SHANDONG SCIENCE ›› 2016, Vol. 29 ›› Issue (4): 55-59.doi: 10.3976/j.issn.1002-4026.2016.04.012

• Biomass Energy • Previous Articles     Next Articles

Resource utilization of protein alkali extraction from municipal residual sewage sludge

LIANG Xiao-hui 1,ZHANG Xiao-dong1*,SI Hong-yu1,ZHAO Yu-xiao1, HUA Dong-liang1,WANG Bing-lian2,YAN Yuan-yuan3,YANG Li-jun4,LU Yong1   

  1. 1. Shandong Provincial Key Laboratory of Biomass Gasification Technology, Energy Research Institute, Shandong Academy of
     Sciences,Jinan 250014, China; 2. Biology Institute, Shandong Academy of Sciences,Jinan 250014, China; 3. Jiangsu Green
    Square Garden Biological Technology Co., Ltd.,Yancheng 224000, China; 4.Shandong Guanbaolin
    Carbon Industry Group Co.,Ltd., Qingdao 266300, China
  • Received:2016-06-16 Online:2016-08-20 Published:2016-08-20

Abstract:

We address protein extraction process of municipal residual sewage sludge.We initially compared acid and alkaline hydrolysis pretreatment methods and optimized the better alkaline hydrolysis method for protein extraction from municipal residual sludge. To acquire optimal extraction condition, we optimized NaOH concentration, solution amount and pretreatment time with response surface method.The optimal protein extraction condition is 21.79 g wet municipal sludge,80 mL NaOH solution (3.68 mol/L), and pretreatment time of 20.32 h at 50 ℃.The optimal protein extraction yield is 8 967±0.4 μg/g dry sludge, 2.33 times of initial extraction yield.

Key words: optimization, municipal sewage sludge waste, protein extraction, alkaline hydrolysis

CLC Number: 

  • X703.1