015 | 0.42±0.010 | 0.52±0.023 | 0.5±0.011 | |
谷氨酸 | 2.42±0.029 | 2.70±0.050 | 3.28±0.170 | 3.23±0.022 | |
丝氨酸 | 0.38±0.008 | 0.45±0.022 | 0.55±0.058 | 0.54±0.009 | |
甘氨酸 | 0.32±0.005 | 0.35±0.004 | 0.43±0.019 | 0.42±0.032 | |
胱氨酸 | 0.03±0.000 | 0.02±0.001 | 0.01±0.001 | 0.02±0.000 | |
组氨酸 | 0.19±0.013 | 0.23±0.020 | 0.27±0.021 | 0.26±0.003 | |
精氨酸 | 0.25±0.007 | 0.27±0.020 | 0.35±0.050 | 0.31±0.040 | |
丙氨酸 | 0.28±0.006 | 0.30±0.020 | 0.36±0.044 | 0.35±0.008 | |
非必需氨基酸总量 | 5.20 | 6.05 | 7.27 | 7.06 | |
氨基酸总量 | 7.38 | 8.57 | 10.20 | 9.95 |
[1] |
ANJUM F M, AHMAD I, BUTT M S, et al. Amino acid composition of spring wheats and losses of lysine during chapati baking[J]. Journal of Food Composition and Analysis, 2005, 18:523-532. DOI: 10.1016/j.jfca.2004.04.009.
doi: 10.1016/j.jfca.2004.04.009 |
[2] |
BARNEIXA J. Physiology and biochemistry of source-regulated protein accumulation in the wheat grain[J]. Journal of Plant Physiology, 2007, 164:581-590. DOI: 10.1016/j.jplph.2006.03.009.
doi: 10.1016/j.jplph.2006.03.009 |
[3] |
尤佳琪, 吴明德, 李国庆. 木霉菌在植物病害生物防治中的应用及作用机制[J]. 中国生物防治学报, 2019, 35(6):966-976. DOI: 10.16409/j.cnki.2095-039x.2019.06.025.
doi: 10.16409/j.cnki.2095-039x.2019.06.025 |
YOU J Q, WU M D, LI G Q. Application and mechanism of Trichoderma in biological control of plant disease[J]. Chinese Journal of Biological Control, 2019, 35(6):966-976. DOI: 10.16409/j.cnki.2095-039x.2019.06.025.
doi: 10.16409/j.cnki.2095-039x.2019.06.025 |
|
[4] |
秦文韬, 陈凯, 庄文颖. 木霉属5个中国新记录种及2种木霉在中国的新分布[J]. 菌物学报, 2016, 35(8):994-1007. DOI: 10.13346/j.mycosystema.150168
doi: 10.13346/j.mycosystema.150168 |
QIN W T, CHEN K, ZHUANG W Y. Five Trichoderma species new to China and notes on two other widespread species[J]. Mycosystema, 2016, 35(8):994-1007. DOI: 10.13346/j.mycosystema.150168.
doi: 10.13346/j.mycosystema.150168 |
|
[5] |
IRISY, SRIVASTVA AK, KAPULNIK Y, et al. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants[J]. Plant and Soil, 2001, 235(2):235-242. DOI: 10.1023/A:1011990013955.
doi: 10.1023/A:1011990013955 |
[6] |
VALERIE G, ANTOUN H, TWEDDELL R J. Growth stimulation and fruityield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: possible role of indole acetic acid (IAA)[J]. Soil Biology and Biochemistry, 2007, 39(8):1968-1977. DOI: 10.1016/j.soilbio.2007.02.015.
doi: 10.1016/j.soilbio.2007.02.015 |
[7] |
SWAIN H, ADAK T, MUKHERJEE A K, et al. Novel, Trichoderma stains.Isolated from tree barks as potential biocontrol agents and biofertilizers fordirect seeded rice[J]. Microbiological Research, 2018, 214:83-90. DOI: 10.1016/j.micres.2018.05.015.
doi: 10.1016/j.micres.2018.05.015 |
[8] |
DENGJ J, HUANG W Q, LI Z W, et al. Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi[J]. Enzyme and Microbial Techonology, 2018, 112:35-42. DOI: 10.1016/j.enzmictec.2018.02.002.
doi: 10.1016/j.enzmictec.2018.02.002 |
[9] |
吴晓青, 赵晓燕, 赵忠娟, 等. 木霉拮抗灰霉菌对环境pH的影响[J]. 福建农业学报, 2015, 30(9):884-890. DOI: 10.19303/j.issn.1008-0384.2015.09.011.
