基于原子力显微镜研究p53蛋白与PBR322 DNA的相互作用

doi:10.3976/j.issn.1002-4026.2017.04.017

Abstract

Abstract:

Tumor (cancer) is caused by the apoptosis or proliferation of cells when DNA damage cannot be repaired in time in the process of replication. The processes of DNA transcription and expression are all involved in the interaction of protein and DNA in living cells. And, tumor suppressor protein is also one of the key proteins involved in this process. However, many studies have found that the tumor suppressor protein p53 can recognize and repair damagingDNA, which plays a significant role in cell apoptosis, gene protection and avoidance of cancer. Studies have shown that the structural stability of p53 protein and the affinity of p53DNA can be enhanced by metal ions, such as magnesium and zinc ions. Therefore, based on atomic force microscopy (AFM), the interaction image of p53 protein and PBR322 ring DNA is visually presented. And, it is found that p53 protein can promote a ring DNA aggregation or cross in itself. However, this phenomenon occurrence is rare for linear DNA with the length of 5 000bp, and it is not occurrence in 20 000bp DNA either. However, protein p53 can make ring DNA to be a twist shape in high magnesium concentration solution, that is, the supercoil shape is formed. This phenomenon may provide guidance for the study on the function and mechanism of protein p53, as well as provide insights into cancer therapy, cancer drug development and cancer detection methods.

Key words: PBR322 ring DNA, DNA supercoil, protein p53, interaction, metal magnesium ions

CLC Number:

CHEN Yang, WANG Yan-wei, YANG Guang-can* . Study on interaction between p53 protein and PBR322 DNA based on atomic force microscopy[J].SHANDONG SCIENCE, 2017, 30(4): 106-111.

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Study on interaction between p53 protein and PBR322 DNA based on atomic force microscopy

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