doi: 10.19303/j.issn.1008-0384.2015.09.011 |
WU X Q, ZHAO X Y, ZHAO Z J, et al. Correlation between environmental pH and antagonism of Trichoderma harzianum against Botrytis cinerea[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9):884-890. DOI: 10.19303/j.issn.1008-0384.2015.09.011.
doi: 10.19303/j.issn.1008-0384.2015.09.011 |
|
[10] |
ZHANG X, HARVEY P R, STUMMER B E, et al. Antibiosis functions during interactions of Trichoderma afroharzianum and Trichoderma gamsii with plant pathogenic Rhizoctonia and Pythium[J]. Functional & Integrative Genomics. 2015, 15(5):599-610. DOI: 10.1007/s10142-015-0456-x.
doi: 10.1007/s10142-015-0456-x |
[11] |
吴晓青, 吕玉平, 任何, 等. 木霉对草酸耐受和消除作用的初步分析[J]. 微生物学通报, 2016, 43(9):1988-1998. DOI: 10.13344/j.microbiol.china.150692.
doi: 10.13344/j.microbiol.china.150692 |
WU X Q, LV Y P, REN H, et al. Tolerance and elimination of oxolic acid by Trichoderma spp.[J]. Microbiology China, 2016, 43(9):1988-1998. DOI: 10.13344/j.microbiol.china.150692.
doi: 10.13344/j.microbiol.china.150692 |
|
[12] |
扈进冬, 吴远征, 魏艳丽, 等. 木霉拌种剂对小麦根际土壤真菌群落多样性的影响[J]. 山东科学, 2019, 32(2):46-51. DOI: 10.3976/j.issn.1002-4026.2019.01.007.
doi: 10.3976/j.issn.1002-4026.2019.01.007 |
HU J D, WU Y Z, WEI Y L, et al. Effects of Trichoderma seed dressing agent on the diversity of fungal community in wheat rhizosphere soil[J]. Shandong Science, 2019, 32(2):46-51. DOI: 10.3976/j.issn.1002-4026.2019.01.007.
doi: 10.3976/j.issn.1002-4026.2019.01.007 |
|
[13] |
吴晓青, 赵忠娟, 李哲, 等. 施加木霉可湿性粉剂对冬小麦田间生长的影响[J]. 山东科学, 2015, 28(6):35-42. DOI: 10.3976/j.issn.1002-4026.2015.06.006.
doi: 10.3976/j.issn.1002-4026.2015.06.006 |
WU X Q, ZHAO Z J, LI Z, et al. Impact of Trichoderma wettable powder application on winter wheat field growth[J]. Shandong Science, 2015, 28(6):35-42. DOI: 10.3976/j.issn.1002-4026.2015.06.006.
doi: 10.3976/j.issn.1002-4026.2015.06.006 |
|
[14] | 中华人民共和国国家质量技术监督局. 饲料中氨基酸的测定: GB/T 18246—2000[S]. 北京: 中国国家标准出版社, 2000. |
State Bureau of Quality and Technical Supervisionof the People's Republic of China. Determination of amino acids in feeds: GB/T 18246-2000[S]. Beijing: Standards Press of China, 2000. | |
[15] | 中华人民共和国国家市场监督管理总局. 饲料中色氨酸的测定: GB/T 15400—2018[S]. 北京: 中国国家标准出版社, 2018. |
State Administration for Market Regulation of the People's Republic of China. Determination of tryptophan in feeds: GB 15400—2018[S]. Beijing: Standards Press of China, 2018. | |
[16] | 中国人民共和国国家食品药品监督管理总局. 食品安全国家标准食品中蛋白质的测定: GB 5009.5—2016[S]. 北京: 中国国家标准出版社, 2016. |
China Food and Drug Administration of the People's Republic of China. Food Safety National Standard for food: Determination of protein in foods: Kjeldahl method: GB 5009.5—2016[S]. Beijing: Standards Press of China, 2016. | |
[17] | 中国人民共和国国家质量监督检验检疫总局. 小麦和小麦粉面筋含量第1部分: 手洗法测定湿面筋: GB/T 5506.1—2008[S]. 北京: 中国国家标准出版社, 2008. |
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. wheat and wheat flour gluten content part 1: Determination of wet gluten by hand washing-method: GB/T 5506.1—2008[S]. Beijing: Standards Press of China, 2008. | |
[18] | 国家质量技术监督局. 优质小麦强筋小麦: GB/T 17892—1999[S]. 北京: 中国标准出版社, 1999. |
The State Bureau of Quality and Technical Supervision. High quality wheat—Strong gluten wheat: GB/T 17892—1999[S]. Beijing: Standards Press of China, 1999. | |
[19] | 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 小麦品种品质分类: GB/T 17320—2013[S]. 北京: 中国标准出版社, 2013. |
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Quality classification of wheat varieties: GB/T 17320—2013[S]. Beijing: Standards Press of China, 2013. | |
[20] | 国家质量技术监督局. 优质小麦弱筋小麦: GB/T 17893—1999[S]. 北京: 中国标准出版社, 1999. |
The State Bureau of Quality and Technical Supervision. High quality wheat—Weak gluten wheat: GB/T 17893—1999[S]. Beijing: Standards Press of China, 1999. | |
[21] |
张林生. 小麦种子氨基酸的评价[J]. 麦类作物学报, 1996, 3:28-30. DOI: 10.7606/j.issn.1009-1041.1996.03.056.
doi: 10.7606/j.issn.1009-1041.1996.03.056 |
ZHANG L S. Evaluation of amino acids in wheat seeds[J]. Journal of Triticeae Crops, 1996, 3:28-30. DOI: 10.7606/j.issn.1009-1041.1996.03.056.
doi: 10.7606/j.issn.1009-1041.1996.03.056 |
|
[22] |
PEPO P, GYRORI Z. Amino acid compositions in wheat species with different genomes. Cereal Research Communications, 2007, 35:1685-1699. DOI: 10.1556/CRC.35.2007.4.15.
doi: 10.1556/CRC.35.2007.4.15 |
[23] |
刘慧, 王朝辉, 李富翠, 等. 不同麦区小麦籽粒蛋白质与氨基酸含量及评价[J]. 作物学报, 2016, 42(5):768-777. DOI: 10.3724/SP.J.1006.2016.00768.
doi: 10.3724/SP.J.1006.2016.00768 |
LIU H, WANG Z H, LI F C, et al. Contents of protein and amino acids of wheat grain in different wheat production regions and their evaluation[J]. Acta Agronomica Sinica, 2016, 42(5):768-777. DOI: 10.3724/SP.J.1006.2016.00768.
doi: 10.3724/SP.J.1006.2016.00768 |
|
[24] |
刘东军, 宋维富, 杨雪峰, 等. 面筋仪指标在强筋小麦育种及食品加工中的应用[J]. 黑龙江农业科学, 2020(10):120-123. DOI: 10.11942/j.issn1002-2767.2020.10.0120.
doi: 10.11942/j.issn1002-2767.2020.10.0120 |
LIU D J, SONG W F, YANG X F, et al. Application of Gluten Parameters in Strong Gluten Wheat Breeding and Food Processing[J]. Heilongjiang Agricultural Sciences, 2020(10):120-123. DOI: 10.11942/j.issn1002-2767.2020.10.0120.
doi: 10.11942/j.issn1002-2767.2020.10.0120 |
|
[25] |
李桂萍, 张根生, 巴青松, 等. 杂种小麦品质形状的性状相关和主成分分析[J]. 浙江农业学报, 2016, 28(9):1447-1453. DOI: 10.3969/j.issn.1004-1524.2016.09.01.
doi: 10.3969/j.issn.1004-1524.2016.09.01 |
LI G P, ZHANG G S, BA Q S, et al. Correlation analysis and principal component analysis on quality traits in hybrid wheat[J]. Acta Agriculturae Zhejiangensis, 2016, 28(9):1447-1453. DOI: 10.3969/j.issn.1004-1524.2016.09.01.
doi: 10.3969/j.issn.1004-1524.2016.09.01 |
|
[26] |
崔文礼, 王军, 汪辉, 等. 黄淮麦区35份小麦品质种质资源品质形状比较分析[J]. 安徽农业大学学报, 2020, 47(4):606-611. DOI: 10.13610/j.cnki.1672-352x.20200907.013.
doi: 10.13610/j.cnki.1672-352x.20200907.013 |
CUI W L, WANG J, WANG H, et al. Comparative analysis of quality characters of 35 wheat germplasm resources in Huang-huai wheat region[J]. Journal of Anhui Agricultural University, 2020, 47(4):606-611. DOI: 10.13610/j.cnki.1672-352x.20200907.013.
doi: 10.13610/j.cnki.1672-352x.20200907.013 |
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